The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice

Jiang, Hao; Wang, Dekai; Wu, Yingbao; Huang, Ke; Duan, Penggen; Li, Na; Xu, Ran; Zeng, Dali; Dong, Guojun; Zhang, Baolan; Zhang, Limin; Inzé, Dirk; Qian, Qian; Li, Yunhai

doi:10.1016/j.molp.2021.04.011

Cited by 104 publications

(50 citation statements)

References 77 publications

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“…Plant architecture, flowering time, leaf angle, and inflorescence architectures all impact yield, grain shape, and size ( Harder and Prusinkiewicz, 2013 ; Sakuma and Schnurbusch, 2020 ). Interestingly, seeds from OsbZIP47KD plants were altered for the length/width (L/W) ratio as compared to WT ( Figures 3I,J ), suggesting OsbZIP47 impedes cell proliferation in the grain width direction, which is supported by a recent finding of Hao et al (2021) . In later sections of our study on dsRNAiOsbZIP47 lines, we identify some molecular links underlying cell proliferation in the developing grain.…”

Section: Resultssupporting

confidence: 70%

“…et al, 2017 ; Ma et al, 2019 ). The effect of OsbZIP47KD on rice grain shape is not reported for maize fea kernels suggesting unique effects of OsbZIP47 on grain size and shape in rice as also reported by Hao et al (2021) . Our transcriptomic analysis identified a set of grain shape genes regulated by OsbZIP47.…”

Section: Discussionmentioning

confidence: 70%

“…Like TCP class1, proteins containing cysteines with low pKa values are sensitive to cellular redox status ( Martins et al, 2018 ). Glutaredoxins (GRX) reduce cellular ROS level and interact with different proteins including AtPAN1, ZmFEA4, and OsbZIP47 to modulate their activity by modifying their redox state ( Li et al, 2009 ; Laporte et al, 2012 ; Schippers et al, 2016 ; Hao et al, 2021 ; Yang et al, 2021 ). Arabidopsis pan and maize fea4 mutants exhibited increased meristem size ( Maier et al, 2011 ; Pautler et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

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OsbZIP47 Is an Integrator for Meristem Regulators During Rice Plant Growth and Development

et al. 2022

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Stem cell homeostasis by the WUSCHEL–CLAVATA (WUS-CLV) feedback loop is generally conserved across species; however, its links with other meristem regulators can be species-specific, rice being an example. We characterized the role of rice OsbZIP47 in vegetative and reproductive development. The knockdown (KD) transgenics showed meristem size abnormality and defects in developmental progression. The size of the shoot apical meristem (SAM) in 25-day OsbZIP47KD plants was increased as compared to the wild-type (WT). Inflorescence of KD plants showed reduced rachis length, number of primary branches, and spikelets. Florets had defects in the second and third whorl organs and increased organ number. OsbZIP47KD SAM and panicles had abnormal expression for CLAVATA peptide-like signaling genes, such as FON2-LIKE CLE PROTEIN1 (FCP1), FLORAL ORGAN NUMBER 2 (FON2), and hormone pathway genes, such as cytokinin (CK) ISOPENTEYLTRANSFERASE1 (OsIPT1), ISOPENTEYLTRANSFERASE 8 (OsIPT8), auxin biosynthesis OsYUCCA6, OsYUCCA7 and gibberellic acid (GA) biosynthesis genes, such as GRAIN NUMBER PER PANICLE1 (GNP1/OsGA20OX1) and SHORTENED BASAL INTERNODE (SBI/OsGA2ox4). The effects on ABBERANT PANICLE ORGANIZATION1 (APO1), OsMADS16, and DROOPING LEAF (DL) relate to the second and third whorl floret phenotypes in OsbZIP47KD. Protein interaction assays showed OsbZIP47 partnerships with RICE HOMEOBOX1 (OSH1), RICE FLORICULA/LEAFY (RFL), and OsMADS1 transcription factors. The meta-analysis of KD panicle transcriptomes in OsbZIP47KD, OsMADS1KD, and RFLKD transgenics, combined with global OSH1 binding sites divulge potential targets coregulated by OsbZIP47, OsMADS1, OSH1, and RFL. Further, we demonstrate that OsbZIP47 redox status affects its DNA binding affinity to a cis element in FCP1, a target locus. Taken together, we provide insights on OsbZIP47 roles in SAM development, inflorescence branching, and floret development.

