The heteropolysaccharide of Mangifera indica fruit: Isolation, chemical profile, complexation with β-lactoglobulin and antioxidant activity

Banerjee, Pallabi; Jana, Subrata; Mukherjee, Shuvam; Bera, Kaushik; Majee, Sujay Kumar; Ali, Imran; Pal, Saikat; Ray, Bimalendu; Ray, Sayani

doi:10.1016/j.ijbiomac.2020.09.161

Cited by 10 publications

(5 citation statements)

References 37 publications

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“…The third stage of mass loss was between 350 and 600°C, and the percentages of weight loss were found to be 25.5% (SHP-1), 24.5% (SHP-2), and 12.8% (SHP-3). The loss of quality at this stage is relatively small and might be related to the oxidation of carbonaceous organic material ( 16 , 47 ). In general, if the polymers in the sample are relatively stable, then only the small molecular weight molecules are lost below 300°C in the nitrogen environment.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

et al. 2022

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Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides. In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%. Three purified polysaccharide fractions were obtained after decolorization, deproteinization, and column chromatography. Then, their main composition and antioxidant activity were investigated. The dominant fraction was SHP-2 with a yield of 3.78%. It was composed of galacturonic acid (51.3%), glucuronic acid (13.8%), rhamnose (8.9%), glucose (8.4%), and others. The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked. This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

et al. 2022

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“…The removal of low molecular weight compounds, exclusion of starch and protein, and then isolation of the polysaccharides by precipitation, or drying are the succeeding steps. To date, sulfated polysaccharides, which generally exert an evidently high potential of bioactivities, have most frequently been extracted from seaweed biomass by conventional water extraction methods [24,[36][37][38], but a long extraction time, poor yield, low selectivity and a lack of automation are the associated drawbacks [39,40]. Heparin is the oldest clinically used anticoagulant isolated from porcine intestine and bovine lung [41].…”

Section: Classical and Modern Extraction Techniquesmentioning

confidence: 99%

“…Precise structures of these purified oligosaccharides may be determined by sugar compositional analysis, glycosidic linkage composition, periodate oxidation, NMR, MALDI-TOF-MS or other methods [125][126][127]. Recently, a method based on ESI-MS analysis of peracetylated oligomeric portions, as derived from polysaccharides by Smith degradation or by enzyme hydrolysis followed by acetylation, has been described as an expedient tool for providing critical structural information on a spectrum of oligosaccharides [37,[128][129][130][131]. Moreover, nuclear magnetic resonance (NMR) spectroscopy is an effective, non-invasive method in general studies on biopolymers, and NMR is a particularly valuable technique in the determination of molecular structures of polysaccharides [132][133][134][135][136][137][138][139][140].…”

Section: Techniques For Structural Characterization Of Polysaccharidesmentioning

confidence: 99%

Exploiting the Amazing Diversity of Natural Source-Derived Polysaccharides: Modern Procedures of Isolation, Engineering, and Optimization of Antiviral Activities

et al. 2020

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Naturally occurring polysaccharide sulfates are highly diverse, owning variations in the backbone structure, linkage pattern and stereochemistry, branching diversity, sulfate content and positions of sulfate group(s). These structural characteristics bring about diverse sulfated polymers with dissimilar negative charge densities and structure–activity relationships. Herein, we start with a short discussion of techniques needed for extraction, purification, chemical sulfation, and structural characterization of polysaccharides. Processes of isolation and sulfation of plant-derived polysaccharides are challenging and usually involve two steps. In this context, we describe an integrated extraction-sulfation procedure that produces polysaccharide sulfates from natural products in one step, thereby generating additional pharmacological activities. Finally, we provide examples of the spectrum of natural source-derived polysaccharides possessing specific features of bioactivity, in particular focusing on current aspects of antiviral drug development and drug–target interaction. Thus, the review presents a detailed view on chemically engineered polysaccharides, especially sulfated derivatives, and underlines their promising biomedical perspectives.

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“…Currently, the high PCP content in P. cyrtonema is highly praised in the Chinese herbal medicine market [ 4 ]. The identification of numerous PCPs with medicinal properties, including antiviral [ 5 , 6 ], antioxidant [ 7 , 8 , 9 ], antitumoral [ 10 ], and immunomodulatory activities [ 11 , 12 , 13 , 14 ], has sparked significant interest in the field of pharmaceutical research and development. PCPs typically exhibit characteristics, such as a large molecular weight (MW) and a repetitive structure, which are often not found in conventional small molecule pharmaceuticals [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Profiling Reveals Two WRKY Transcription Factors Positively Regulating Polysaccharide Biosynthesis in Polygonatum cyrtonema

Jiang,

Chen,

Duan

et al. 2023

IJMS

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Polygonatum cyrtonema (P. cyrtonema) is a valuable rhizome-propagating traditional Chinese medical herb. Polysaccharides (PCPs) are the major bioactive constituents in P. cyrtonema. However, the molecular basis of PCP biosynthesis in P. cyrtonema remains unknown. In this study, we measured the PCP contents of 11 wild P. cyrtonema germplasms. The results showed that PCP content was the highest in Lishui Qingyuan (LSQY, 11.84%) and the lowest in Hangzhou Lin’an (HZLA, 7.18%). We next analyzed the transcriptome profiles of LSQY and HZLA. Through a qRT-PCR analysis of five differential expression genes from the PCP biosynthesis pathway, phosphomannomutase, UDP-glucose 4-epimerase (galE), and GDP-mannose 4,6-dehydratase were determined as the key enzymes. A protein of a key gene, galE1, was localized in the chloroplast. The PCP content in the transiently overexpressed galE1 tobacco leaves was higher than in the wild type. Moreover, luciferase and Y1H assays indicated that PcWRKY31 and PcWRKY34 could activate galE1 by binding to its promoter. Our research uncovers the novel regulatory mechanism of PCP biosynthesis in P. cyrtonema and is critical to molecular-assisted breeding.

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The heteropolysaccharide of Mangifera indica fruit: Isolation, chemical profile, complexation with β-lactoglobulin and antioxidant activity

Cited by 10 publications

References 37 publications

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

Exploiting the Amazing Diversity of Natural Source-Derived Polysaccharides: Modern Procedures of Isolation, Engineering, and Optimization of Antiviral Activities

Comparative Transcriptome Profiling Reveals Two WRKY Transcription Factors Positively Regulating Polysaccharide Biosynthesis in Polygonatum cyrtonema

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