Comparison of the regenerative effect of adipose‐derived stem cells, fibrin glue scaffold, and autologous bone graft in experimental mandibular defect in rabbit

Mehrabani, Davood; Khodakaram-Tafti, Azizollah; Shaterzadeh-Yazdi, Hanieh; Zamiri, Bardia; Omidi, Mahsa

doi:10.1111/edt.12435

Cited by 35 publications

(37 citation statements)

References 33 publications

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“…M esenchymal stem cells (MSCs) are multipotent undifferentiated cells with self-replicating ability that have been isolated from various tissues including bone marrow, 1 adipose, 2 Wharton's jelly, 3 dental pulp, 4 and amniotic fluid (AF). 5 MSCs have self-renewal, differentiation, migratory, anti-inflammatory, and immunosuppressive characteristics, which are needed for cell-based therapies.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Age and Type of Media on Growth Kinetics of Human Amniotic Fluid Stem Cells

Borzou

Mehrabani

Zare

et al. 2020

Biopreservation and Biobanking

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This study compared growth kinetics of human amniotic fluid stem cells (hAFSCs) in different maternal age groups and two different media of AmnioMAX and Dulbecco's modified Eagle's medium (DMEM). Materials and Methods: Three milliliters of amniotic fluid (AF) was provided from 16 pregnant women who were referred for amniocentesis from 16 to 18 weeks of gestation. Mothers were divided to 20-29 (n = 5), 30-39 (n = 5) and 40-49 (n = 6) years old age groups. AF was immediately centrifuged and the cell pellet was cultured. Cells were characterized morphologically, by flow cytometry and for osteogenic and adipogenic inductions. Population doubling time (PDT) and growth kinetics were determined. AFSCs cultured in AmnioMAX were compared in various age groups. A comparison of growth kinetics of AFSCs cultured in AmnioMAX and DMEM from 40 to 49 years old pregnant women was undertaken. Results: AFSCs were adherent to culture flasks and were spindle shape, and positive for osteogenic and adipogenic inductions and for expression of CD73, CD90 and CD105 markers, but negative for CD34 and CD45. PDT among 20-29, 30-39, and 40-49 years old women was 30.9, 38.3, and 43.9 hours, respectively showing a higher cell proliferation in younger ages. When comparing AmnioMAX and DMEM, PDT was 53 and 96.9 hours, respectively denoting to a higher cell proliferation in AmnioMAX. Conclusions: Higher proliferation and plasticity of hAFSCs were noted in AmnioMAX and in younger mothers' samples. These findings can be added to the literature and open a new avenue in regenerative medicine, when hAFSCs are targeted for cell therapy purposes.

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Section: Introductionmentioning

confidence: 99%

The Effect of Age and Type of Media on Growth Kinetics of Human Amniotic Fluid Stem Cells

Borzou

Mehrabani

Zare

et al. 2020

Biopreservation and Biobanking

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“…Autologous grafts containing live bone marrow-derived cells have also been used in the clinic to repair craniofacial defects (Marcacci et al, 2007;Macchiarini et al, 2008;Mehrabani et al, 2018). Human mandibular or maxillary BMSCs demonstrated increased cell proliferation, delayed senescence, and stronger expression of osteoblastic markers as compared to iliac-crest-derived marrow cells from the same patients (Akintoye et al, 2006), suggesting distinct functions and differentiation potential.…”

Section: Introductionmentioning

confidence: 99%

Use of Human Dental Pulp and Endothelial Cell Seeded Tyrosine-Derived Polycarbonate Scaffolds for Robust in vivo Alveolar Jaw Bone Regeneration

Zhang

Saxena

Fakhrzadeh

et al. 2020

Front. Bioeng. Biotechnol.

