The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5

Kropinski, Andrew M.; Waddell, Tom; Meng, Juncai; Franklin, Kristyn; Ackermann, Hans-Wolfgang; Ahmed, Rafiq; Mazzocco, Amanda; Yates, John R.; Lingohr, Erika J.; Johnson, Roger P.

doi:10.1186/1743-422x-10-76

Cited by 62 publications

(48 citation statements)

References 86 publications

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“…Five to 6 thin tail fibers of 61 nm in length were occasionally seen. The morphology of AXO121A closely resembles that reported for phage rV5, in which the head measured 91 nm in diameter and the contractile tail was 121 nm by 17 nm with 70 nm tail fibers (Kropinski et al 2013). Phages AYO26A and vB_EocM_AXO121B had a large elongated head with a diameter of 115-117 nm.…”

Section: Phage Morphologysupporting

confidence: 74%

Feces of feedlot cattle contain a diversity of bacteriophages that lyse non-O157 Shiga toxin-producing Escherichia coli

et al. 2015

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This study aimed to isolate and characterize bacteriophages that lyse non-O157 Shiga toxin-producing Escherichia coli (STEC) from cattle feces. Of 37 non-O157 STEC-infecting phages isolated, those targeting O26 (AXO26A, AYO26A, AYO26B), O103 (AXO103A, AYO103A), O111 (AXO111A, AYO111A), O121 (AXO121A, AXO121B), and O145 (AYO145A, AYO145B) were further characterized. Transmission electron microscopy showed that the 11 isolates belonged to 3 families and 6 genera: the families Myoviridae (types rV5, T4, ViI, O1), Siphoviridae (type T5), and Podoviridae (type T7). Genome size of the phages as determined by pulsed-field gel electrophoresis ranged from 38 to 177 kb. Excluding phages AXO26A, AYO103A, AYO145A, and AYO145B, all other phages were capable of lysing more than 1 clinically important strain from serogroups of O26, O91, O103, O111, O113, O121, and O128, but none exhibited infectivity across all serogroups. Moreover, phages AYO26A, AXO121A, and AXO121B were also able to lyse 4 common phage types of STEC O157:H7. Our findings show that a diversity of non-O157 STEC-infecting phages are harbored in bovine feces. Phages AYO26A, AYO26B, AXO103A, AXO111A, AYO111A, AXO121A, and AXO121B exhibited a broad host range against a number of serogroups of STEC and have potential for the biocontrol of STEC in the environment.

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Section: Phage Morphologysupporting

confidence: 74%

Feces of feedlot cattle contain a diversity of bacteriophages that lyse non-O157 Shiga toxin-producing Escherichia coli

et al. 2015

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“…The host range of phage Bp7 for 35 clinical E. coli strains and 4 laboratory E. coli strains was determined by spotting 10 5 PFU of Bp7 onto freshly seeded lawns of bacteria on agar plates (13). Each test was repeated three times.…”

Section: Methodsmentioning

confidence: 99%

T4-Like Phage Bp7, a Potential Antimicrobial Agent for Controlling Drug-Resistant Escherichia coli in Chickens

