The growth of human fibroblasts and A431 epidermoid carcinoma cells on gamma-irradiated human amnion collagen substrata

Abstract

Human fibroblasts and A431 human epidermoid carcinoma cells were cultured on gamma-irradiated human amnion collagen as well as on plastic dishes and non-irradiated collagen coated dishes. The morphology, attachment, growth and short-term cytotoxicity of these culture conditions have been determined. Both irradiated and non-irradiated amnion collagen enhanced the attachment and proliferation of fibroblasts as compared to the plastic dishes. No differences in these properties were observed for A431 cells cultured on irradiated collagen when compared with culture on non-irradiated collagen substrates. Cytotoxicity assays showed that irradiated and non-irradiated collagens were not cytotoxic for either fibroblasts or A431 cells. The results demonstrated that amnion collagen irradiated at doses of 0.25-2.0 Mrads is optimal for cell growth.

Comparative Physico-chemical and in Vitro Properties of Fibrillated Collagen Scaffolds from Different Sources

“…We designed reconstituted collagen in the form of scaffolds to minimize the complication during storage and application. We have also utilized FSC, AMC and CSC to assess their bio-application capabilities considering previously published literature [25][26][27][28][29][30][31][32]. From the investigation it is evident that there exists a close relationship between the characteristic behavior of collagen scaffolds and the source from which it was obtained.…”