The hrpN gene of Erwinia amylovora stimulates tobacco growth and enhances resistance to Botrytis cinerea

Jang, Young-Sun; Sohn, Soo-In; Wang, Myeong-Hyeon

doi:10.1007/s00425-005-0100-4

Cited by 38 publications

(17 citation statements)

References 26 publications

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“…Although this interaction needs to be confirmed in vivo, these results are consistent with the hypothesis that FIB4 is an HrpN target given that reduction of FIB4 expression could amplify the impact of HrpN and lead to increased disease susceptibility (101). Surprisingly, HrpN is also reported to enhance plant growth (39,93). This effect is likely to be mediated by HIPM, a 6.5-kDa plasma membrane-localized protein that interacts with HrpN in yeast and in vitro and functions as a negative regulator of plant growth (79).…”

Section: Transgenic and Cisgenic Applicationssupporting

confidence: 87%

Fire Blight: Applied Genomic Insights of the Pathogen and Host

Malnoy

Martens

Norelli

et al. 2012

Annu. Rev. Phytopathol.

133

101

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The enterobacterial phytopathogen Erwinia amylovora causes fire blight, an invasive disease that threatens a wide range of commercial and ornamental Rosaceae host plants. The response elicited by E. amylovora in its host during disease development is similar to the hypersensitive reaction that typically leads to resistance in an incompatible host-pathogen interaction, yet no gene-for-gene resistance has been described for this host-pathogen system. Comparative genomic analysis has found an unprecedented degree of genetic uniformity among strains of E. amylovora, suggesting that the pathogen has undergone a recent genetic bottleneck. The genome of apple, an important host of E. amylovora, has been sequenced, creating new opportunities for the study of interactions between host and pathogen during fire blight development and for the identification of resistance genes. This review includes recent advances in the genomics of both host and pathogen.

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Section: Transgenic and Cisgenic Applicationssupporting

confidence: 87%

Fire Blight: Applied Genomic Insights of the Pathogen and Host

Malnoy

Martens

Norelli

et al. 2012

Annu. Rev. Phytopathol.

133

101

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“…Early blight is a major fungal disease in tomato and is caused by Alternaria solani. The pathogens used in the present study have been widely used in the disease resistance assay (Terakawa et al, 1997;Chakrabarti et al, 2003;Jang et al 2006). Transgenic plants with endochitiase gene from T. harzianum have shown enhanced resistance to B. cinerea and A. alternata (Lorito et al, 1998).…”

Section: Discussionmentioning

confidence: 89%

Expression of a fungal endochitinase gene in transgenic tomato and tobacco results in enhanced tolerance to fungal pathogens

Shah

Mukherjee

Eapen

2010

Physiol Mol Biol Plants

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Development of transgenic Nicotiana tabacum and Lycopersicon esculentum expressing an endochitinase (ech42) gene from biocontrol fungus Trichoderma virens using Agrobacterium-mediated genetic transformation is reported in this paper. Integration of transgene in the genome of transgenic plants was demonstrated using polymerase chain reaction and Southern-blot hybridization, while expression was ascertained by reverse transcription polymerase chain reaction. Histochemical analysis confirmed the expression of GUS enzyme in transformed shoots. Levels of endochitinase enzyme in transgenic plants were found to be up to 10 fold higher compared to control plants. Endochitinase enzyme of 42 kDa was also visualized on SDS-PAGE gel using fluorimetric zymogram in transgenic plants. Endochitinase activity was found to be higher in leaf and stem than the root tissue in transgenic tomato plants. Transgenic lines of both plants showed enhanced resistance to fungal pathogens and a strong negative correlation was found between expression level of endochitinase enzyme and size of disease lesions. Inheritance of transgene, expression and resistance to fungal pathogens of T 1 transgenic tobacco lines was also analysed. The results of the present studies show that ech42 is a promising candidate gene for developing fungal disease resistance in tomato plants.

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“…The effects of HpaG Xooc and its functional fragments on green tea provide an alternative to the organic planting system that integrates multiple measures in watering, fertilizing, and control of diseases and insects into a procedure of management applied throughout the farming season (Geldermann and Kogel 2002;Sauerborn 2002). Moreover, using proteins as the active ingredients of spraying mixtures (Fontanilla et al 2005a, b) is simpler, and more acceptable by the public in contrast to the recalcitrance regarding some other approaches, such as genetic engineering (Stuiver and Custers 2001;Jang et al 2006).…”

Section: Discussionmentioning

confidence: 97%

Productivity and biochemical properties of green tea in response to full-length and functional fragments of HpaGXooc, a harpin protein from the bacterial rice leaf streak pathogen Xanthomonas oryzae pv. oryzicola

Long

et al. 2007

J. Biosci.

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Harpin proteins from plant pathogenic bacteria can stimulate hypersensitive cell death (HCD), drought tolerance, defence responses against pathogens and insects in plants, as well as enhance plant growth. Recently, we identified nine functional fragments of HpaG;Xooc, a harpin protein from Xanthomonas oryzae pv.oryzicola, the pathogen that causes bacterial leaf streak in rice. Fragments HpaG;1-94'HpaG;10-42, and HpaG;62-138, which contain the HpaG;Xooc regions of the amino acid sequence as indicated by the number spans, exceed the parent protein in promoting growth, pathogen defence and HCD in plants. Here we report improved productivity and biochemical properties of green tea (Camellia sinensis) in response to the fragments tested in comparison with HpaG;Xooc and an inactive protein control. Field tests suggested that the four proteins markedly increased the growth and yield of green tea, and increased the leaf content of tea catechols, a group of compounds that have relevance in the prevention and treatment of human diseases. In particular, HpaG;1-94 was more active than HpaG;Xooc in expediting the growth of juvenile buds and leaves used as green tea material and increased the catechol content of processed teas. When tea shrubs were treated with HpaH;Xooc and HpaG;1-94 compared with a control, green tea yields were over 55% and 39% greater, and leaf catechols were increased by more than 64% and 72%, respectively. The expression of three homologues of the expansin genes, which regulate plant cell growth, and the CsCHS gene encoding a tea chalcone synthase, which critically regulates the biosynthesis of catechols, were induced in germinal leaves of tea plants following treatment with HpaG;1-94 or HpaG;Xooc. Higher levels of gene expression were induced by the application of HpaG;1-94 than HpaG;Xooc. Our results suggest that the harpin protein, especially the functional fragment HpaG;1-94, can be used to effectively increase the yield and improve the biochemical properties of green tea, a drink with medicinal properties.

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The hrpN gene of Erwinia amylovora stimulates tobacco growth and enhances resistance to Botrytis cinerea

Cited by 38 publications

References 26 publications

Fire Blight: Applied Genomic Insights of the Pathogen and Host

Fire Blight: Applied Genomic Insights of the Pathogen and Host

Expression of a fungal endochitinase gene in transgenic tomato and tobacco results in enhanced tolerance to fungal pathogens

Productivity and biochemical properties of green tea in response to full-length and functional fragments of HpaGXooc, a harpin protein from the bacterial rice leaf streak pathogen Xanthomonas oryzae pv. oryzicola

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