The Healing Effect of Plantago Major and Aloe Vera Mixture in Excisional Full Thickness Skin Wounds: Stereological Study

Abstract

BACKGROUNDPrevious studies indicated that both Plantago major and Aloe vera have anti-inflammatory, tissue regeneration, antioxidant, and immune-stimulatory effects. It is assumed that a mixture of these two herbal medicines may provide a potent material in treatment of skin wound injuries. Therefore, in this study we investigated the effects of Plantago major and Aloe vera mixture in the process of wound healing in rat models according to stereological parameters. METHODSIn an experiential study, 36 male Sprague-Dawley rats (200±20 g) were randomly assigned into three groups (n=12): The control group which received no treatment, gel base treated group, and the 5% Plantago major and 5% Aloe vera mixture gel treated group (PA group). Treatments were done every 24 hrs for 15 days. Wound closure rate, volume densities of the collagen bundles and the vessels, vessel's length density and mean diameter, and fibroblast populations were estimated using stereological methods. RESULTS PA treated group showed faster wound closure rate in comparison with control and gel-base groups (p<0.05). Numerical density of fibroblasts, volume density of collagen bundles, mean diameter, and volume densities of the vessels in PA group were significantly higher than the control and the gel-base treated groups (p<0.05). CONCLUSIONWe showed that Plantago major and Aloe vera mixture has the ability to improve wound healing by enhancing fibroblast proliferation, collagen bundle synthesis and re-vascularization in skin injuries.

Healing Effect of Plantago major and Photodynamic Therapy Combination on Skin Wounds

“…This finding shows that the administration of laser can lessen the cytotoxicity impact of high concentrations of P. major, indicating an effective synergistic effect of laser and P. major combination. This synergistic effect of combination therapy is in consistent with a recent study, which reported that the mixture of P. major and aloe vera accelerates the process of wound closure by inducing fibroblast proliferation and increasing collagen bundles 22 .…”

Therapeutic Efficacy of Great Plantain (Plantago major L.) in the Treatment of Second-Degree Burn Wounds: A Case-Control Study

“…ey reported that the P. major water extract, especially with 50% concentration, could be a suitable substitute for sulfadiazine silver in burn injury healing and also suggested further studies in humans [20]. Another study demonstrated that the mixture of P. major and Aloe vera gel speeded up the wound recovery time as the result of an increase in the density of vessels, broblasts, and collagen bers in the wound site [21]. Furthermore, in a human study, this gel was found to have signi cantly reduced the ulcer surface of diabetic foot ulcers [22].…”

Effect of aqueous extract of fig fruit (Ficus Carica) on wound healing in albino rabbits

“…Wound healing is one of the most fundamental issues that human beings have faced since the beginning of creation. Numerous drugs and ointments are used to repair open wounds, each of which has several drawbacks, limitations, and side effects (3)(4)(5). Today, the importance of healing is due to the high prevalence of wounds in human and animal communities.…”

“…It is also a complex process that causes restoration of structural and functional integrity after injury (2). Wound healing involves coordinated repair responses of blood cells, extracellular matrix, and parenchymal cells that occur after surgery or traumatic injury to the body (3). Wound healing has long been considered one of the most important issues in surgery.…”