The Hair Follicle: An Underutilized Source of Cells and Materials for Regenerative Medicine

Kiani, Mehrdad T.; Almquist, Benjamin D.

doi:10.1021/acsbiomaterials.7b00072

Cited by 39 publications

(35 citation statements)

References 166 publications

(321 reference statements)

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“…4D). The presence of these appendages can improve the wound healing process, considering that, in the epithelial compartment of the folicles, there are stem cells that can differentiate into numerous cell types 51 . Furthermore, hair folicles are a source of pro-angiogenic factors, such as VEGF 52 .…”

Section: Discussionmentioning

confidence: 99%

Oral administration of EPA-rich oil impairs collagen reorganization due to elevated production of IL-10 during skin wound healing in mice

Bürger

Kühl

Candreva

et al. 2019

Sci Rep

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Wound healing is an essential process for organism survival. Some fatty acids have been described as modulators of wound healing. However, the role of omega-3 fatty acids is unclear. In the present work, we investigate the effects of oral administration of eicosapentaenoic acid (EPA)-rich oil on wound healing in mice. After 4 weeks of EPA-rich oil supplementation (2 g/kg of body weight), mice had increased serum concentrations of EPA (20:5ω-3) (6-fold) and docosahexaenoic acid (DHA; 22:6ω-3) (33%) in relation to control mice. Omega-3 fatty acids were also incorporated into skin in the EPA fed mice. The wound healing process was delayed at the 3 rd and 7 th days after wounding in mice that received EPA-rich oil when compared to control mice but there was no effect on the total time required for wound closure. Collagen reorganization, that impacts the quality of the wound tissue, was impaired after EPA-rich oil supplementation. These effects were associated with an increase of M2 macrophages (twice in relation to control animals) and interleukin-10 (IL-10) concentrations in tissue in the initial stages of wound healing. In the absence of IL-10 (IL-10 −/− mice), wound closure and organization of collagen were normalized even when EPA was fed, supporting that the deleterious effects of EPA-rich oil supplementation were due to the excessive production of IL-10. In conclusion, oral administration of EPA-rich oil impairs the quality of wound healing without affecting the wound closure time likely due to an elevation of the anti-inflammatory cytokine IL-10.

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Section: Discussionmentioning

confidence: 99%

Oral administration of EPA-rich oil impairs collagen reorganization due to elevated production of IL-10 during skin wound healing in mice

Bürger

Kühl

Candreva

et al. 2019

Sci Rep

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“…However, because they readily facilitate bone formation [ 35 ], they may not provide a true representation of ectopic or inappropriate bone formation in an in vitro model. Human dermal papilla (DP) cells are specialised mesenchymal cells found at the base of the hair follicle that play a key role in hair growth and cycling [ [36] , [37] , [38] ], and are accordingly unrelated to bone. Curiously, while not being stem cells, human DP cells do have multipotent tendencies in vitro and can differentiate down both osteogenic and adipogenic lineages when grown in specific differentiation medias [ [39] , [40] , [41] ].…”

Section: Introductionmentioning

confidence: 99%

Demethylation of ITGAV accelerates osteogenic differentiation in a blast-induced heterotopic ossification in vitro cell culture model

Logan

Camman

Williams³

2018

Bone

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Trauma-induced heterotopic ossification is an intriguing phenomenon involving the inappropriate ossification of soft tissues within the body such as the muscle and ligaments. This inappropriate formation of bone is highly prevalent in those affected by blast injuries. Here, we developed a simplified cell culture model to evaluate the molecular events involved in heterotopic ossification onset that arise from the shock wave component of the disease. We exposed three subtypes of human mesenchymal cells in vitro to a single, high-energy shock wave and observed increased transcription in the osteogenic master regulators, Runx2 and Dlx5, and significantly accelerated cell mineralisation. Reduced representation bisulfite sequencing revealed that the shock wave altered methylation of gene promoters, leading to opposing changes in gene expression. Using a drug to target ITGAV, whose expression was perturbed by the shock wave, we found that we could abrogate the deposition of mineral in our model. These findings show how new therapeutics for the treatment of heterotopic ossification can be identified using cell culture models.

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“…19 Beyond the cell types mentioned above, hair follicles contain a number of stem cells at their base that play a role in wound healing. [20][21][22] Si et al found that transfecting hair follicle stem cells with the lncRNA PlncRNA-1 increased their proliferation and differentiation. Treatment of these transfected cells with the TGF-β1 inhibitor LY2109761 decreased these effects, suggesting a role for PlncRNA-1 in the TGF-β1-mediated Wnt/β-catenin signaling cascade.…”

Section: Integumentary Systemmentioning

confidence: 99%

“…Beyond the cell types mentioned above, hair follicles contain a number of stem cells at their base that play a role in wound healing 20‐22 . Si et al found that transfecting hair follicle stem cells with the lncRNA PlncRNA‐1 increased their proliferation and differentiation.…”

Section: Therapeutic Activity Of Lncrna For Tissue Repair and Regenermentioning

confidence: 99%

Therapeutic potential of extracellular vesicle‐associated long noncoding RNA

Born

Harmon

Jay

2020

Bioengineering & Transla Med

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Both extracellular vesicles (EVs) and long noncoding RNAs (lncRNAs) have been increasingly investigated as biomarkers, pathophysiological mediators, and potential therapeutics. While these two entities have often been studied separately, there are increasing reports of EV-associated lncRNA activity in processes such as oncogenesis as well as tissue repair and regeneration. Given the powerful nature and emerging translational impact of other noncoding RNAs such as microRNA (miRNA) and small interfering RNA, lncRNA therapeutics may represent a new frontier. While EVs are natural vehicles that transport and protect lncRNAs physiologically, they can also be engineered for enhanced cargo loading and therapeutic properties. In this review, we will summarize the activity of lncRNAs relevant to both tissue repair and cancer treatment and discuss the role of EVs in enabling the potential of lncRNA therapeutics. K E Y W O R D S exosomes, extracellular vesicles, lncRNA, microparticles, microvesicles lncRNAs play diverse regulatory roles in normal physiological processes. Thus, delivery of lncRNAs via EVs may help in the treatment Abbreviations: CRCs, colorectal carcinoma cells; EVs, extracellular vesicles; hAD-MSCs, human adipose-derived mesenchymal stem cells; HCAECs, human coronary artery endothelial cells; HCC, hepatocellular carcinoma cell; HDFs, human dermal fibroblasts; HDMECs, human dermal microvascular endothelial cells; lncRNA, long noncoding RNA; miRNA, microRNA; SCCs, squamous carcinoma cells.

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The Hair Follicle: An Underutilized Source of Cells and Materials for Regenerative Medicine

Cited by 39 publications

References 166 publications

Oral administration of EPA-rich oil impairs collagen reorganization due to elevated production of IL-10 during skin wound healing in mice

Oral administration of EPA-rich oil impairs collagen reorganization due to elevated production of IL-10 during skin wound healing in mice

Demethylation of ITGAV accelerates osteogenic differentiation in a blast-induced heterotopic ossification in vitro cell culture model

Therapeutic potential of extracellular vesicle‐associated long noncoding RNA

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