The group-specific antigen and other structural proteins of hamster and mouse C-type viruses

Oroszlan, Stephen; Foreman, C.; Kelloff, Gary J.; Gilden, Raymond V.

doi:10.1016/0042-6822(71)90290-x

Cited by 93 publications

(70 citation statements)

References 22 publications

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“…Electrophoresis (from right to left) was for 5 hr at 8 ma per gel; gels were stained with Coomassie blue. The purified gs protein (lower pattern) has a calculated molecular weight of 31,000 (13). The region of the second low-molecular weight zone detected by use of radioactive viral proteins show two bands by staining in this run; thus, the reference to the third fastest migrating polypeptide as the major gs protein (see text) is not an absolute definition.…”

Section: Properties Of Gs Antigensmentioning

confidence: 99%

“…2) and corresponded in mobility to the third fastest migrating band of the three low molecular weight polypeptides usually resolved from mammalian C-type viruses (13)(14)(15)(16)(17)(18). Antigenic analysis has revealed that this protein, referred to as the major gs protein, contained speciesspecific determinants (gs-1) (19) and interspecies crossreactive determinants (gs-3) (20) on the same structure (14,15,21).…”

Section: Properties Of Gs Antigensmentioning

confidence: 99%

“…In the case of the four mammalian C-type viruses, it has been possible to isolate a major protein component from each virus (10,(13)(14)(15) and to prepare specific antibody against it mainly in guinea pigs. The key isolation procedure has been the isoelectric focusing technique, which is illustrated for MuLV in Fig.…”

Section: Properties Of Gs Antigensmentioning

confidence: 99%

“…The isoelectric points for the proteins from each of the four mammalian viruses and what we consider to be the homologous protein in avian viruses are given in Table 1. When the purified proteins were analyzed in SDS-polyacrylamide gels, they proved homogeneous (10,13 Disrupted virus and purified gs protein were analyzed in SDS-polyacrylamide gels (10% acrylamide-0.1% SDS-0.1 M sodium phosphate buffer, pH 7.0). Before electrophoresis, the virus and gs protein were treated with SDS (1%), urea (6 M), and 2-mercaptoethanol at 370 for 2 hr.…”

Section: Properties Of Gs Antigensmentioning

confidence: 99%

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Group-Specific Antigens of RNA Tumor Viruses as Markers for Subinfectious Expression of the RNA Virus Genome

Gilden

Oroszlan

1972

Proc. Natl. Acad. Sci. U.S.A.

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Antigenic markers associated with the major internal protein of RNA tumor viruses of the C-type have proven extremely useful in natural history studies of these viruses. This protein possesses species-specific antigenic determinants, and, in the case of mammalian Ctype viruses, the protein possesses crossreactive determinants as well. These determinants are, thus, useful for species identification and classification of mammalian viruses. A unique distribution of antigens in embryonic tissues of several species (where tests are available) was detected, and in addition, antigen expression in tissues appears to be controlled by a dominant gene. These data have contributed greatly to the theory that RNA tumorvirus information is inherited as part of the cellular genome.

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Section: Properties Of Gs Antigensmentioning

confidence: 99%

Section: Properties Of Gs Antigensmentioning

confidence: 99%

Section: Properties Of Gs Antigensmentioning

confidence: 99%

Section: Properties Of Gs Antigensmentioning

confidence: 99%

See 2 more Smart Citations

Group-Specific Antigens of RNA Tumor Viruses as Markers for Subinfectious Expression of the RNA Virus Genome

Gilden

Oroszlan

1972

Proc. Natl. Acad. Sci. U.S.A.

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“…Several of the murine leukemia virus (MuLV) proteins have recently been separated, isolated, and identified as distinct virus antigens (1)(2)(3)(4)(5)(6)(7). The availability of relatively specific, as well as complex, antisera has revealed in complement fixation and immunodiffusion tests several separate group-specific (gs) and one type-specific (v) antigen (6,8).…”

mentioning

confidence: 99%

Activating and Protective Capacities of a Purified Electrophoretic Fraction of Murine Leukemia Virus for Murine Leukemia Virus Infectivity

Fischinger

Lange

Schäfer

1972

Proc. Natl. Acad. Sci. U.S.A.

