The high molecular weight glutenin subunit composition in old and modern bread wheats cultivated in Iran

Abstract

All current and old wheat cultivars grown in Iran were characterised by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). The high-molecular-weight glutenin subunit (HMW-GS) banding patterns for each cultivar were assigned a Glu-1 quality score, a theoretical quality score based on Payne’s Glu-1 quality assignments. At the Glu-A1 loci, HMW-GS subunit compositions N, 7 + 8, 2 + 12 and 2*, 7 + 8, 2 + 12 were found to be predominant being expressed in 24 and 15 cultivars, respectively, out of 95. Eighteen different alleles were identified for the 3 loci studied: Glu-A1 (3), Glu-B1 (9), and Glu-D1 (6). The glutenin quality scores of Iranian wheat ranged from 4 to 10, with an average of 7.4. It was found that some cultivars were heterogeneous in HMW-GS composition. In cv. Cooleh, only one glutenin subunit at the Glu-B1 locus was present. HMW-GS 2*** + 12′ was found in 6 cultivars and biotypes. The results obtained here describing the allelic composition of bread wheat commonly grown in Iran may be useful in wheat breeding programs selecting for good quality parameters.

Allelic variation and genetic diversity of high molecular weight glutenin subunit in Chinese endemic wheats (Triticum aestivum L.)

“…Payne (1987) and Lukow et al (1989) assigned quality scores to individual HMW-GS or HMW-GS pairs, making it possible to predict the breadmaking quality of wheat on the basis of HMW-GS composition. In addition, HMW-GS are convenient genetic markers for estimating genetic variation between wheat lines and species (Margiotta et al 1993;Bushehri et al 2006).…”

10.5937/ratpov50-5098 = High molecular weight (HMW) glutenin subunit composition of NS wheat cultivars released in 1987-2008

“…Thus, the area of northern Europe (Sweden, the Baltic countries) is characterised by the combinations HMW-GS, 2*, 6+8 or 7+9, and 2 + 12 (Johansson et al 1995), and N or 1, 7+9 and 5+10 (Johansson et al 2003), respectively. In Asian countries (China, Iran, India) there is the dominance of HMW-GS N, 7+8, 2+12 (Hua et al 2009); 2*, 7 + 8, 2 + 12 (Shahnejat- Bushehri et al 2006), and 2*, 7, 2+12 (Kalaiselvi & Reddy 2003), respectively. In France the characteristic combination is N, 7+8 or 6+8, 2+12 (Branlard et al 2003), and in Russia N, 7+9, 5+10 (Morgunov et al 1990;Zhenghui et al 2009).…”