The Hormonal Control of Implantation

Aitken, R. John

doi:10.1002/9780470720479.ch4

Cited by 14 publications

(2 citation statements)

References 110 publications

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“…After ovulation, all circulating P is produced by the corpus luteum and if pregnancy occurs, this production continues until the 7 th week when there is a shift and the placenta takes over. P is vital for the development and maturation of the endometrium and subsequently for the implantation of the embryo (Aitken, 1978).…”

Section: Hormonal Regulation Of Endometrial Receptivitymentioning

confidence: 99%

Polyethylene glycated leukemia inhibitory factor antagonist inhibits human blastocyst implantation and triggers apoptosis by down-regulating embryonic AKT

Lalitkumar

Boggavarapu

Menezes

et al. 2013

Fertility and Sterility

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Section: Hormonal Regulation Of Endometrial Receptivitymentioning

confidence: 99%

Polyethylene glycated leukemia inhibitory factor antagonist inhibits human blastocyst implantation and triggers apoptosis by down-regulating embryonic AKT

Lalitkumar

Boggavarapu

Menezes

et al. 2013

Fertility and Sterility

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“…Progesterone can help to prepare the uterus for implantation and development of a fertilized egg (Aitken 1978). Estrogen is a critical determinant that specifies the duration of the window of uterine receptivity for implantation (Ma et al 2003).…”

Section: The Expression Of Grb10 In Rat Uterusmentioning

confidence: 99%

MiR199a is implicated in embryo implantation by regulating Grb10 in rat

Xia¹,

Cao²,

Jin³

et al. 2014

Reproduction

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MiR199a was found to be differentially expressed in rat uteri between the prereceptive and receptive phase via microRNA (miRNA) microarray analysis in our previous study. However, the role of miR199a in rat embryo implantation remained unknown. In the study, northern blot results showed that the expression levels of miR199a were higher on gestation days 5 and 6 (g.d.5-6) in rat uteri than on g.d.3-4 and g.d.7-8. In situ localization of miR199a in rat uteri showed that miR199a was mainly localized in the stroma or decidua. The expression of miR199a was not significantly different in the uteri of pseudopregnant rats and evidently increased in the uteri of rats subjected to activation of delayed implantation and experimentally induced decidualization. Treatment with 17b-estradiol or both 17b-estradiol and progesterone significantly diminished miR199a levels. Gain of function of miR199a in endometrial stromal cells isolated from rat uteri inhibited cell proliferation and promoted cell apoptosis. Loss of function of miR199a displayed opposite roles on cell proliferation and apoptosis. Further investigation uncovered a significant inverse association between the expression of miR199a and growth factor receptor-bound protein 10 (Grb10), an imprinted gene, and miR199a could bind to the 3 0 UTR of Grb10 to inhibit Grb10 translation. In addition, in vivo analysis found that the immunostaining of GRB10 was attenuated in the stroma or decidua from g.d.4 to 6, contrary to the enhancement of miR199a. Collectively, upregulation of miR199a in rat uterus during the receptive phase is regulated by blastocyst activation and uterine decidualization. Enforced miR199a expression suppresses cell proliferation partially through targeting Grb10.Reproduction ( Although a large number of studies have been conducted to explore the exact molecular mechanisms of embryo implantation, there are many unanswered questions about the specific mechanisms associated with the onset of uterine receptivity and embryo implantation. MicroRNAs (miRNAs) are short noncoding RNAs that are involved in several important biological processes through the regulation of gene expression at the posttranscriptional level (Pan et al. 2013. The miRNAs elicit their effects by binding to the 3 0 UTR of their target mRNAs, leading to the inhibition of translation or the degradation of the mRNA, depending on the degree of complementary base pairing (Guo et al. 2013, Han et al. 2013, Wang et al. 2013a. Our previous study found that miR199a was differentially expressed in rat uteri between the prereceptive and receptive phase by miRNA microarray analysis. MiR199a* was spatiotemporally expressed in mouse uterus during embryo implantation (Chakrabarty et al. 2007). However, the expression and function of miR199a in embryo implantation in rats is still unknown.In this study, we report the spatiotemporal profiles of miR199a in rat uterus during peri-implantation and the effects of pseudopregnancy, artificial decidualization, activation of delayed implantation, and hormon...

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Biochemical aspects of conceptus–endometrial interactions

Roberts

1983

J. Exp. Zool.

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Mammalian conceptuses must provide a chemical signal to the maternal system to insure maintenance of corpus luteum (CL) function and of progesterone production and continuation of uterine endometrial secretory activity. These events insure that the developing conceptus is provided with appropriate nutrients, regulatory enzymes and endocrine state to allow successful establishment and maintenance of pregnancy. Pig blastocysts begin to produce estrogens by Day 11 of pregnancy, which prevents secretion of the uterine luteolytic factor (PGF2 alpha) in an endocrine direction, but allows secretion in an exocrine direction, i.e., into the uterine lumen. Therefore, CL are "protected." Blastocyst estrogens also trigger secretion of a group of proteins, including uteroferrin, an iron transport protein, and a family of protease inhibitors whose biosynthesis within the uterine glandular epithelium is under the control of progesterone. Estrogen also appears to promote accumulation of glucose and fructose within the uterine lumen. A complex in vivo "culture medium" is thereby established to promote conceptus development. Pig blastocysts do not undergo invasive implantation within the uterine lumen although they produce the protease, plasminogen activator. Invasion may be prevented by endometrial secretion of progesterone-induced protease inhibitors which are produced in large amounts. In addition to estrogens of conceptus origin, calcium and prostaglandins PGF2 alpha and E2 may affect the uterine vasculature, water and electrolyte transport, capillary permeability, conceptus steroid production, and related events during pregnancy. The blastocysts of the large domestic animals also secrete proteins which include a large glycoprotein (Mr approximately 600,000) and a small acidic protein (Mr approximately 17,000). The latter has been purified from sheep and named ovine trophoblast protein I. These proteins may play unique roles in early pregnancy with respect to establishment and maintenance of pregnancy in the ewe, sow, mare, and cow.

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The Hormonal Control of Implantation

Cited by 14 publications

References 110 publications

Polyethylene glycated leukemia inhibitory factor antagonist inhibits human blastocyst implantation and triggers apoptosis by down-regulating embryonic AKT

Polyethylene glycated leukemia inhibitory factor antagonist inhibits human blastocyst implantation and triggers apoptosis by down-regulating embryonic AKT

MiR199a is implicated in embryo implantation by regulating Grb10 in rat

Biochemical aspects of conceptus–endometrial interactions

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