The HSS3/4 enhancer of Crlz1-IgJ locus is another target of EBF in the pre-B cell stage of B cell development

Kim, Ja‐Yeon; Park, Sung-Kyun; Kim, Hong Gi; Cho, Sun-Jung; Kim, Jiyoung; Kang, Chang-Joong

doi:10.1016/j.imlet.2006.07.007

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…In our hands, Crlz-1 was initially found to correspond to a novel pre-B cell-specific gene [ 4 , 5 ], which had been sought in the vicinity of IgJ gene locus on the mouse chromosome 5 because the same enhancer chromatin that was opened to express IgJ in the plasma cell stage [ 6 ] was also opened in the pre-B cell stage, despite the closed chromatin of IgJ gene locus and no IgJ expression, possibly to drive the pre-B cell-specific expression of a certain neighboring gene [ 7 ]. Notably, the promoter of Crlz-1 turned out to be bound by LEF-1 (lymphoid enhancer factor-1) with β-catenin in pre-B cells, which led to the discovery that this gene is a target of the canonical Wnt (wingless-related MMTV integration site) signaling pathway [ 8 , 9 ].…”

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confidence: 99%

Crlz-1 Homozygous Null Knockout Mouse Embryos Are Lethally Stopped in Their Early Development

Choi¹,

Pi²,

Jeong³

et al. 2022

Genes

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Although the conditional gene knockout (KO) is a better choice for observing its phenotype in a specific cell, tissue, and/or organ, the simple null gene KO could nevertheless be attempted initially to scan its overall phenotypes at the level of the whole-body system, especially for a new gene such as Crlz-1. Therefore, with a hope to glean phenotypic clues for Crlz-1 at the whole-body system, we attempted to generate its null KO mice. Contrary to our original desire, Crlz-1 homozygous null KO mice were not born. However, in the chasing of their homozygous KO embryos, they were found to be lethally impaired from early development, remaining in a state of small globular mass without ever leading to a body shape, indicating the critical role of Crlz-1 as a Wnt target gene for the proliferation and/or differentiation of cells during early mouse embryonic development.

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