The HaeIII and XspI PCR-RFLPs detecting polymorphisms at the goat IGFBP-3 locus

Lan, Xianyong; Pan, Chuanying; Chen, H.; Lei, C. Z.; Liu, S.Q.; Zhang, Y.B.; Min, Lu; Li, Jingling; Li, J.Y.; Zhao, Miao; Hu, Shengwu

doi:10.1016/j.smallrumres.2007.01.001

Cited by 16 publications

(19 citation statements)

References 8 publications

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“…Our results showed 8 SNPs in IGFBP-3 gene and were in accordance with the findings reported in Chinese goat (Lan et al 2007). However, we observed 6 nonsynonymous SNPs out of 8 polymorphisms detected but they reported only one nonsynonymous G→C (arg to glu) in the same analyzed fragment.…”

Section: Discussionsupporting

confidence: 92%

“…However, no polymorphism has been detected in exon 2 of IGFBP-3 gene among studied five Indian sheep breeds (Kumar et al 2006), and the results obtained in the study of exon 2 of IGFBP-3 gene were confirmed where no polymorphism was found in respect of this fragment of IGFBP-3 gene in four Egyptian sheep breeds (Hanafy & Salem 2009). Interestingly, one G→C transversion was reported in the same fragment of IGFBP-3 gene analyzed in Chinese goat (Lan et al 2007).…”

Section: Discussionmentioning

confidence: 91%

“…The polymorphisms in the goat IGFBP-3 gene were reported by PCR-SSCP and DNA sequencing method (Lan et al 2007). The present study is the report of polymorphisms in IGFBP-3 gene in Indian goat breeds, wherein 8 SNPs were identified in the exon 2 of IGFBP-3 gene indicating the high level of heterozygosity of this fragment in Indian goat breeds under study.…”

Section: Discussionmentioning

confidence: 99%

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Sequence characterization and genetic variability analysis of GHR, IGF1, and IGFBP-3 genes in nine Indian goat breeds

Sharma¹,

Dutt²,

Jayakumar³

et al. 2014

Journal of Applied Animal Research

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The genes that are part of the somatotropic axis play a crucial role in the regulation of growth and development of goat and other livestock animals. The identification of genetic polymorphisms in these genes will enable us to evaluate the biological relevance of such polymorphisms and to gain a better understanding of quantitative traits like growth. The present study was undertaken with the objective to analyze the caprine growth hormone receptor (GHR), insulin-like growth factor 1 (IGF1), and insulin-like growth factor binding protein 3 (IGFBP-3) genes to gain insight into the polymorphisms in these genes across different indigenous goat breeds in a panel of 80 animals. The comparative gene sequence analysis in all Capra hircus breeds under study revealed 31 single nucleotide polymorphisms (SNPs) across different loci of GHR, IGF1, and IGFBP-3 genes. Out of 31 SNPs detected, 18, 8, and 5 SNPs were present in IGF-1, IGFBP-3, and GHR genes, respectively. All the mutations pointed out in the GHR and IGF1 genes were found to be synonymous and 6 of 8 SNPs in IGFBP-3 were found to be nonsynonymous. All the detected mutations were reported here for the first time. The frequencies of the SNPs were assessed in nine different goat breeds. Across breeds, the average frequency of the least frequent alleles ranged from 0.02 to 0.17.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sequence characterization and genetic variability analysis of GHR, IGF1, and IGFBP-3 genes in nine Indian goat breeds

Sharma¹,

Dutt²,

Jayakumar³

et al. 2014

Journal of Applied Animal Research

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“…For goats, two SNPs (A78G and G217A) in intron 2 have been reported (Lan et al, 2007a(Lan et al, , 2007b.…”

Section: Discussionmentioning

confidence: 99%

“…The full length of the IGFBP3 is 8.9 kb and it contains five encoding exons (Kim et al, 2005). Nucleotide sequences of the IGFBP3 have been determined in cattle (Haegeman et al, 1999;Maciulla et al, 1997;Shukla, 2001;Sun et al, 2002), buffaloes (Kumar et al, 2004;Padma et al, 2004), sheep (Kumar et al, 2002) and goats (Lan et al, 2007a(Lan et al, , 2007b, and association of the IGFBP3 genotypes with production traits has been reported for cattle (Choudhary et al, 2007;Sun et al, 2002). Due to the remoteness of their habitats and to socio-political and ecological factors, the gayal is one of the least studied ungulates (Mohan et al, 2007) and no such information is available for this interesting species.…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms of the insulin-like growth factor-binding protein 3 gene (IGFBP3) in gayal (Bos frontalis)

Fan

et al. 2012

Gene

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Single nucleotide polymorphism in Egyptian cattle insulin-like growth factor binding protein-3 gene

Othman

Alam

El-Aziem

2014

Journal of Genetic Engineering and Biotechnology

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Insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene responsible for the multiple influences of insulin-like growth factors (IGFs) system. It is considered as a candidate gene for growth and production traits. In the present study, we aimed to determine the genetic polymorphism of Egyptian cattle IGFBP-3 gene.The amplified fragment of cattle IGFBP-3 gene at 651-bp was digested with three different endonucleases; HaeIII, MspI and TaqI. The digestion of the PCR products with MspI and TaqI endonucleases revealed similar restriction patterns in all tested animals.Digestion of the PCR product with HaeIII restriction enzyme revealed three different genotypes in Egyptian cattle due to the presence of two alleles; allele A with 7 digested fragments resulting from the presence of 6 restriction sites and allele C with 8 digested fragments resulting from the presence of 7 restriction sites; six sites as allele A in the addition of another restriction site at position 298^299 as a result of SNP (A fi C) in C allele at position 299. The restriction patterns of IGFBP-3/HaeIII showed that forty-six examined animals are genotyped as AA, CC and AC with frequencies of 21.74%, 21.74% and 56.52%, respectively.It is concluded that the IGFBP-3/HaeIII polymorphism may be utilized as a good marker for genetic differentiation between cattle animals for different body functions such as growth, metabolism, reproduction, immunity and energy balance. The nucleotide sequences of Egyptian cattle IGFBP-3 A and C alleles were submitted to GenBank with the accession numbers KF899893 and KF899894, respectively. ª 2014 Production and hosting by Elsevier B.V. on behalf

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The HaeIII and XspI PCR-RFLPs detecting polymorphisms at the goat IGFBP-3 locus

Cited by 16 publications

References 8 publications

Sequence characterization and genetic variability analysis of GHR, IGF1, and IGFBP-3 genes in nine Indian goat breeds

Sequence characterization and genetic variability analysis of GHR, IGF1, and IGFBP-3 genes in nine Indian goat breeds

Polymorphisms of the insulin-like growth factor-binding protein 3 gene (IGFBP3) in gayal (Bos frontalis)

Single nucleotide polymorphism in Egyptian cattle insulin-like growth factor binding protein-3 gene

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