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The ability to effectively repair craniomaxillofacial (CMF) bone defects in a fully functional and aesthetically pleasing manner is essential to maintain physical and psychological health. Current challenges for CMF repair therapies include the facts that craniofacial bones exhibit highly distinct properties as compared to axial and appendicular bones, including their unique sizes, shapes and contours, and mechanical properties that enable the ability to support teeth and withstand the strong forces of mastication. The study described here examined the ability for tyrosine-derived polycarbonate, E1001(1K)/β-TCP scaffolds seeded with human dental pulp stem cells (hDPSCs) and human umbilical vein endothelial cells (HUVECs) to repair critical sized alveolar bone defects in an in vivo rabbit mandible defect model. Human dental pulp stem cells are uniquely suited for use in CMF repair in that they are derived from the neural crest, which naturally contributes to CMF development. E1001(1k)/β-TCP scaffolds provide tunable mechanical and biodegradation properties, and are highly porous, consisting of interconnected macro-and micropores, to promote cell infiltration and attachment throughout the construct. Human dental pulp stem cells/HUVECs seeded and acellular E1001(1k)/β-TCP constructs were implanted for one and three months, harvested and analyzed by micro-computed tomography, then demineralized, processed and sectioned for histological and immunohistochemical analyses. Our results showed that hDPSC seeded E1001(1k)/β-TCP constructs to support the formation of osteodentinlike mineralized jawbone tissue closely resembling that of natural rabbit jaw bone. Although unseeded scaffolds supported limited alveolar bone regeneration, more robust and homogeneous bone formation was observed in hDPSC/HUVEC-seeded constructs, suggesting that hDPSCs/HUVECs contributed to enhanced bone formation. Importantly, bioengineered jaw bone recapitulated the characteristic morphology of natural rabbit jaw bone, was highly vascularized, and exhibited active remodeling by the presence of osteoblasts and osteoclasts on newly formed bone surfaces. In conclusion, these results demonstrate, for the first time, that E1001(1K)/ β-TCP

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“…However, polysaccharides too like chitosan [112–114] or cornstarch [65, 115] have been investigated for this purpose. In between the animal-derived polymers, ASCs have demonstrated to positively interact mainly with fibrin [116], collagen [117, 118], gelatin [111, 119, 120], and silk [69, 121, 122], through the recognition of specific domains present in polymers' structures. These polymers have a good biodegradability profile, and they can be degraded, modified, or adsorbed by the action of naturally occurring enzymes [123].…”

Section: Scaffolds and Adipose Stem Cellsmentioning

confidence: 99%

Adipose-Derived Stem Cells in Bone Tissue Engineering: Useful Tools with New Applications

Storti

Scioli

Kim

et al. 2019

Stem Cells International

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Adipose stem cells (ASCs) are a crucial element in bone tissue engineering (BTE). They are easy to harvest and isolate, and they are available in significative quantities, thus offering a feasible and valid alternative to other sources of mesenchymal stem cells (MSCs), like bone marrow. Together with an advantageous proliferative and differentiative profile, they also offer a high paracrine activity through the secretion of several bioactive molecules (such as growth factors and miRNAs) via a sustained exosomal release which can exert efficient conditioning on the surrounding microenvironment. BTE relies on three key elements: (1) scaffold, (2) osteoprogenitor cells, and (3) bioactive factors. These elements have been thoroughly investigated over the years. The use of ASCs has offered significative new advancements in the efficacy of each of these elements. Notably, the phenotypic study of ASCs allowed discovering cell subpopulations, which have enhanced osteogenic and vasculogenic capacity. ASCs favored a better vascularization and integration of the scaffolds, while improvements in scaffolds' materials and design tried to exploit the osteogenic features of ASCs, thus reducing the need for external bioactive factors. At the same time, ASCs proved to be an incredible source of bioactive, proosteogenic factors that are released through their abundant exosome secretion. ASC exosomes can exert significant paracrine effects in the surroundings, even in the absence of the primary cells. These paracrine signals recruit progenitor cells from the host tissues and enhance regeneration. In this review, we will focus on the recent discoveries which have involved the use of ASCs in BTE. In particular, we are going to analyze the different ASCs' subpopulations, the interaction between ASCs and scaffolds, and the bioactive factors which are secreted by ASCs or can induce their osteogenic commitment. All these advancements are ultimately intended for a faster translational and clinical application of BTE.

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Comparison of the regenerative effect of adipose‐derived stem cells, fibrin glue scaffold, and autologous bone graft in experimental mandibular defect in rabbit

Abstract: The healing process had a significant increase in the thickness of new cortical bone when fibrin glue scaffold associated with ADSCs was used.

Cited by 35 publications

References 33 publications

The Effect of Age and Type of Media on Growth Kinetics of Human Amniotic Fluid Stem Cells

The Effect of Age and Type of Media on Growth Kinetics of Human Amniotic Fluid Stem Cells

Use of Human Dental Pulp and Endothelial Cell Seeded Tyrosine-Derived Polycarbonate Scaffolds for Robust in vivo Alveolar Jaw Bone Regeneration

Adipose-Derived Stem Cells in Bone Tissue Engineering: Useful Tools with New Applications

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