The heat shock 70 genes of the olive pest <i>Bactrocera oleae</i>: genomic organization and molecular characterization of a transcription unit and its proximal promoter region

Drosopoulou, Elena; Chrysopoulou, Anastasia ChrysopoulouA.; Nikita, Venetia NikitaV.; Mavragani-Tsipidou, Penelope

doi:10.1139/g08-110

Cited by 11 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Clone WHDc0267 (accession number DQ675542) was 192 bp long and its predicted protein sequence had 79% homology with a partial predicted cytochrome P450 in Acyrthosiphon pisum (Harris) (pea aphid) (accession number XP_001944205) and 75% homology with the cytochrome P450 CYP4G25 of Antheraea yamamai (Guerin-Meneville) (silkmoth) (accession number BAD81026). The clone CMDc002_C03 (accession number AB475104) was 179 bp long with a predicted protein sequence that had 61% homology to a partial sequence of a heat shock 70 protein in Bactrocera oleae or Dacus oleae (Gmelin) (olive fruit fly) (accession number CAI44197) (Drosopoulou et al 2009). Clone WHDc0146 (accession number DQ675540) was 550 bp long with 89% and 87% homology to a heat shock cognate 70 protein in Pyrrhocoris apterus (L) (fire bug) (accession number ACJ12782) (Kostal & Tollarova-Borovanska 2009) and a partial sequence of a heat shock cognate 70 in Bemisia tabaci (Gennadius) (whitefly) (accession number AAZ17399), respectively.…”

Section: Resultsmentioning

confidence: 99%

Gene Response to Stress in the Asian Citrus Psyllid (Hemiptera: Psyllidae)

Marutani-Hert¹,

Hunter²,

Hall³

2010

Florida Entomologist

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Gene Response to Stress in the Asian Citrus Psyllid (Hemiptera: Psyllidae)

Marutani-Hert¹,

Hunter²,

Hall³

2010

Florida Entomologist

View full text Add to dashboard Cite

“…A genomic clone (pBohsp70-3 0 ), containing the 3 0 fragment of a B. oleae hsp70 gene (Drosopoulou et al 2009) was labeled using random priming with digoxigenated dUTP (Dig-11dUTP, ROCHE Diagnostics, Mannheim, Germany). Hybridization was performed at 55°C, and the signal was detected with specific antibodies (Anti-Digoxigenin-AP, Fab Fragments, ROCHE Diagnostics, Mannheim, Germany) after the addition of the chromogenic substrates NBT and BCIP (Applichem, Germany), according to Drosopoulou and Scouras (1995).…”

Section: In Situ Hybridization Proceduresmentioning

confidence: 99%

Cytogenetic analysis of the Ethiopian fruit fly Dacus ciliatus (Diptera: Tephritidae)

et al. 2011

View full text Add to dashboard Cite

The Ethiopian fruit fly, Dacus ciliatus, is an important pest of cucurbits, which recently invaded the Middle East. The genetics and cytogenetics of D. ciliatus have been scarcely studied. Such information is, however, an essential basis for understanding the biology of insect pests, as well as for the design of modern control strategies. We report here the mitotic karyotype and detailed photographic maps of the salivary gland polytene chromosomes of this species. The mitotic metaphase complement consists of six pairs of chromosomes, including one pair of heteromorphic sex (XX/XY) chromosomes. The heterogametic sex is ascribed to the male. The analysis of the salivary gland polytene complement shows a total number of five long chromosomes (10 polytene arms), which correspond to the five autosomes of the mitotic nuclei, and a heterochromatic mass corresponding to the sex chromosomes. Banding patterns, as well as the most characteristic features and prominent landmarks of each polytene chromosome are presented and discussed. Chromosomal homologies between D. ciliatus and Bactrocera oleae are proposed by comparing chromosome banding patterns and by in situ hybridization of the hsp70 gene.

show abstract

“…Since the nomenclature of the mitotic and polytene elements was based exclusively on their relative size in descending order, no correlation between the two chromosome sets exists [39] . A large number of ESTs [17] , microsatellites [41] and other molecular markers [42] , [43] have been mapped on B. oleae polytene chromosomes by in situ hybridization. However, none of them proved to be informative on mitotic chromosomes.…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization and Chromosomal Distribution of a Species-Specific Transcribed Centromeric Satellite Repeat from the Olive Fruit Fly, Bactrocera oleae

et al. 2013

Self Cite

View full text Add to dashboard Cite

Satellite repetitive sequences that accumulate in the heterochromatin consist a large fraction of a genome and due to their properties are suggested to be implicated in centromere function. Current knowledge of heterochromatic regions of Bactrocera oleae genome, the major pest of the olive tree, is practically nonexistent. In our effort to explore the repetitive DNA portion of B. oleae genome, a novel satellite sequence designated BoR300 was isolated and cloned. The present study describes the genomic organization, abundance and chromosomal distribution of BoR300 which is organized in tandem, forming arrays of 298 bp-long monomers. Sequence analysis showed an AT content of 60.4%, a CENP-B like-motif and a high curvature value based on predictive models. Comparative analysis among randomly selected monomers demonstrated a high degree of sequence homogeneity (88% – 97%) of BoR300 repeats, which are present at approximately 3,000 copies per haploid genome accounting for about 0.28% of the total genomic DNA, based on two independent qPCR approaches. In addition, expression of the repeat was also confirmed through RT-PCR, by which BoR300 transcripts were detected in both sexes. Fluorescence in situ hybridization (FISH) of BoR300 on mitotic metaphases and polytene chromosomes revealed signals to the centromeres of two out of the six chromosomes which indicated a chromosome-specific centromeric localization. Moreover, BoR300 is not conserved in the closely related Bactrocera species tested and it is also absent in other dipterans, but it’s rather restricted to the B. oleae genome. This feature of species-specificity attributed to BoR300 satellite makes it a good candidate as an identification probe of the insect among its relatives at early development stages.

show abstract

The heat shock 70 genes of the olive pest Bactrocera oleae: genomic organization and molecular characterization of a transcription unit and its proximal promoter region

Cited by 11 publications

References 19 publications

Gene Response to Stress in the Asian Citrus Psyllid (Hemiptera: Psyllidae)

Gene Response to Stress in the Asian Citrus Psyllid (Hemiptera: Psyllidae)

Cytogenetic analysis of the Ethiopian fruit fly Dacus ciliatus (Diptera: Tephritidae)

Molecular Characterization and Chromosomal Distribution of a Species-Specific Transcribed Centromeric Satellite Repeat from the Olive Fruit Fly, Bactrocera oleae

Contact Info

Product

Resources

About