No abstract

The major internal protein of the RD-114 virus that appeared in a human tumor cell line, RD, after passage through fetal cats was purified by isoelectric focusing and used to prepare antibody in guinea pigs.

MATERIALS AND METHODS Murine leukemia virus (MuLV). Several strains of mouse leukemia virus were used in these studies. Rauscher virus (R-MuLV) was grown in monolayer cultures of chronically infected mouse (BALB/c) bone marrow, JLS-V9 cells (50). The virus-shedding cell line was obtained from Electro-Nucleonics, Bethesda, Md. AKR virus was obtained from NIH Swiss embryo cells infected with an isolate from the spleen of an AKR mouse. These cells were kindly provided by Janet Hartley (National Cancer Institute). The La Puente isolate (1504E) of the wild mouse leukemia virus (WMLV) was obtained from Earle Officer (University of Southern California, Los Angeles, Calif.) (31; M.