Zhang

Liu

et al. 2013

Appl Environ Microbiol

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Chicken-pathogenic Escherichia coli is severely endangering the poultry industry in China and worldwide, and antibiotic therapy is facing an increasing problem of antibiotic resistance. Bacteriophages can kill bacteria with no known activity in human or animal cells, making them an attractive alternative to antibiotics. In this study, we present the characteristics of a novel virulent bacteriophage, Bp7, specifically infecting pathogenic multidrug-resistant E. coli. Phage Bp7 was isolated from chicken feces. Bp7 belongs to the family Myoviridae, possessing an elongated icosahedral head and contractile sheathed tail. It has a 168-kb doublestranded DNA genome. For larger yields, its optimal multiplicity of infection (MOI) to infect E. coli was about 0.001. The latent period was 10 to 15 min, and the burst size was 90 PFU/infected cell. It was stable both at pH 5.0 to 10.0 and at 40°C or 50°C for at least 1 h. Bp7 could infect 46% of pathogenic clinical E. coli strains. Bp7 harbored 791 open reading frames (ORFs) and 263 possible genes. Among the 263 genes, 199 possessed amino acid sequence identities with ORFs of phage T4, 62 had identities with other T4-like phages, and only one lacked any database match. The genome of Bp7 manifested obvious division and rearrangement compared to phages T4, JS98, and IME08. Bp7 is a new member of the "T4-like" genus, family Myoviridae. Its wide host range, strong cell-killing activity, and high stability to pH make it an alternative to antimicrobials for controlling drug-resistant E. coli in chickens. Chicken colibacillosis is one of the main bacterial diseases and severely endangers the poultry industry in China and worldwide. Escherichia coli has been identified as a major pathogen (1). Antibiotics are widely used to control chicken colibacillosis, but it is very common for E. coli to be resistant to antibiotics (2, 3). In recent years, nearly 80% of E. coli isolates from diseased animals have manifested severe resistance to antimicrobial drugs (4, 5), so antibiotic therapy is facing an increasing problem of antibiotic resistance. Bacteriophages are now considered a good alternative to antibiotics (6, 7).However, there are many problems with phage therapy, and not every phage strain is appropriate for such therapy. Based upon their replication methods, phages are classified as either virulent or lysogenic. Virulent phages replicate in their bacterial hosts and destroy them in the process, but lysogenic phages insert their genomes into their hosts' genomes (8). As it has turned out, both lysogenic and virulent bacteriophages are actively involved in the evolution of bacteria, including pathogens (9). A troubling possibility is that there are virulence genes in some phages and these genes can change the pathogenicity of their host bacteria. Lysogenic phages transfer genes that express toxin proteins or pathogenic factors among bacterial species (8, 10). For safety reasons, lysogenic phages are not allowed to be used in phage therapy, and if a phage is permitted to be an alternati...

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“…They share (1) genome sizes ranging from 136 kb (phage vB_ EcoM-FV3) to 148 kb (phage phi92), (2) similar number of protein coding sequences (CDSs) ranging from 233 (rV5) to 269 (vB_ CsaM_GAP31), (3) mol.% G + C ranging from 37.4 % (phi92) to 46.3 % (vB_CsaM_GAP31), and (4) number of tRNAs ranging from fi ve (vB_EcoM-FV3) to 26 (vB_CsaM_GAP31) [ 36 -41 ]. Recently, doubt has been cast on whether this grouping represents a genus or subfamily ("V5virinae") containing three genera-the "V5likevirus" (rV5, FV3), the "Pvplikevirus" (PVP-SE1, GAP31, and SSE-121), and the "Phi92likevirus" (phi92 and phAPEC8) [ 37 ].…”

Section: Felixounalikevirusmentioning

confidence: 99%

Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

Switt

Sulakvelidze

Wiedmann

et al. 2014

Methods in Molecular Biology

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Since this book was originally published in 2007 there has been a significant increase in the number of Salmonella bacteriophages, particularly lytic virus, and Salmonella strains which have been fully sequenced. In addition, new insights into phage taxonomy have resulted in new phage genera, some of which have been recognized by the International Committee of Taxonomy of Viruses (ICTV). The properties of each of these genera are discussed, along with the role of phage as agents of genetic exchange, as therapeutic agents, and their involvement in phage typing.

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The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5

Cited by 62 publications

References 86 publications

Feces of feedlot cattle contain a diversity of bacteriophages that lyse non-O157 Shiga toxin-producing Escherichia coli

Feces of feedlot cattle contain a diversity of bacteriophages that lyse non-O157 Shiga toxin-producing Escherichia coli

T4-Like Phage Bp7, a Potential Antimicrobial Agent for Controlling Drug-Resistant Escherichia coli in Chickens

Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

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