The hector G-Protein Coupled Receptor Is Required in a Subset of fruitless Neurons for Male Courtship Behavior

Li, Yuanli; Hoxha, Valbona; Lama, Chamala; Dinh, Bich Hien; Vo, Christina N.; Dauwalder, Brigitte

doi:10.1371/journal.pone.0028269

Cited by 23 publications

(24 citation statements)

References 49 publications

(67 reference statements)

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“…Notably, we identified MB-specific upregulation of four G-protein coupled receptors (GPCR). These included oamb, hec, and SIFaR, all known to be involved in male courtship behavior ( Li et al 2011 ; Sellami and Veenstra 2015 ; Zhou et al 2012 ), and DopEcR, an atypical GPCR that responds to both dopamine and ecdysone, and is essential for cAMP signal activation during courtship memory ( Ishimoto et al 2013 ). We identified five MB-up genes encoding components of the heterotrimeric G-protein complex (Gαq, Gβ13F, Gγ30A, Gαo, Gγ1), which acts directly downstream GPCRs to induce adenylate cyclase activity and production of cAMP ( Livingstone et al 1984 ; Levin et al 1992 ).…”

Section: Resultsmentioning

confidence: 99%

Mushroom Body Specific Transcriptome Analysis Reveals Dynamic Regulation of Learning and Memory Genes After Acquisition of Long-Term Courtship Memory in Drosophila

Jones¹,

Nixon²,

Chubak³

et al. 2018

G3 Genes|Genomes|Genetics

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The formation and recall of long-term memory (LTM) requires neuron activity-induced gene expression. Transcriptome analysis has been used to identify genes that have altered expression after memory acquisition, however, we still have an incomplete picture of the transcriptional changes that are required for LTM formation. The complex spatial and temporal dynamics of memory formation creates significant challenges in defining memory-relevant gene expression changes. The Drosophila mushroom body (MB) is a signaling hub in the insect brain that integrates sensory information to form memories across several different experimental memory paradigms. Here, we performed transcriptome analysis in the MB at two time points after the acquisition of LTM: 1 hr and 24 hr. The MB transcriptome was compared to biologically paired whole head (WH) transcriptomes. In both, we identified more transcript level changes at 1 hr after memory acquisition (WH = 322, MB = 302) than at 24 hr (WH = 23, MB = 20). WH samples showed downregulation of developmental genes and upregulation of sensory response genes. In contrast, MB samples showed vastly different changes in transcripts involved in biological processes that are specifically related to LTM. MB-downregulated genes were highly enriched for metabolic function. MB-upregulated genes were highly enriched for known learning and memory processes, including calcium-mediated neurotransmitter release and cAMP signaling. The neuron activity inducible genes Hr38 and sr were also specifically induced in the MB. These results highlight the importance of sampling time and cell type in capturing biologically relevant transcript level changes involved in learning and memory. Our data suggests that MB cells transiently upregulate known memory-related pathways after memory acquisition and provides a critical frame of reference for further investigation into the role of MB-specific gene regulation in memory.

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Section: Resultsmentioning

confidence: 99%

Mushroom Body Specific Transcriptome Analysis Reveals Dynamic Regulation of Learning and Memory Genes After Acquisition of Long-Term Courtship Memory in Drosophila

Jones¹,

Nixon²,

Chubak³

et al. 2018

G3 Genes|Genomes|Genetics

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“…To clarify if the sexual dimorphism observed in LN2- Gal4 was determined by the sex of the cell or by the peculiar nature of this enhancer, we feminized these cells by co-expressing a construct from the transformer gene, UAS-tra F [40]. In this genotype, the feminized males still show the high number of LN2-Gal4 expressing neurons as regular males do (electronic supplementary material, figure S6 i , j ).…”

Section: Resultsmentioning

confidence: 99%

Drosophilaenhancer-Gal4 lines show ectopic expression during development

Casas‐Tintó¹,

Arnés²,

Ferrús³

2017

R. Soc. open sci.

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In Drosophila melanogaster the most widely used technique to drive gene expression is the binary UAS/Gal4 system. We show here that a set of nervous system specific enhancers (elav, D42/Toll-6, OK6/RapGAP1) display ectopic activity in epithelial tissues during development, which is seldom considered in experimental studies. This ectopic activity is variable, unstable and influenced by the primary sequence of the enhancer and the insertion site in the chromosome. In addition, the ectopic activity is independent of the protein expressed, Gal4, as it is reproduced also with the expression of Gal80. Another enhancer, LN2 from the sex lethal (Sxl) gene, shows sex-dependent features in its ectopic expression. Feminization of LN2 expressing males does not alter the male specific pattern indicating that the sexual dimorphism of LN2 expression is an intrinsic feature of this enhancer. Other X chromosome enhancers corresponding to genes not related to sex determination do not show sexual dimorphism in their ectopic expressions. Although variable and unstable, the ectopic activation of enhancer-Gal4 lines seems to be regulated in terms of tissue and intensity. To characterize the full domain of expression of enhancer-Gal4 constructs is relevant for the design of transgenic animal models and biotechnology tools, as well as for the correct interpretation of developmental and behavioural studies in which Gal4 lines are used.

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“…Cryosections of whole flies were fixed in 4% Paraformaldehyde and immunohistochemistry performed as described in [ 30 ]. Double staining using anti-ßGal and anti-JHAMT antibodies was performed on the sections.…”

Section: Methodsmentioning

confidence: 99%

Juvenile Hormone Is Required in Adult Males for Drosophila Courtship

2016

Self Cite

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Juvenile Hormone (JH) has a prominent role in the regulation of insect development. Much less is known about its roles in adults, although functions in reproductive maturation have been described. In adult females, JH has been shown to regulate egg maturation and mating. To examine a role for JH in male reproductive behavior we created males with reduced levels of Juvenile Hormone Acid O-Methyl Transferase (JHAMT) and tested them for courtship. JHAMT regulates the last step of JH biosynthesis in the Corpora Allata (CA), the organ of JH synthesis. Males with reduced levels of JHAMT showed a reduction in courtship that could be rescued by application of Methoprene, a JH analog, shortly before the courtship assays were performed. In agreement with this, reducing JHAMT conditionally in mature flies led to courtship defects that were rescuable by Methoprene. The same result was also observed when the CA were conditionally ablated by the expression of a cellular toxin. Our findings demonstrate that JH plays an important physiological role in the regulation of male mating behavior.

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The hector G-Protein Coupled Receptor Is Required in a Subset of fruitless Neurons for Male Courtship Behavior

Cited by 23 publications

References 49 publications

Mushroom Body Specific Transcriptome Analysis Reveals Dynamic Regulation of Learning and Memory Genes After Acquisition of Long-Term Courtship Memory in Drosophila

Mushroom Body Specific Transcriptome Analysis Reveals Dynamic Regulation of Learning and Memory Genes After Acquisition of Long-Term Courtship Memory in Drosophila

Drosophilaenhancer-Gal4 lines show ectopic expression during development

Juvenile Hormone Is Required in Adult Males for Drosophila Courtship

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