The ‘high‐risk’ neuropathological autopsy in AIDS and Creutzfeldt‐Jakob disease: principles and practice

Ironside, James W.; Bell, Jeanne E.

doi:10.1111/j.1365-2990.1996.tb00908.x

Cited by 32 publications

(17 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of the relevant strict Health and Safety procedures in dealing with fresh tissue it was not considered possible to use prion disease cases in the evaluation [12]. All the blocks were cut at 7 μm thickness and stained with haematoxylin and eosin (H&E).…”

Section: Methodsmentioning

confidence: 99%

Simulated surgical-type cerebral biopsies from post-mortem brains allows accurate neuropathological diagnoses in the majority of neurodegenerative disease groups

King

Maekawa

Bódi

et al. 2013

acta neuropathol commun

View full text Add to dashboard Cite

BackgroundIn theory, cerebral biopsies could provide the diagnosis in a significant proportion of patients with neurodegenerative diseases, however, there are considerable ethical barriers. Previous series of cerebral biopsies have shown variable diagnostic accuracy but have understandably suffered because of lack of post-mortem tissue with which to compare the diagnosis. To determine the accuracy of such biopsies in neurodegenerative disease we took small biopsy-sized samples of predominantly fresh post-mortem brain tissue from frontal and temporal lobes in 62 cases. These were processed as for a biopsy and stained for H&E, p62, tau, Aβ, α-synuclein, and TDP-43. The sections were assessed blind by 3 neuropathologists and the results compared with the final post-mortem diagnosis.ResultsThe agreement and sensitivity in most cases was good especially: controls; Alzheimer’s disease (AD); multiple system atrophy (MSA); frontotemporal lobar degeneration with TDP-43 positive inclusions and/or motor neurone disease (FTLD-TDP/MND); Huntington’s disease (HD); corticobasal degeneration (CBD) / microtubular associated protein tau mutation cases with CBD-like features (CBD/MAPT); and combined AD- Dementia with Lewy Bodies (AD-DLB) where the sensitivity on assessing both brain regions varied between 75-100%. There was poor sensitivity for progressive supranuclear palsy (PSP) and amyotrophic lateral sclerosis (ALS) (both 0%), but moderate sensitivity for pure DLB (60%). The temporal lobe assessment was marginally more accurate than the frontal lobe but these were only slightly worse than both combined.ConclusionsThe study shows that with certain caveats the cerebral biopsy in life should be a viable method of accurately diagnosing many neurodegenerative diseases.

show abstract

Section: Methodsmentioning

confidence: 99%

Simulated surgical-type cerebral biopsies from post-mortem brains allows accurate neuropathological diagnoses in the majority of neurodegenerative disease groups

King

Maekawa

Bódi

et al. 2013

acta neuropathol commun

View full text Add to dashboard Cite

show abstract

“…It is resistant to formalin and withstands the conventional autoclaving. Dissection of fresh tissue is performed under a biohazard hood and discarded according to the policy for handling contagious material (Bell and Ironside 1997;Ironside and Bell 1996;Salai et al 1997). The risk of dealing with infectious material (e.g.…”

Section: Safety Measuresmentioning

confidence: 99%

Standard Operating Procedures, ethical and legal regulations in BTB (Brain/Tissue/Bio) banking: what is still missing?

Ravid¹

2007

Cell Tissue Banking

View full text Add to dashboard Cite

The use of human biological specimens in scientific research is the focus of current international public and professional concern and a major issue in bioethics in general. Brain/Tissue/Bio banks (BTB-banks) are a rapid developing sector; each of these banks acts locally as a steering unit for the establishment of the local Standard Operating Procedures (SOPs) and the legal regulations and ethical guidelines to be followed in the procurement and dissemination of research specimens. An appropriat Code of Conduct is crucial to a successful operation of the banks and the research application they handle. What are we still missing ? (1) Adequate funding for research BTB-banks. (2) Standard evaluation protocls for audit of BTB-bank performance. (3) Internationally accepted SOP's which will facilitate exchange and sharing of specimens and data with the scientific community. (4) Internationally accepted Code of Conduct. In the present paper we review the most pressing organizational, methodological, medico-legal and ethical issues involved in BTB-banking; funding, auditing, procurement, management/handling, dissemination and sharing of specimens, confidentiality and data protection, genetic testing, "financial gain" and safety measures. Taking into consideration the huge variety of the specimens stored in different repositories and the enormous differences in medico-legal systems and ethics regulations in different countries it is strongly recommend that the health-care systems and institutions who host BTB-Banks will put more efforts in getting adequate funding for the infrastructure and daily activities. The BTB-banks should define evaluation protocols, SOPs and their Code of Conduct. This in turn will enable the banks to share the collected specimens and data with the largest possible number of researchers and aim at a maximal scientific spin-off and advance in public health research.

show abstract

“…Referral of suspected or known cases of CJD to the National Surveillance Teams of each country, which in the UK is in Edinburgh, is encouraged [35][36][37]. The latter centre can be consulted especially concerning the variant form of CJD.…”

Section: Prion Diseasesmentioning

confidence: 99%

Non-traumatic forensic neuropathology

Stewart¹,

Black²,

Kalimo³

et al. 2004

Forensic Science International

View full text Add to dashboard Cite

The ‘high‐risk’ neuropathological autopsy in AIDS and Creutzfeldt‐Jakob disease: principles and practice

Cited by 32 publications

References 18 publications

Simulated surgical-type cerebral biopsies from post-mortem brains allows accurate neuropathological diagnoses in the majority of neurodegenerative disease groups

Simulated surgical-type cerebral biopsies from post-mortem brains allows accurate neuropathological diagnoses in the majority of neurodegenerative disease groups

Standard Operating Procedures, ethical and legal regulations in BTB (Brain/Tissue/Bio) banking: what is still missing?

Non-traumatic forensic neuropathology

Contact Info

Product

Resources

About