The histomorphometric parameters of endocrine pancreas after bariatric surgery in healthy animal models

Camacho-Ramírez, Alonso; Gomar, David Almorza; Díaz-Gómez, Alfredo; Mateo-Gavira, Isabel; Macías-Rodríguez, M; Pérez-Arana, Gonzalo M; Oliveira, José Arturo Prada

doi:10.1016/j.tice.2019.02.008

Cited by 4 publications

(7 citation statements)

References 29 publications

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“…11 In addition to alteration of beta-cell function, bariatric surgery appears to modify some histo-morphometric parameters of islets, such as the number of beta cells per islet. 10,12 In healthy Wistar rats, such effect is exclusively related to increased proliferation and maturation of beta-cells from stem cells, which is consistent with the islet regeneration described in db/db mice after gastric bypass through the PDX-1/Notch-1/Ngn3 signalling. 13 On the other hand, in diabetic GK rats, multiple and bigger effects on islet architecture have been reported, including pancreatic hyperplasia, enlarged beta cell-mass, and increased ratio of beta cells to non-beta endocrine cells.…”

Section: Improvement In Islet Function Is Crucial For Diabetes Remisssupporting

confidence: 73%

PYY, a Therapeutic Option for Type 2 Diabetes?

Guida

Ramracheya

2020

Clin Med Insights Endocrinol Diabetes

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Metabolic surgery leads to rapid and effective diabetes reversal in humans, by weight-independent mechanisms. The crucial improvement in pancreatic islet function observed after surgery is induced by alteration in several factors, including gut hormones. In addition to glucagon-like peptide 1 (GLP-1), increasing lines of evidence show that peptide tyrosine tyrosine (PYY) plays a key role in the metabolic benefits associated with the surgery, ranging from appetite regulation to amelioration of islet secretory properties and survival. Here, we summarize the current knowledge and the latest advancements in the field, which pitch a strong case for the development of novel PYY-based therapy for the treatment of diabetes.

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Section: Improvement In Islet Function Is Crucial For Diabetes Remisssupporting

confidence: 73%

PYY, a Therapeutic Option for Type 2 Diabetes?

Guida

Ramracheya

2020

Clin Med Insights Endocrinol Diabetes

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“…The remnant gastric fundus was anastomosed to the jejunum at 14 cm distal from the ligament of Treitz. The abdominal muscular and skin layers were closed in one layer using a continuous suturing technique [17,18].…”

Section: Surgical Proceduresmentioning

confidence: 99%

The Leading Role of Peptide Tyrosine Tyrosine in Glycemic Control After Roux-en-Y Gastric Bypass in Rats

et al. 2019

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Aims Roux-en-Y gastric bypass (RYGB) is one of the most effective surgical therapies for the rapid resolution of type 2 diabetes. However, the mechanisms underlying the entero-hormonal response after surgery and the role of peptide tyrosine tyrosine (PYY) in the restoration of normoglycemia are still not clear. Methods We reproduced the RYGB technique in Wistar and Goto-Kakizaki rats and performed serum hormonal, histological, and hormonal-infusion test. Results Using the diabetic Goto-Kakizaki (GK) rat model, we demonstrated that PYY plasma levels showed a remarkable peak approximately 30 min earlier than GLP-1 or GIP after mixed-meal administration in RYGB-operated rats with PYY. The GLP-1 and GIP areas under the curve (AUCs) increased after RYGB in GK rats. Additionally, the findings suggested that PYY (3-36) infusion led to increased GLP-1 and GIP plasma levels close to those obtained after a meal. Finally, the number of GLP-1positive cells appeared to increase in the three segments of the small intestine in GK-RYGB-operated rats beyond the early presence of nutrient stimulation in the ileum. Nevertheless, PYY-positive cell numbers appeared to increase only in the ileum. Conclusion At least in rats, these data demonstrate an earlier essential role for PYY in gut hormone regulation after RYGB. We understand that PYY contributes to GLP-1 and GIP release and there must be the existence of enteroendocrine communication routes between the distal and proximal small intestine.

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“…Instead of this, this is an malabsorptive intestinal massive resection model, in which we study the cellularity consequences on beta-cell mass. Some reports had published changes in endocrine pancreas cellularity, with important differences between restrictive versus malabsorptive techniques 9 . However, no much consideration had received the analysis of the cellular changes.…”

Section: Discussionmentioning

confidence: 99%

“…Similar cellular changes have been reported in endocrine pancreatic cells after bariatric surgeries in healthy animal models. These reports included the histomorphometric analysis of the pancreatic islets with significant differences according to the surgical aggression on the digestive tube 9,10 . Now, our aim is to study the histological consequences on the islet conformation after a severe malabsorptive surgery in diabetic rats.…”

Section: Introductionmentioning

confidence: 99%

La influencia de las resecciones masivas intestinales sobre el páncreas endocrino de ratas diabéticas Goto-Kakizaki

Camacho-Ramírez¹,

Mayo-Ossorio²,

Pacheco-García³

et al. 2022

CIRU

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Objective: Several bariatric surgeries have been related to the T2DM improvement in obese patients. Despite the different mechanism invoked for this improvement, many evidences showed that the pancreas cellularity is conditioned for the homeostatic physiological changes after these surgeries. Many authors reported the changes in beta-cell mass after some surgeries in healthy rats. We purpose to analyze the changes in b-cell cellularity and b-cell-mass after a severe malabsorptive surgical method. Thus, we studied several parameters of the islet morphometric composition after a massive jejunal resection. Materials and methods: We employed Goto-Kakizaki diabetic non-obese rats, which underwent the 50% resection of middle portion of the jejunum versus a control group. After 3 months, rats were sacrificed and pancreas was immunohistochemicaly studied. Results: The b-cell mass was analyzed and several parameters about the endocrine islet size distribution were studied. We report an increase of b-cell mass in massive resection surgical group versus controls. The islet distribution was significant different between both groups. Endocrine islets of surgical group were bigger with a different cellular distribution. Conclusion: According to the enteroendocrine changes related to surgeries in jejunum, as in other gastrointestinal portions, the cellularity of islets changes as an adaptive process to glycemic demands.

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The histomorphometric parameters of endocrine pancreas after bariatric surgery in healthy animal models

Cited by 4 publications

References 29 publications

PYY, a Therapeutic Option for Type 2 Diabetes?

PYY, a Therapeutic Option for Type 2 Diabetes?

The Leading Role of Peptide Tyrosine Tyrosine in Glycemic Control After Roux-en-Y Gastric Bypass in Rats

La influencia de las resecciones masivas intestinales sobre el páncreas endocrino de ratas diabéticas Goto-Kakizaki

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