The hisC1 gene, encoding aromatic amino acid aminotransferase-1 in Azospirillum brasilense Sp7, expressed in wheat

Castro-Guerrero, Julio; Palencia, Angélica Romero; Zúñiga-Aguilar, José Juan; Xiqui, Ma. Luisa; Sandoval, Jesús O.; Baca, Beatriz E.

doi:10.1007/s11104-011-1009-2

Cited by 15 publications

(5 citation statements)

References 44 publications

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“…The capability of A. brasilense Sp7 to affect the RLD and DCL after root application, confirmed the genomic evaluation performed and the presence of IPA pathway, despite of the invitro results. This was in accord with the scientific literature about the IAA-producing potential of strain A. brasilense Sp7 (Castro-Guerrero et al, 2012). For both K. variicola UC4115 and A. brasilense Sp7 we observed a negative tendency between biomass and RLD and DCL parameters.…”

Section: Seedling Assayssupporting

confidence: 92%

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Guerrieri¹,

Fiorini²,

Fanfoni³

et al. 2021

Front. Plant Sci.

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Plant growth promoting rhizobacteria (PGPR) can display several plant-beneficial properties, including support to plant nutrition, regulation of plant growth, and biocontrol of pests. Mechanisms behind these effects are directly related to the presence and expression of specific genes, and different PGPR strains can be differentiated by the presence of different genes. In this study we reported a comprehensive evaluation of a novel PGPR Klebsiella variicola UC4115 from the field to the lab, and from the lab to the plant. The isolate from tomato field was screened in-vitro for different activities related to plant nutrition and growth regulation as well as for antifungal traits. We performed a functional annotation of genes contributing to plant-beneficial functions previously tested in-vitro. Furthermore, the in-vitro characterization, the whole genome sequencing and annotation of K. variicola UC4115, were compared with the well-known PGPR Azospirillum brasilense strain Sp7. This novel comparative analysis revealed different accumulation of plant-beneficial functions contributing genes, and the presence of different genes that accomplished the same functions. Greenhouse assays on tomato seedlings from BBCH 11–12 to BBCH > 14 were performed under either organic or conventional management. In each of them, three PGPR inoculations (control, K. variicola UC4115, A. brasilense Sp7) were applied at either seed-, root-, and seed plus root level. Results confirmed the PGP potential of K. variicola UC4115; in particular, its high value potential as indole-3-acetic acid producer was observed in increasing of root length density and diameter class length parameters. While, in general, A. brasilense Sp7 had a greater effect on biomass, probably due to its high ability as nitrogen-fixing bacteria. For K. variicola UC4115, the most consistent data were noticed under organic management, with application at seed level. While, A. brasilense Sp7 showed the greatest performance under conventional management. Our data highlight the necessity to tailor the selected PGPR, with the mode of inoculation and the crop-soil combination.

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Section: Seedling Assayssupporting

confidence: 92%

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Guerrieri¹,

Fiorini²,

Fanfoni³

et al. 2021

Front. Plant Sci.

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“…These steps are catalyzed by enzymes, respectively aromatic aminotransferases and NAD-dependent aldehyde dehydrogenase, which are common and non-specific enzymes. Recently, the contribution of hisC1 , which encodes an aromatic amino acid aminotransferase-1 (AAT1), to IAA production was evidenced in A. brasilense Sp7 [ 40 ]. Homologues sharing identity levels higher than 75% with AAT1 from Sp7 were found in all four Azospirillum genomes (AZOLI_1579, AZCBG_330158, AZOBR_120044, AZL_012940).…”

Section: Resultsmentioning

confidence: 99%

Genome Sequence of Azospirillum brasilense CBG497 and Comparative Analyses of Azospirillum Core and Accessory Genomes provide Insight into Niche Adaptation

Wisniewski‐Dyé

Lozano

Acosta-Cruz

et al. 2012

Genes

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Bacteria of the genus Azospirillum colonize roots of important cereals and grasses, and promote plant growth by several mechanisms, notably phytohormone synthesis. The genomes of several Azospirillum strains belonging to different species, isolated from various host plants and locations, were recently sequenced and published. In this study, an additional genome of an A. brasilense strain, isolated from maize grown on an alkaline soil in the northeast of Mexico, strain CBG497, was obtained. Comparative genomic analyses were performed on this new genome and three other genomes (A. brasilense Sp245, A. lipoferum 4B and Azospirillum sp. B510). The Azospirillum core genome was established and consists of 2,328 proteins, representing between 30% to 38% of the total encoded proteins within a genome. It is mainly chromosomally-encoded and contains 74% of genes of ancestral origin shared with some aquatic relatives. The non-ancestral part of the core genome is enriched in genes involved in signal transduction, in transport and in metabolism of carbohydrates and amino-acids, and in surface properties features linked to adaptation in fluctuating environments, such as soil and rhizosphere. Many genes involved in colonization of plant roots, plant-growth promotion (such as those involved in phytohormone biosynthesis), and properties involved in rhizosphere adaptation (such as catabolism of phenolic compounds, uptake of iron) are restricted to a particular strain and/or species, strongly suggesting niche-specific adaptation.

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“…In Chromobacterium violaceum it has been proved that indole-3-carboxylic acid is a degradation product of indole-3-acetic acid, which is produced from L-tryptophan [33] (Figure 4). The biosynthesis of indol-3-acetic acid from L-tryptophan has been demonstrated in different microorganisms [37,51]. In S. espanaensis we postulate that Ltryptophan is even the starting molecule for indole-3-carboxylic acid formation.…”

Section: Discussionmentioning

confidence: 81%

Insight into Saccharothrix espanaensis Grown in Different Media:Transcriptome and Natural Product Analysis

Samra¹,

Tokovenko²,

Kaszubowska³

et al. 2018

Nat Prod Chem Res

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In this study, we show that cultivating of Saccharothrix espanaensis in the amino acid-rich SPY medium led to the detection of antibacterial and antifungal products. The cultivation in GYM-, HA-or NL111-medium did not lead to the production of these compounds. Four products were isolated from Saccharothrix espanaensis-SPY culture and determined as the known compounds anthranilic acid, indol-3-carboxylic acid, benzoic acid, and phenylacetic acid. In order to investigate the influence of SPY medium on gene transcription and to identify candidate genes involved in the biosynthesis of the purified compounds a comparative RNA sequencing analysis of Saccharothrix espanaensis cultivated in different media was performed. Total transcriptome analysis demonstrated that media composition has an influence on the expression of several genes. Among them are genes encoding enzymes belong to amino acid metabolism enzymes. Furthermore, based on the collected data genes involved in the biosynthesis of anthranilic acid, indol-3-carboxylic acid, benzoic acid, and phenylacetic acid could be predicted. Deletion of the genes BN6_01860 and BN6_67950 revealed their importance in the biosynthesis of indole-3carboxylic acid and benzoic acid, respectively.

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The hisC1 gene, encoding aromatic amino acid aminotransferase-1 in Azospirillum brasilense Sp7, expressed in wheat

Cited by 15 publications

References 44 publications

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Genome Sequence of Azospirillum brasilense CBG497 and Comparative Analyses of Azospirillum Core and Accessory Genomes provide Insight into Niche Adaptation

Insight into Saccharothrix espanaensis Grown in Different Media:Transcriptome and Natural Product Analysis

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