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A highly purified gel electrophoretic fraction of murine leukemia virus (MuLV) can confer enhanced infectivity on MuLV derived from tissue culture and can protect MuLV from neutralization by specific antiserum. Of the known viral proteins tested, a purified electrophoretic fraction (14,000 daltons) is the only active fraction and seems to consist of the group-specific antigen I and an associated lipid moiety. The action of this fraction obtained from one type of MuLV is group-specific in that it can enhance and protect serologically different types of MuLV. The effect of this fraction is exerted on viruses and not on cells. MuLV derived from tumors was not enhanced by the fraction, but became amenable to its action after a single passage through tissue culture. Reconstruction experiments suggested that the fraction may consist of an exterior protein associated with some lipids. This complex, which apparently protrudes on the viral surface, is required for infectivity and shields the typespecific antigen containing the hemagglutinating site.Several of the murine leukemia virus (MuLV) proteins have recently been separated, isolated, and identified as distinct virus antigens (1-7). The availability of relatively specific, as well as complex, antisera has revealed in complement fixation and immunodiffusion tests several separate group-specific (gs) and one type-specific (v) antigen (6, 8). The new nomenclature based on purified antigen reactivity recognizes the following main antigens: IV, the major gs-antigen of the species-specific type previously described as gs-1; V, the gs-antigen also known as gs-3 shared by leukemia viruses of several mammalian species; III a lesser antigen; II, an antigen complex that contains both a gs-and type-specific determinants and hemagglutinating activity; I, a species-specific gs-antigen that can be lost after treatment with neuraminidase and phospholipase C; and finally component X, which could be identified as a virus-specific structure by complement fixation (6,(8)(9)(10) (80/20), by inoculation of a small volume (0.2 ml) of virus directly into a large volume (5 ml) of supernatant. Lytic-type lesions indicative of MuLV replication were scored as focus-inducing units (FIU) as described (12, 13).Antisera and Neutralization. A GLV-specific G-serum of high titer, "Old," obtained from F1 (WFu X BN) rats carrying syngeneic lymphomas induced by GLV, and an FLY or Rauscher leukemia virus (RLV)-specific antiserum produced in monkeys against plasma RLV, RM-serum, "Fink," were gifts from Drs. L. Old and M. Fink, respectively (1,14

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Immunofluorescent detection of murine and hamster C‐type virus species‐specific (gs‐1) determinants by monospecific guinea‐pig sera and interspecies‐specific (gs‐3) determinants by tumor‐bearing rat sera

Hampar

Gilden

Kelloff

et al. 1971

Intl Journal of Cancer

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The species‐specific (gs‐1) determinants of murine and hamster C‐type viruses were detected by direct immunofluorescence (FA) in fixed virus‐infected cells using monospecific guinea‐pig antisera. The interspecies‐specific (gs‐3) determinants were also detected by FA using broad spectrum sera from tumor‐bearing rats. The FA staining of gs‐1 and gs‐3 determinants in fixed infected cells was localized in the cytoplasm as punctate foci at or near the cell membrane and as a diffuse mass at the perinuclear region. The FA staining of live infected cells was limited to antisera with envelope activity. The percentage of FA positive cells in any one cell preparation was inversely related to the efficiency of complete virus synthesis. Blocking experiments support the contention that the gs‐1 and gs‐3 determinants reside on the same molecule with the former occupying a more accessible or dominant position.

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The group-specific antigen and other structural proteins of hamster and mouse C-type viruses

Cited by 93 publications

References 22 publications

Group-Specific Antigens of RNA Tumor Viruses as Markers for Subinfectious Expression of the RNA Virus Genome

Group-Specific Antigens of RNA Tumor Viruses as Markers for Subinfectious Expression of the RNA Virus Genome

Activating and Protective Capacities of a Purified Electrophoretic Fraction of Murine Leukemia Virus for Murine Leukemia Virus Infectivity

Immunofluorescent detection of murine and hamster C‐type virus species‐specific (gs‐1) determinants by monospecific guinea‐pig sera and interspecies‐specific (gs‐3) determinants by tumor‐bearing rat sera

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