The human choriocapillaris in organ culture

Vatne, Harald Overskott; Nicolaissen, Bjørn

doi:10.1111/j.1755-3768.1988.tb04051.x

Cited by 7 publications

(3 citation statements)

References 5 publications

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“…In previous studies, tissue cultures (Tso et al 1973;Overskott Vatne & Nicolaissen 1988;Fiskaadal et al 1992), choroidal cells from animals (Chader et al 1982; Morse et al 1989;Morse & Sidikaro 1990) or isolated subpopulations from the human choroid (Hu et al 1993) have been used to study the physiology of the choroid. Few attempts have been made to study the outgrowth of human choroidal cells in vitro ), characterize these cells and to investigate factors influencing their growth and behaviour.…”

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Isolation and in vitro propagation of human choroidal fibroblasts

Klyve

Nicolaissen

Næss

1996

Acta Ophthalmologica Scandinavica

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Subretinal ingrowth of choroidal fibroblasts is of importance in several clinical settings such as age-related macular degeneration or after traumas such as laser treatment and infection. In the present investigation we describe a method for isolation and propagation of human choroidal fibroblasts in vitro. Outgrowth from the outseeded choroid was studied by means of phase contrast microscopy, scanning electron microscopy and by immunohistochemical methods. The secondary cultures were found to contain almost only fibroblasts (more than 95% of the cells), some contaminating retinal pigment cells were also found. Choroidal fibroblasts contribute to the fibrovascular subretinal scarring process, and our cell culturing system seems to be a suitable tool for studying factors regulating the behavior of these cells in vitro.

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Isolation and in vitro propagation of human choroidal fibroblasts

Klyve

Nicolaissen

Næss

1996

Acta Ophthalmologica Scandinavica

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“…It has previously been shown that human choriocapillaris and choroidal cells seeded on Bruch's membrane can be studied in controlled tissue culture conditions (Overskott Vatne 1988;Nicolaissen et al 1988).…”

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In vitro ingrowth of choroidal cells on Bruch's membrane

Fiskaadal

Nicolaissen

Ringvold

et al. 1992

Acta Ophthalmologica

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Full thickness eyewall explants were made from human eyes, the retina and retinal pigment epithelium were removed, and the explants were maintained in vitro in Ham's F-10 medium with 20% foetal bovine serum. Cultured explants were examined by scanning and transmission electronmicroscopy after varying lengths of time. When in vitro, choroidal cells migrated across the cut edge of the explant onto the denuded Bruch's membrane where they subsequently showed production of extracellular matrix. In the human eye, ingrowth of choroidal cells on Bruch's membrane is found in macular degenerations, inflammatory chorioretinal disorders and subsequent to trauma. The in vitro model system presented in our study could facilitate evaluation of the dynamics underlying such ingrowth and also of factors affecting the course of this process.

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“…Incubation was for 24 hours at 37 o C in 5% CO 2 . The choriocapillaris has been shown to survive for up to one month in organ culture, so 24 hours was well within the anticipated survival curve for the tissue(135).To quantify the ICAM-1 protein expression of tissues from the C5a and control organ cultures, quantitative PCR was used. RNA was isolated from four C5a treated and four control organ culture punches from one human donor using the RNeasy mini kit according to manufacturer's instructions (Qiagen, Valencia, CA).…”

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Choroidal endothelial cell activation in age-related macular degeneration

Skeie¹

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Age-related macular degeneration (AMD) is a devastating ocular disease affecting one third of the elderly population in the western world. Some cases of AMD develop neovascular membranes, which are characterized by the pathologic growth of new blood vessels into the retina. This pathology may be initiated by proteins capable of activating endothelial cells to become angiogenic or inflammatory, later causing them to grow abnormally. This investigation aimed to determine the causes of pathologic blood vessel growth in AMD. Human eyes with AMD have been shown by us and others to have abnormal activities of angiogenin, complement component C5 anaphylatoxin (C5a), and/or elastin fragments. We therefore employed methods including PCR, immunoblotting, immunohistochemistry, morphometrics, tissue culture, ultrastructural observations, and functional assays to determine the effects angiogenin, C5a, and elastin fragments on the angiogenic and inflammatory changes of choroidal endothelial cells in vitro and in vivo. TABLE OF CONTENTS LIST OF TABLES……………………………………………………………………….vii LIST OF FIGURES……………………………………………………………………..viii LIST OF ABBREVIATIONS……………………………………………………………..x CHAPTER 1. BACKGROUND AND SIGNIFICANCE.

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The human choriocapillaris in organ culture

Cited by 7 publications

References 5 publications

Isolation and in vitro propagation of human choroidal fibroblasts

Isolation and in vitro propagation of human choroidal fibroblasts

In vitro ingrowth of choroidal cells on Bruch's membrane

Choroidal endothelial cell activation in age-related macular degeneration

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