The heart of Daphnia magna: effects of four cardioactive drugs

Villegas-Navarro, A.; Rosas-L, Esperanza; Reyes, José L.

doi:10.1016/s1532-0456(03)00172-8

Cited by 77 publications

(57 citation statements)

References 20 publications

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“…Measurement of cardiac area changes was previously used as an optical method to study the effects of various drugs on heart functioning in Daphnia (Villegas-Navarro et al, 2003). The present study showed that clove oil at the highest concentration increased the heart area during systole when compared to the control values suggesting lower magnitude of heart muscle contraction as a result of heart relaxation.…”

Section: Discussionsupporting

confidence: 45%

“…Daphnia are known to have myogenic heart responding to a variety of agonists and antagonists, similarly to vertebrate animals and optical examination of this organ after treatment with bioactive agents has been used by some authors (Bekker, Krijgsman, 1951;Villegas-Navarro et al, 2003;Campbell et al, 2004;Bownik et al, 2015).This organ is clearly visible through transparent exoskeleton in a light microscope and it is possible to measure its activity. Video analysis of heart functioning in slow motion proved to be a reliable method for a precise determination of heart rate and measure times of cardiac phases without the necessity of slowing down the heart rate by lowering the ambient temperature (Campbell et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Video analysis of heart functioning in slow motion proved to be a reliable method for a precise determination of heart rate and measure times of cardiac phases without the necessity of slowing down the heart rate by lowering the ambient temperature (Campbell et al, 2004). Alteration of heart rate in Daphnia was previously documented as a response to environmental conditions or bioactive substances (Navarro et al, 2003;Campbell et al, 2004;Bownik et al, 2014;Bownik and Stępniewska, 2015) however, there is a lack of data on such effects induced by clove oil.…”

Section: Discussionmentioning

confidence: 99%

“…Longer duration of diastole and increased heart area in systole may be the effect of alterations in functioning of contractile proteins resulting from modification of ion channel activity. (Sensch et al, 2000;Villegas-Navarro et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

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Bownik¹

2015

Turk. J. Fish. Aquat. Sci.

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Clove oil is a common anesthetic used in aquaculture. However little is known on its effects on non-target crustaceans living in fish ponds and other natural reservoirs. The purpose of the investigation was to determine survival, time to anesthesia (TtA) and recovery (TtR), swimming activity, heart functioning during 5 and 45-min exposure of Daphnia magna to clove oil at concentrations of 150, 100, 50, 25, 10 and 1 µl/L. Swimming behavior and heart activity of Daphnia were evaluated by video image analysis with the use of Tracker® v.4.82 and Image Tool® software.The results showed that the oil induced anesthesia at three highest concentrations: 150, 100 and 50 μl/L. TtA and TtR were dependent on the size of the animal and concentration of the oil. The shortest TtA and the longest TtR were noted for the smallest daphnids anaesthetized for 45 minutes at 150, 100 and 50 ul/L. Lethality of small and large daphnids previously exposed to anesthetic concentrations for both times was observed at 24th hour of recovery period suggesting latent toxicity of clove oil. The oil inhibited heart rate, heart muscle contraction and swimming velocity at anesthetic (150, 100, 50 μl/L) and at non-anesthetic concentrations of 25, 10 and 1μl/L. Recovery period after previous 45-min anesthesia showed temporal increase however and subsequent decrease of Daphnia swimming velocity and heart activity resulting probably from latent toxicity.The results of the present study indicate that clove oil induces rapid immobilization and latent toxicity in Daphnia magna, at lower concentrations that those recommended for fish anesthesia and suggest that excessive use of the oil in natural ponds or other water reservoirs may be detrimental to coexisting cladocerans leading to disturbance of ecological balance.

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Section: Discussionsupporting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Bownik¹

2015

Turk. J. Fish. Aquat. Sci.

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“…More attempts have been made to specify other ecological effects which can be used as potential test endpoints. Baylor used Daphnia heart beat rate tests (Baylor, 1942) as early as 1942, which is still considered an applicable endpoint (Villegas-Navarro et al, 2003). Some authors recorded morphological abnormalities (e.g.…”

Section: Introductionmentioning

confidence: 99%

A novel protocol for assessing aquatic pollution, based on the feeding inhibition of Daphnia magna

Kovács

Abdel-Hameid

Ács

et al. 2012

Knowl. Managt. Aquatic Ecosyst.