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Section: Resultssupporting

confidence: 70%

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

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OsbZIP47 Is an Integrator for Meristem Regulators During Rice Plant Growth and Development

et al. 2022

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“…For example, the G protein γ subunit GS3 functions antagonistically with other Gγ subunits, DEP1 and GGC2, and affects grain cell proliferation in rice (Huang et al., 2009; Sun et al., 2018; Zhou et al., 2009). The E3 ubiquitin ligase GW2 is a RING‐type protein that affects grain cell proliferation in rice (Hao et al., 2021; Song et al., 2007). Phytohormones are also involved in controlling grain size.…”

Section: Introductionmentioning

confidence: 99%

KNOX II transcription factor HOS59 functions in regulating rice grain size

Sheng

Wang

et al. 2022

The Plant Journal

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Plant Knotted1-like homeobox (KNOX) genes encode homeodomain-containing transcription factors. In rice (Oryza sativa L.), little is known about the downstream target genes of KNOX Class II subfamily proteins.Here we generated chromatin immunoprecipitation (ChIP)-sequencing datasets for HOS59, a member of the rice KNOX Class II subfamily, and characterized the genome-wide binding sites of HOS59. We conducted trait ontology (TO) analysis of 9705 identified downstream target genes, and found that multiple TO terms are related to plant structure morphology and stress traits. ChIP-quantitative PCR (qPCR) was conducted to validate some key target genes. Meanwhile, our IP-MS datasets showed that HOS59 was closely associated with BELL family proteins, some grain size regulators (OsSPL13, OsSPL16, OsSPL18, SLG, etc.), and some epigenetic modification factors such as OsAGO4a and OsAGO4b, proteins involved in small interfering RNAmediated gene silencing. Furthermore, we employed CRISPR/Cas9 editing and transgenic approaches to generate hos59 mutants and overexpression lines, respectively. Compared with wild-type plants, the hos59 mutants have longer grains and increased glume cell length, a loose plant architecture, and drooping leaves, while the overexpression lines showed smaller grain size, erect leaves, and lower plant height. The qRT-PCR results showed that mutation of the HOS59 gene led to upregulation of some grain size-related genes such as OsSPL13, OsSPL18, and PGL2. In summary, our results indicate that HOS59 may be a repressor of the downstream target genes, negatively regulating glume cell length, rice grain size, plant architecture, etc. The identified downstream target genes and possible interaction proteins of HOS59 improve our understanding of the KNOX regulatory networks.

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“…Numerous studies conducted in rice (Oryza sativa) and Arabidopsis thaliana have provided an insight into the molecular mechanisms controlling seed size (Xing and Zhang, 2010;Valluru et al, 2014;Li et al, 2019). These mechanisms include several signaling pathways that control seed size, such as G-protein signaling, the ubiquitinproteasome pathway, mitogen-activated protein kinase (MAPK) signaling, the HAIKU (IKU) pathway, phytohormones, and some transcriptional regulators (Li and Li, 2016;Li et al, 2019).The ubiquitin proteasome pathway plays an important role in controlling seed size (Li and Li, 2016;Li et al, 2019;Hao et al, 2021). Several RINGtype E3 ubiquitin ligases have been reported to control seed size.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the GW2-Like Genes in Gossypium and Functional Characterization of the Seed Size Effect of GhGW2-2D

Huang

Yang

et al. 2022

Front. Plant Sci.

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Cotton is one of the most economically important crops worldwide. Seed size is a vital trait for plants connected with yield and germination. GW2 encodes a RING_Ubox E3 ubiquitin ligase that controls seed development by affecting cell growth. Here, are few reports on GW2-like genes in cotton, and the function of GW2 in cotton is poorly understood. In the present study, a genome-wide analysis identified 6 and 3 GW2-like genes in each of the two cultivated tetraploids (Gossypium hirsutum and G. barbadense) and each of their diploid ancestral species (G. arboreum, G. raimondii), respectively. GhGW2-2D has the same functional domain and high sequence similarity with AtDA2 in Arabidopsis. Overexpression of GhGW2-2D in Arabidopsis significantly reduced seed and seedling size, suggesting GhGW2-2D is a potential target for regulating cotton seed size. These results provided information on the genetic and molecular basis of GW2-like genes in cotton, thus establishing a foundation for functional studies of cotton seeds.

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The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice

Cited by 104 publications

References 77 publications

OsbZIP47 Is an Integrator for Meristem Regulators During Rice Plant Growth and Development

OsbZIP47 Is an Integrator for Meristem Regulators During Rice Plant Growth and Development

KNOX II transcription factor HOS59 functions in regulating rice grain size

Genome-Wide Analysis of the GW2-Like Genes in Gossypium and Functional Characterization of the Seed Size Effect of GhGW2-2D

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