The host range of <i>Tobacco streak virus</i> in India and transmission by thrips

Rao, R. D. V. J. Prasada; Reddy, A. G. S.; Reddy, S. V.; Thirumala‐Devi, K.; Rao, S. Chander; Kumar, Vipin; Subramaniam, Kuttichantran; Reddy, T. Yellamanda; Nigam, S. N.; Reddy, D. V. R.

doi:10.1111/j.1744-7348.2003.tb00262.x

Cited by 85 publications

(57 citation statements)

References 7 publications

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“…This type of results was supported by Bhaskara Reddy et al (2012) who raised the polyclonal antibody against the TSV and showed positive reaction for sample collected from natural infection of Hibiscus cannabinus in DAC-ELISA. Prasad Rao et al (2003) also proved the detection of a new strain SB-10 of TSV from potato through DAC-ELISA. In our study virus preparation has reacted with the antiserum specific to TSV, which was produced approximately 29 kDa protein in western blot assay.…”

Section: Discussionmentioning

confidence: 99%

“…TSV can be transmitted mechanically, but the transmission of TSV commonly occurs through different species of thrips viz., Megalurothrips usitatus, Frankliniella schultzei, Scirtothrips dorsalis, Thrips palmi and Thrips tabaci under field conditions (Jagtap et al, 2012) Alternative host plants have been suspected to harbour TSV which have contributed in its transmission. The virus causes asymptomatic infections in several common weed species, including Parthenium hysterophorus, Ageratum conyzoides and Corchorus trilocularis, whose pollen is a major source of TSV and these plants, also harbour thrips (Prasada Rao et al, 2003;Shukla et al, 2005). Though the occurrence of TSV has been reported from many hosts in India, only limited reports are available on the biological and molecular characterization of these isolates and their exact identification remains unaddressed in soybean.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and genetic diversity of Tobacco streak virus infecting soybean (Glycine max L.)

Rajamanickam¹,

Karthikeyan²

2016

Afr. J. Microbiol. Res.

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Soybean (Glycine max L.), the most popular oil seed crop, grown in the experimental fields of Tamil Nadu Agricultural University and adjoining areas exhibited symptoms of necrosis and stunting. The symptoms were similar to bud blight of soybean caused by the Tobacco streak virus (TSV) and the disease is most prevalent throughout the country. To study the nature of infection, sap inoculation of the soybean strain induced local as well systemic infection on cowpea plants cv. C 152 and resulted in the production of circular necrotic lesions and death of plants. The samples were also serologically positive in DAC-ELISA and it has also yielded a protein band of approximately 29 kDa corresponding to coat protein of TSV in Western blot assay. For the characterization of virus, RT-PCR was carried out with a newly designed coat protein gene specific primer, which resulted in amplification of the expected 929 bp size. Sequence analysis of the CP gene had nucleotide similarity of 80.6 to 99.3% with known isolates of TSV. The multiple sequence alignment revealed that CP gene showed single unique variation and some of deletion and addition mutation was found in nucleotide and amino acid sequences against the isolates of other soybean Brazil and USA isolates and produced single unique variation at position 344 where adenine was substituted with guanine. There was no deletion and addition between nucleotide sequences in the group of Indian isolates, further confirms the placement of the soybean isolate of TSV in a single subgroup.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular characterization and genetic diversity of Tobacco streak virus infecting soybean (Glycine max L.)

Rajamanickam¹,

Karthikeyan²

2016

Afr. J. Microbiol. Res.

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“…dorsalis is also mentioned as a vector of the chilli leaf curl virus (CLCV) and tobacco streak virus (TSV) (Prasada Rao et al, 2003).…”

Section: 5mentioning

confidence: 99%

Scientific Opinion on the pest categorisation of Scirtothrips dorsalis

Rossi¹

2014

EFS2

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The EFSA Panel on Plant Health undertook a pest categorisation for the insect Scirtothrips dorsalis for the European Union. Although there are reports that this is a species complex and there are host-specific races, it is generally treated as a distinct taxon. Both morphological and molecular methods are required to confirm identification. Apart from one long-term outbreak in a botanic garden glasshouse in England, it is absent from the EU. Native to southern and eastern Asia, this species has been introduced to tropical and subtropical areas of Africa, Australasia and the Americas in recent years. It is highly polyphagous, with over 225 known hosts, which include many important EU crops. Southern areas of the EU are potentially suitable for outdoor establishment and it could establish in protected cultivation throughout the EU. Based on its phenology, the Panel showed that the climate in southern Europe could allow a similar number of generations to develop as in Japan and South Korea, where significant damage occurs to citrus and other crops outdoors. In protected cultivation, even though control methods used against other thrips species may be effective in keeping populations at low densities, damage can still occur at these densities owing to the transmission of tospoviruses. Despite being highly polyphagous, S. dorsalis is an insect listed in Annex IIAI of Council Directive 2000/29/EC only in relation to Citrus, Fortunella and Poncirus plants. These hosts are also regulated in Annex III and Annex V. They are also explicitly mentioned in Council Directive 2008/90/EC.

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“…In India, TSV was first reported on sunflower (Bhat et al, 2002) and subsequently reported in several agricultural and horticultural crops (Prasada Rao et al, 2003;Vemana & Jain, 2010;Sivaprasad et al, 2010). TSV is pollen-borne and is easily spread by thrips under field conditions (Prasada Rao et al, 2003).…”

mentioning

confidence: 99%

“…TSV is pollen-borne and is easily spread by thrips under field conditions (Prasada Rao et al, 2003). TSV infection in jasmineis widespread in A.P.…”

mentioning

confidence: 99%