The Gracilariaceae Germplasm Bank of the University of São Paulo, Brazil—a DNA barcoding approach

Costa, Emmanuelle S.; Plastino, Estela M.; Petti, R.; Oliveirã, Eurico C.; Oliveira, Mariana Cabral de

doi:10.1007/s10811-012-9828-y

Cited by 35 publications

(19 citation statements)

References 53 publications

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“…Uma das ferramentas moleculares que auxilia a taxonomia é a técnica de "DNA barcoding", que consiste em obter um fragmento relativamente curto de DNA (~400-700 pb), que pode ser inteiramente sequenciado como o mesmo par de "primers" utilizado na PCR, e ser utilizado em uma comparação com um banco de dados para identificar a espécie. Este sistema começou a ser utilizado principalmente em animais (Hebert et al, 2003), mas esse estudo tem aumentado cada vez mais em algas (Clarkston & Saunders, 2010;Costa et al, 2012;Le Gall & Saunders, 2010;Milstein & Saunders, 2012;Milstein et al, 2011;Saunders & McDonald, 2010;Saunders, 2005;Sherwood & Presting, 2007;Sherwood et al, 2008).…”

Section: As Ferramentas Molecularesunclassified

Diversidade de Gelidiales (Rhodophyta) baseada em marcadores moleculares e estudos morfoanatômicos para região Sudeste do Brasil

Iha¹

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Section: As Ferramentas Molecularesunclassified

Diversidade de Gelidiales (Rhodophyta) baseada em marcadores moleculares e estudos morfoanatômicos para região Sudeste do Brasil

Iha¹

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“…Four strains of Gracilaria birdiae (in vitro unialgal and nonaxenic cultures) obtained from the Gracilariaceae Germplasm Bank of the University of São Paulo (Costa et al, 2012) were selected for the experiments as follows. Apical branches of female gametophytes of the red (wild-type, RD CE ) and greenish-brown (GB CE ) strains were derived from algae collected in March 1994 from Paracuru Beach (03.40°S 39.07°W), Ceará State (CE), Brazil (Costa & Plastino, 2011); the red wild-type (RD ES ) strain was derived from algae collected in August 1994 from Anchieta Beach (20.80°S 40.65°W), Espírito Santo State (ES), Brazil (Plastino et al, 2004); and the green (GR CE ) strain was established from tetraspores released by green apical segments that had spontaneously arisen on a red tetrasporophyte obtained in the laboratory in June 1997 (Costa & Plastino, 2011).…”

Section: Biological Materialsmentioning

confidence: 99%

Effects of UV-B radiation on growth rates, pigment content and ultrastructure of red (wild type), greenish-brown and green strains ofGracilaria birdiae(Gracilariales, Rhodophyta)

Ayres-Ostrock

Plastino

2014

European Journal of Phycology

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Based on physiological characteristics, we hypothesized that different strains of Gracilaria birdiae from two distinct geographical areas of the Brazilian coast (2500 km apart) would have different responses to long-term exposure to UV-B radiation (UV-B). The locations differ in their environmental conditions: one is a warmer area, Ceará State (CE), closer to the equator; the other is a colder area, Espirito Santo State (ES), closer to the Tropic of Capricorn. To test the hypothesis that the CE population is more resistant to UV-B than the ES population, apical segments of the red (RD CE , RD ES ), green (GR CE ) and greenish-brown (GB CE ) strains were cultivated in the laboratory under two treatments: control (PAR) and artificial UV-B (PAR + UV-B). Algal performance was evaluated by considering growth rates, pigment content and ultrastructural analysis. Compared with the control, all strains showed a decrease in growth rates after exposure to UV-B. Of all strains, RD ES showed the greatest sensitivity to UV-B. However, a decrease in growth rate and morphological changes were observed to a lesser extent in the RD CE strain. Moreover, exposure to UV-B resulted in a decrease in the concentrations of phycobiliproteins in the RD CE strain. The GB CE strain showed an increase in phycoerythrin (PE)/allophycocyanin (APC) and phycocyanin (PC)/allophycocyanin (APC) ratios after exposure to UV-B, suggesting this strain had a higher tolerance to the radiation. No differences in the chlorophyll a and carotenoid content were found between the control and UV-B treated samples for all strains. Ultrastructural changes, such as damage to chloroplasts and mitochondria, were present in all strains after exposure to UV-B. In summary, our findings support the hypothesis that the population from Ceará State has adapted to the higher irradiation and is thus more resistant to increased UV-B. Additionally, of the strains tested, the GB CE and RD CE strains appear to be more resistant to this radiation.

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“…Nas últimas décadas, essas técnicas têm sido empregadas na identificação de diferentes Gracilariaceae (Bellorin et al, 2002(Bellorin et al, , 2004(Bellorin et al, , 2008Guillermin et al, 2008;Gurgel et al, 2004Gurgel et al, , 2008Destombe et al, 2010;Costa et al, 2012).…”

Section: Marcadores Molecularesunclassified

Estudos populacionais em Gracilaria birdiae e G.caudata (Gracilariales, Rhodophyta): aspectos fenológicos, fisiológicos e moleculares

Avres-Ostrock¹

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The Gracilariaceae Germplasm Bank of the University of São Paulo, Brazil—a DNA barcoding approach

Cited by 35 publications

References 53 publications

Diversidade de Gelidiales (Rhodophyta) baseada em marcadores moleculares e estudos morfoanatômicos para região Sudeste do Brasil

Diversidade de Gelidiales (Rhodophyta) baseada em marcadores moleculares e estudos morfoanatômicos para região Sudeste do Brasil

Effects of UV-B radiation on growth rates, pigment content and ultrastructure of red (wild type), greenish-brown and green strains ofGracilaria birdiae(Gracilariales, Rhodophyta)

Estudos populacionais em Gracilaria birdiae e G.caudata (Gracilariales, Rhodophyta): aspectos fenológicos, fisiológicos e moleculares

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