The high dose of inulin exacerbated food allergy through the excess accumulation of short-chain fatty acids in a BABL/c mouse model

Xie, Qiang; Mu, Kaiyu; Chen, Chen; Gu, Shimin; Luo, Dan; Fu, Wenhui; Xue, Wei

doi:10.1016/j.ijbiomac.2023.123234

Cited by 11 publications

(20 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, slightly alkaline pH of colonic contents occurs in the case of colitis and secretory diarrhea . In addition, increased colonic pH has been reported to be associated with allergic responses and intestinal inflammation in OVA-sensitized mice . In this study, we found a unique colonic pH regulation pattern in GS mice.…”

Section: Discussionsupporting

confidence: 51%

“…31 In addition, increased colonic pH has been reported to be associated with allergic responses and intestinal inflammation in OVA-sensitized mice. 7 In this study, we found a unique colonic pH regulation pattern in GS mice. Typically, determinants of colonic pH include carbohydrate fermentation-derived SCFA production, mucosal absorption of SCFAs, luminal bicarbonate and lactate production, and possibly intestinal transit.…”

Section: Discussionsupporting

confidence: 50%

“…Measurements of gluten-specific IgE and IgG1 were performed by indirect ELISA, as described previously. 7 2.5. Analysis of Pro-and Anti-Inflammatory Cytokines in the Spleen.…”

Section: Evaluations Of Clinical Symptomsmentioning

confidence: 99%

“…Measurements of pH and Antioxidant Capacity of the Ileal and Colonic Contents. The intestinal content supernatant was obtained according to the method of Xie et al 7 Briefly, intestinal contents were mixed with 0.01 mM PBS at a ratio of 1:9 (w/v), followed by vertical oscillation. Samples were centrifuged at 12 000g for 15 min to obtain the supernatant.…”

Section: Evaluations Of Clinical Symptomsmentioning

confidence: 99%

“…6 For example, in ovalbumin (OVA)-sensitized mice, luminal pH in both small and large intestines was significantly increased, combined with gut microbiota dysbiosis, impaired intestinal antioxidant capacity, and increased intestinal epithelial permeability. 7 Serial determinations of gluten-induced allergic phenotypes revealed dynamic changes in IMFs, which were underlined by elevated colonic luminal pH and malonaldehyde, decreased superoxide dismutase (SOD) activity, suppressed short-chain fatty acid production, and microbial community alterations. 8 It has also been reported that the intestinal epithelial barrier and gut microbial structure were severely disrupted in response to OVA sensitizations, which was remarkably alleviated by the oral administration of fucoxanthin.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Combination of Gut Microbial Features and the Proteomic Pattern Revealed Changes in Specific Intestinal Luminal Factors and Mechanisms of Their Regulation of Gluten Allergy

Xie

Liu

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Recent research consensus has highlighted the role of intestinal luminal factors in the association between intestinal microenvironment homeostasis and food allergy. However, the association between intestinal immune homeostasis and food allergy-related proteomic features remains elusive. In this study, we aimed to investigate the changes in gluten allergy (GA)-defined phenotypes and endotypes and intestinal microenvironment factors in BALB/c mice and linked GA to colonic proteomic signatures. Combined with increased allergy and diarrhea scores, intense antibody responses and abnormalities in T-cell cytokine production were induced in mice. GA-associated disruption of intestinal microenvironment homeostasis was underlined by the increased colonic pH, decreased intestinal antioxidant capacity, impaired intestinal barrier function, and decreased production and imbalanced proportions of short-chain fatty acids. 16S rRNA amplicon sequencing showed that the gut microbiota dysbiosis in mice was characterized by significant enrichment of six bacterial taxonomic units, including Prevotellaceae, Escherichia Shigella, Alloprevotella, Escherichia coli, Bacteroides vulgatus, and Lachnospiraceae bacterium DW59, which was correlated with immune end points. Using a label-free proteomics quantitative approach, 24 differentially expressed proteins linking GA-induced gut dysbiosis were identified, with four of them enriched in the serine endopeptidase inhibitor activity pathway. The development of GA in mice was associated with changes in specific intestinal luminal factors and may be mediated by serine protease activity-associated metabolic routes.

show abstract

Section: Discussionsupporting

confidence: 51%

Section: Discussionsupporting

confidence: 50%

“…Measurements of gluten-specific IgE and IgG1 were performed by indirect ELISA, as described previously. 7 2.5. Analysis of Pro-and Anti-Inflammatory Cytokines in the Spleen.…”

Section: Evaluations Of Clinical Symptomsmentioning

confidence: 99%

Section: Evaluations Of Clinical Symptomsmentioning

confidence: 99%