The Human Gastrointestinal Secretory Immune System in Health and Disease

Brandtzæg, Per; Valnes, K; Scott, Helge; Rognum, Torleiv O.; Bjerke, K; Baklien, K

doi:10.3109/00365528509093765

Cited by 112 publications

(40 citation statements)

References 105 publications

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“…1). In keeping with previous studies (20) and with our immunocytological data obtained on enzymatically dispersed duodenal cell suspensions, the majority (mean 72%) of the ISC in the duodenum were accounted for by IgA ISC. IgG and IgM ISC were also detected, but in considerably lower frequencies.…”

Section: Resultssupporting

confidence: 66%

“…This is in keeping with the many studies, and our own immunocytological data, showing that IgA is the dominating immunoglobulin as well as specific antibody isotype present in human mucosal glandular tissues and external secretions (20,21). On the other hand, our findings are at variance with those recently reported by Peters et al (22) who, using an AP-based ELISPOT technique, reported similar numbers of cells secreting IgA, IgG, and IgM among small intestinal MNC.…”

Section: Discussioncontrasting

confidence: 57%

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Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-gamma production and evokes local immunological memory.

Quiding

Nordström²,

Kilander³

et al. 1991

J. Clin. Invest.

211

123

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We have examined secretory antibody and cell-mediated immune responses to oral cholera vaccine in the human gastrointestinal mucosa. Freshly isolated peripheral blood lymphocytes and intestinal lymphocytes obtained by enzymatic dispersion of duodenal biopsies were assayed for numbers of total and vaccine specific immunoglobulin-secreting cells by enzyme-linked immunospot assay (ELISPOT) techniques; the frequency of cells secreting interferon-y (IFN--y) was also examined by a new modification of the ELISPOT technique.After booster immunizations with oral cholera vaccine, large numbers of cholera toxin-specific antibody-secreting cells (ASC) appeared in the small intestine. The responses were dominated by IgA ASC. A single immunization, performed 5 mo after the initial vaccinations, gave rise to an ASC response similar to that seen after the first booster immunization, with respect to both magnitude and isotype distribution. Each of the immunizations also evoked an ASC response in blood which was of lower magnitude than that seen in the small intestine, and comprised similar proportions of IgA and IgG ASC. A booster immunization also resulted in increased frequencies of IFN-y-secreting cells, but this increase was confined to the duodenal mucosa.This study establishes the feasibility of studying, at the single-cell level, intestinal immune reactivity in humans. Furthermore, it indicates that the small intestinal mucosa is an enriched source of IFN-7. It also demonstrates marked differences between intestinal and peripheral blood immune responses after enteric immunization, and confirms the notion that the mucosal immune system in humans displays immunological memory. (J. Clin. Invest. 1991. 88:143-148.)

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Section: Resultssupporting

confidence: 66%

Section: Discussioncontrasting

confidence: 57%

Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-gamma production and evokes local immunological memory.

Quiding

Nordström²,

Kilander³

et al. 1991

J. Clin. Invest.

211

123

View full text Add to dashboard Cite

show abstract

“…The use of a control group with UC helped to avoid other bias such as diet modification. In thie patients witti CD, jejunat secretion of ptgA, ttie tnain component of ttie tiumoral imtnune mucosal defence of ttie jejunum (Brandtzaeg et al, 1985), was tialf of ttie normal figure. Secretion rate of orosomucoid was increased; tiowever, its RCE remained in ttie normal range, demonstrating ttiat ttie tiigti secretion rate was only ttie consequence of ttie increased plasma concentration of orosomucoid, wtiicti is secreted via a motecutar weigtit-affected seepage from ptasma (Jonard et ai, 1984).…”

Section: Discussionmentioning

confidence: 99%

Immunological study of histologically non-involved jejunum during Crohn's disease: evidence for reduced in vivo secretion of secretory IgA

Marteau

Colombel

Németh

et al. 1990

Clinical and Experimental Immunology

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SUMMARYWe studied the local humoral immunity of histologically non-involved jejunum in patients with Crohn's disease. Normal subjects and patients with uleerative colitis served as eontrols. Jejunal fiuid samples were collected during segmental jejunal perfusion, under an occluding balloon and the in vivo jejunal secretion of the following proteins was determined: albumin, orosomucoid, transferrin, a2-macroglobulin, secretory component, monomeric and polymeric IgA, IgG, and IgM. The densities and number of IgA-, IgG-, and IgM-containing cells in the lamina propria of the jejunum were measured on perendoscopic biopsies. Jejunal secretion of polymeric IgA and the density of IgAcontaining plasma cells in the lamina propria were significantly lower in patients with Crohn's disease than in both control groups. This abnormal intestinal immune response, which was not correlated to the activity of the disease, might be involved in its pathogenesis.

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“…Some of the LP lymphocytes are PP-derived B lymphoblasts, becoming mature plasma cells in the LP. They preferentially produce IgA (5,6). The majority of T cells in the LP are CD4+ (7,8).…”

mentioning

confidence: 99%

The Postnatal Development of Gut Lamina Propria Lymphocytes: Number, Proliferation, and T and B Cell Subsets in Conventional and Germ-Free Pigs

1991

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The gut immune system is an essential part of the bamer function of the gut wall. The uptake of microbial and nutritional antigens in the gut starts immune responses by initiating either tolerance or a specific secretory immune reaction (for review see refs. 1-4). Large numbers of lymphocytes are distributed in the epithelium and LP of the gut mucosa. Some of the LP lymphocytes are PP-derived B lymphoblasts, becoming mature plasma cells in the LP. They preferentially produce IgA (5, 6). The majority of T cells in the LP are CD4+ (7,8). The T cells show a high expression of genes associated with cell activation (9). The proportion of antigen-primed memory cells is higher in LP T cells than in peripheral blood lymphocytes (reviewed in 4).In the early postnatal period, the gut wall is exposed to microbial antigens for the first time. Additionally, the amount of

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The Human Gastrointestinal Secretory Immune System in Health and Disease

Cited by 112 publications

References 105 publications

Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-gamma production and evokes local immunological memory.

Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-gamma production and evokes local immunological memory.

Immunological study of histologically non-involved jejunum during Crohn's disease: evidence for reduced in vivo secretion of secretory IgA

The Postnatal Development of Gut Lamina Propria Lymphocytes: Number, Proliferation, and T and B Cell Subsets in Conventional and Germ-Free Pigs

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