The Healing Effect of Bioglue in an Experimental Rabbit Model of Femoral Condyle Articular Cartilage Defect

Abstract

BackgroundThe full-thickness articular cartilage defects of knee have a poor healing capacity that may progress to osteoarthritis and need a knee replacement. This study determines the healing effect of bioglue in fullthickness articular cartilage defect of femoral condyle in rabbit.MethodsForty-eight male rabbits were randomly divided into four equal groups. In group A, 4 mm articular cartilage defects were created in the right and left medial femoral condyles. Then a graft from xiphoid cartilage was transferred into the defect together with a designed bioglue and the knees were closed. In group B, an articular cartilage defect was created identical to group A, but the defect size was 6 mm. In group C, 4 and 6 mm articular cartilage defects were created in the right and left medial femoral condyles respectively. The graft was transferred into the defect and the knees were stitched. In group D, articular cartilage defects were created similar to group C, just filled with bioglue and closed. The rabbits were euthanized and subgroups were defined as A1, B1, C1 and D1 after 30 days and A2, B2, C2 and D2 after 60 days. The cartilages were macroscopically and histologically investigated for any changes.ResultsMicroscopic and macroscopic investigations showed that bioglue had a significant healing effect in the femoral condyle.ConclusionAddition of bioglue can effectively promote the healing of articular cartilage defects.

Comparison of the regenerative effect of adipose‐derived stem cells, fibrin glue scaffold, and autologous bone graft in experimental mandibular defect in rabbit

“…5 Despite this, application of autografts is associated with disadvantages such as providing another surgical site, limited amounts of available bone graft in children and seniors, possibility of bone graft particles moving during their placement, 6 nerve injury, pain, donor site morbidity, and cosmetic concerns. [7][8][9] Although allogeneic and xenogeneic bone grafts have been used as alternatives to the autografts, they have shown some drawbacks such as ethical issues, high cost, immunogenicity, disease transmission, and the possibility of graft rejection, which limit their applications. 10 Research to find an appropriate method to substitute the standard bone graft continues, and extensive studies have focused on bone tissue engineering and regenerative medicine to design and fabricate a suitable scaffold augmented by stem cells and promotive growth factors for healing and reconstruction of bone defects.…”

The Effect of Anadara granosa Shell’s–Stichopus hermanni Scaffold on CD44 and IL-10 Expression to Decrease Osteoclasts in Socket Healing

“…Anesthesia was performed using a Ketamine 10% (Kepro pharmaceuticals, Holland) with a dose of 0.1 mL/kg body weight (BW) and Xyla (Interchemie, the Netherlands) with 0.01 mL/100 g BW on the upper right thigh intramuscularly. 19 Thereafter, the cleaning was performed in the extraction area with water spray and antiseptic fluid to make the extraction area aseptic. Then, the extraction of the lower left mandibular incisive tooth in the rats was performed using needle holders and scaffold applications.…”

Bioadhesives for musculoskeletal tissue regeneration

“…Accordingly, tissue engineering approaches using cells, biomaterial scaffolds, and/or bioactive cues have been widely applied toward cartilage regeneration [103]. Engineering cartilage constructs in vitro followed by in vivo implantation, as well as applying hydrogelbased scaffolds with or without cell and/or growth factors, have been popular regenerative approaches to overcome limitations of the current treatments for cartilage defects [104]. Despite successful engineering of functional cartilaginous tissues in vitro [105], integration with adjacent host tissue has been one of the outstanding challenges to achieve long-lasting success in cartilage regeneration [106].…”