The histone acetyltransferase PsGcn5 mediates oxidative stress responses and is required for full virulence of Phytophthora sojae

Zhao, Wei; Wang, Tao; Liu, Shusen; Chen, Qingqing; Ran, Qi

doi:10.1016/j.micpath.2015.07.015

Cited by 15 publications

(22 citation statements)

References 46 publications

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“…However, the growth rate of the S. cerevisiae gcn5 null mutant does not differ from that of the WT strain, except under conditions of limited NH 4 NO 3 concentrations (Georgakopoulos and Thireos, 1992). In contrast to our results, the Phytophthora sojae gcn5 mutant develops normally, but is hypersensitive to hydrogen peroxide (Zhao et al, 2015). These observations imply that Gcn5 regulates morphogenesis in a fungal species-specific manner.…”

Section: Discussioncontrasting

confidence: 99%

“…This is consistent with the fact C. albicans Gcn5 is not involved in oxidative stress responses (Chang et al, 2015). However, some fungal species are more sensitive to oxidative stress than A. flavus , including C. neoformans (O’Meara et al, 2010) and P. sojae (Zhao et al, 2015). As a core subunit of the SAGA complex, Gcn5 affects the yeast HOG pathway (Spedale et al, 2012), but Hog1 kinase does not appear to be involved in A. flavus responses to oxidative and osmotic stresses (Baidya et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, Gcn5 is required for the pathogenesis of C. albicans in a mouse model (Chang et al, 2015). Similarly, among plant pathogens, the U. maydis gcn5 mutant is avirulent to maize plants (González-Prieto et al, 2014), while P. sojae Gcn5 is required for full virulence during infections of soybean (Zhao et al, 2015). We observed that the ability of the Δ AflgcnE mutant to infect maize seeds is inhibited ( Figure 8 ), confirming the importance of Gcn5 for the pathogenicity of this species.…”

Section: Discussionmentioning

confidence: 99%

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The Aspergillus flavus Histone Acetyltransferase AflGcnE Regulates Morphogenesis, Aflatoxin Biosynthesis, and Pathogenicity

et al. 2016

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Histone acetyltransferases (HATs) help regulate fungal development and the production of secondary metabolites. In this study, we determined that the HAT AflGcnE influenced morphogenesis and aflatoxin biosynthesis in Aspergillus flavus. We observed that AflGcnE localized to the nucleus and cytoplasm during the conidial production and germination stages, while it was located mainly in the nucleus during the hyphal development stage. Deletion of AflgcnE inhibited the growth of A. flavus and decreased the hydrophobicity of the cell surface. The ΔAflgcnE mutant exhibited a lack of asexual sporulation and was unable to generate sclerotia. Additionally, AflgcnE was required to maintain cell wall integrity and genotoxic stress responses. Importantly, the ΔAflgcnE mutant did not produce aflatoxins, which was consistent with a significant down-regulation of aflatoxin gene expression levels. Furthermore, our data revealed that AflgcnE is a pathogenicity factor required for colonizing maize seeds. In summary, we revealed that A. flavus AflGcnE is crucial for morphological development, aflatoxin biosynthesis, stress responses, and pathogenicity. Our findings help clarify the functional divergence of GcnE orthologs, and may provide a possible target for controlling A. flavus infections of agriculturally important crops.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Aspergillus flavus Histone Acetyltransferase AflGcnE Regulates Morphogenesis, Aflatoxin Biosynthesis, and Pathogenicity

et al. 2016

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“…Previous work suggested that the heat shock transcription factor PsHSF1 and histone acetyltransferase PsGcn5 were involved in oxidative stress responses and detoxifying oxidative bursts (Sheng et al, 2015 ; Zhao et al, 2015 ). Our study showed that PiLLP is involved in the H 2 O 2 resistance and ROS scavenging, which are likely caused by affecting the transcription level of catalase genes.…”

Section: Discussionmentioning

confidence: 99%

The Loricrin-Like Protein (LLP) of Phytophthora infestans Is Required for Oospore Formation and Plant Infection

et al. 2017

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Loricrin-like protein (LLP) is characterized by a high content of glycine residues and is a major component of plant cell wall. Here, we identified a Phytophthora infestans ortholog of plant LLP, named PiLLP. In P. infestans, PiLLP is strongly expressed in asexual and sexual developmental stages, including in sporangia, zoospores and germinating cysts, and during oospore formation, as well as in the early stages of infection and during hydrogen peroxide stress. Compared with the wild type, the PiLLP-silenced transformants were defective in oospore formation, had slower colony expansion rates, produced less sporangia with lower germination and zoospore-release rates, and were more sensitive to hydrogen peroxide. Moreover, Nile red staining, and PiLLP-red fluorescent protein fusions indicated that PiLLP is involved in oogonia formation. The silenced transformants also had severely diminished virulence levels that could be partially restored with diphenyleneiodium treatments. The analysis of catalase activity showed a decrease of catalase activity in silenced transformants. Thus, PiLLP is important for sexual and asexual reproduction, and is required for oxidative stress tolerance and plant infection.

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“…In B. bassiana , deletion of g cn5 led to severe defects in colony growth and loss of cuticle infection ( Cai et al, 2018b ). In P. sojae , although Δ gcn5 mutants had a normal development, their virulence in soybean was significantly reduced ( Zhao et al, 2015 ). KDACs are also necessary for fungal pathogenesis, which were found to be decisive regulators of genes involved in pathogenicity and fungal toxin production, regulating a number of physiological processes, including thermotolerance, capsule formation, melanin synthesis, protease activity, and cell wall integrity ( Bauer et al, 2016 , 2019 ; Brandão et al, 2018 ).…”

Section: Role Of Acetylation/deacetylation In Fungal Virulencementioning

confidence: 99%

Protein Acetylation/Deacetylation: A Potential Strategy for Fungal Infection Control

et al. 2020

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Protein acetylation is a universal post-translational modification that fine-tunes the major cellular processes of many life forms. Although the mechanisms regulating protein acetylation have not been fully elucidated, this modification is finely tuned by both enzymatic and non-enzymatic mechanisms. Protein deacetylation is the reverse process of acetylation and is mediated by deacetylases. Together, protein acetylation and deacetylation constitute a reversible regulatory protein acetylation network. The recent application of mass spectrometry-based proteomics has led to accumulating evidence indicating that reversible protein acetylation may be related to fungal virulence because a substantial amount of virulence factors are acetylated. Additionally, the relationship between protein acetylation/deacetylation and fungal drug resistance has also been proven and the potential of deacetylase inhibitors as an anti-infective treatment has attracted attention. This review aimed to summarize the research progress in understanding fungal protein acetylation/deacetylation and discuss the mechanism of its mediation in fungal virulence, providing novel targets for the treatment of fungal infection.

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The histone acetyltransferase PsGcn5 mediates oxidative stress responses and is required for full virulence of Phytophthora sojae

Cited by 15 publications

References 46 publications

The Aspergillus flavus Histone Acetyltransferase AflGcnE Regulates Morphogenesis, Aflatoxin Biosynthesis, and Pathogenicity

The Aspergillus flavus Histone Acetyltransferase AflGcnE Regulates Morphogenesis, Aflatoxin Biosynthesis, and Pathogenicity

The Loricrin-Like Protein (LLP) of Phytophthora infestans Is Required for Oospore Formation and Plant Infection

Protein Acetylation/Deacetylation: A Potential Strategy for Fungal Infection Control

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