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In this study, sensitivity of a novel acute bioassay based on the feeding activity of Daphnia magna was assessed, using 2 and 4 h of exposure. For calibration purposes, results were compared with those of the standard immobility test as described by the ISO 6341:1996 standard. Using potassium dichromate as the reference chemical, after 4 h of exposure the proposed protocol showed similar sensitivity in comparison with the standard, as the EC 50 of the immobility test was 1.093 ± 0.098 mg·L -1 , whereas the EC 50 of the feeding inhibition bioassay was 1.742 ± 0.133 mg·L -1 . In order to test the sensitivity of the bioassay, toxicity of two other contaminants, copper and wastewater, was estimated and the results were compared with those of the standard immobility test. For both cases, the feeding inhibition test showed higher sensitivity, as in the case of copper the EC 50s were 0.0952 ± 0.0087 and 0.0753 ± 0.0152 mg·L -1 , whilst the EC 50 recorded for the 24-h immobility test was 0.2407 ± 0.0159 mg·L -1 . In the case of the effluent, EC 50 values after 2 and 4 h of exposure were 15.698 ± 2.681 and 12.557 ± 2.358 expressed as % of the wastewater, respectively, whereas the EC 50 of the immobility test was calculated to be 36.4688 ± 5.4887. RÉSUMÉNouveau protocole pour l'évaluation de la pollution aquatique, basé sur l'inhibition de l'alimentation de Daphnia magna Dans cette étude, la sensibilité d'un nouveau test biologique aiguë basé sur l'activité d'alimentation de Daphnia magna a été évaluée, utilisant 2 et 4 h d'exposition. À des fins d'étalonnage, les résultats ont été comparés à ceux de l'essai standard d'inhibition de la mobilité tel que décrit par la norme ISO 6341:1996 standard. En utilisant le potassium dichromate comme produit chimique de référence, après 4 h d'exposition, le protocole proposé a montré une sensibilité similaire en comparaison de la norme, alors que la EC 50 du test d'inhibition de la mobilité était 1,093 ± 0,098 mg·L -1 , la EC 50 de l'essai biologique d'inhibition d'alimentation a été 1,742 ± 0,133 mg·L -1 . Afin de tester la sensibilité du test biologique, la toxicité de deux autres contaminants, du cuivre et des eaux usées, a été estimée et les résultats ont été comparés à ceux du test de l'inhibition de mobilité standard. Pour les deux cas, le test d'inhibition d'alimentation a montré une sensibilité plus élevée. Dans le cas du cuivre, les EC 50 ont été 0,0952 ± 0,0087 et 0,0753 ± 0,0152 mg·L -1 , alors que la

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Partial least square and k‐nearest neighbor algorithms for improved 3D quantitative spectral data–activity relationship consensus modeling of acute toxicity

Stoyanova-Slavova

Slavov

Pearce

et al. 2014

Enviro Toxic and Chemistry

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A diverse set of 154 chemicals that included US Food and Drug Administration-regulated compounds tested for their aquatic toxicity in Daphnia magna were modeled by a 3-dimensional quantitative spectral data-activity relationship (3D-QSDAR). Two distinct algorithms, partial least squares (PLS) and Tanimoto similarity-based k-nearest neighbors (KNN), were used to process bin occupancy descriptor matrices obtained after tessellation of the 3D-QSDAR space into regularly sized bins. The performance of models utilizing bins ranging in size from 2 ppm × 2 ppm × 0.5 Å to 20 ppm × 20 ppm × 2.5 Å was explored. Rigorous quality-control criteria were imposed: 1) 100 randomized 20% hold-out test sets were generated and the average R(2) test of the respective models was used as a measure of their performance, and 2) a Y-scrambling procedure was used to identify chance correlations. A consensus between the best-performing composite PLS model using 0.5 Å × 14 ppm × 14 ppm bins and 10 latent variables (average R(2) test = 0.770) and the best composite KNN model using 0.5 Å × 8 ppm × 8 ppm and 2 neighbors (average R(2) test = 0.801) offered an improvement of about 7.5% (R(2) test consensus = 0.845). Projection of the most frequently occurring bins on the standard coordinate space indicated that the presence of a primary or secondary amino group-substituted aromatic systems-would result in an increased toxic effect in Daphnia. The presence of a second aromatic ring with highly electronegative substituents 5 Å to 7 Å apart from the first ring would lead to a further increase in toxicity.

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The heart of Daphnia magna: effects of four cardioactive drugs

Cited by 77 publications

References 20 publications

Untitled

Untitled

A novel protocol for assessing aquatic pollution, based on the feeding inhibition of Daphnia magna

Partial least square and k‐nearest neighbor algorithms for improved 3D quantitative spectral data–activity relationship consensus modeling of acute toxicity

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