Irrigation is required to control salinity and drought stress but it is difficult to secure water resources with good quality in reclaimed lands. This study was conducted to 1) examine applicability of river water as irrigation resources, and 2) estimate suitable irrigation amount and nitrogen fertilization rate (NFR) according to root zone condition for kenaf in the Saemangeum reclaimed land. Under the limited rooting depth, kenaf was cultivated with four irrigation levels (600, 800, 1,000 and 1,200 mm) and four NFRs (0, 36, 84 and 120 N kg ha -1 ) in 2020. With the extended rooting depth, kenaf was subjected to same irrigation treatments to those in 2020 and three NFRs (0, 60 and 120 N kg ha -1 ). The Total organic carbon (TOC) and Biochemical oxygen demand (BOD) of river water slightly exceeded the agricultural water quality standard in some periods on 2020 and 2021, but water quality generally met the standard. Kenaf growth and yield increased with irrigation of 1,000 mm, but decreased in 1,200 mm compared to those in 1,000 mm under the limited rooting condition. With extended root zone, kenaf yield continuously increased with irrigation. At irrigation of 1,200 mm, kenaf yield increased with increasing NFR. Consequently, the nitrogen fertilizer effect was amplified as soil moisture was sufficiently supplied through irrigation. It is concluded that the amount of water and fertilizer should be determined according to the environmental conditions in the Saemangeum reclaimed land.