The human T cell antigen receptor repertoire: skewed use of Vβ gene families by CD8+ cells

Clarke, Gillian R.; Humphrey, C. A.; Lancaster, F.; Boylston, Arthur W.

doi:10.1111/j.1365-2249.1994.tb06568.x

Cited by 31 publications

(1 citation statement)

References 25 publications

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“…Vβ5. 1, 6.7, 8, 9 and 12), others in CD8+ cells, such as Vβ1, Vβ5.2, Vβ9, Vβ14 and Vβ23 (36)(37)(38)(39)(40)(41). In our control group, Vβ3, 14, 16, 20 and 21.3 dominated the repertoire for both T subsets and together, as these chains answered for about 43 and 40% of the circulating T CD4+ and CD8+ cells, respectively.…”

Section: Discussionmentioning

confidence: 66%

Flow Cytometry Evaluation of the T-Cell ReceptorVβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy

et al. 2005

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HIV-1 infection leads to serious impairment of the immune system and perturbations in the T cell receptor Vbeta repertoire are also described. Immune reconstitution can be potentially achieved in response to HAART. In the present study 10 patients were investigated for the Vbeta pattern expression before and after six months of HAART. TCR were analyzed for T CD4+ and CD8+ subsets, separately, by flow cytometry, using a monoclonal antibody set of 24 different Vbeta chains. Compared to eight Brazilian healthy controls, no differences in Vbeta pattern of expression was observed for patients before or on antiretroviral therapy. Some chains such as Vbeta 3, 14, 16, 20 and 21.3 were over utilized by both T subsets, independently of HIV infection and/or antiretroviral treatment, differing from the ones described for individuals of other nationalities. However, when each patient was taken individually, particular alterations were detected for the Vbeta gene usage, compared to controls, for all individuals. After treatment, significant Vbeta usage changes were observed for seven patients. One or more chains on both T subsets were engaged in this process, defining a preferential oligoclonal profile for TCR repertoire distribution, after HAART. Although no pattern of specific Vbeta changes was detected in the circulating T cells, we cannot exclude that differential immune responses to HIV or other important antigens are being focused by these cells.

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Section: Discussionmentioning

confidence: 66%

Flow Cytometry Evaluation of the T-Cell ReceptorVβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy

et al. 2005

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T cell repertoire in patients with multiple myeloma and monoclonal gammopathy of undetermined significance: Clonal CD8⁺ T cell expansions are found preferentially in patients with a low tumor burden

Halapi

Werner²,

Wahlström

et al. 1997

Eur J Immunol

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The T cell receptor (TCR) variable (V) gene repertoire was analyzed in patients with monoclonal gammopathy of undetermined significance (MGUS) (n = 17), multiple myeloma (MM) stage I (n = 16), MM stages II/III (n = 31) and age-matched controls (n = 27) by immunofluorescence and flow cytometry using a panel of mouse monoclonal antibodies (mAb) (n = 10) against TCR V alpha and V beta gene products. T cell expansion was defined as a value > or = thrice the normal median value for each respective TCR V mAb. Fifty-three percent of all patients displayed CD8+ expansion(s) as compared to 30% of age-matched controls (p < 0.001). Within the CD4 subset, 18% of the patients displayed T cell expansion(s) in comparison to 11% of the controls (not significant). Interestingly, the CD8+ expansion(s) were more frequently noted in patients with a low tumor burden (MGUS/MMI) (73%) as compared to those with advanced disease (MM II/III) (32% and control donors (30%) (p < 0.01). Likewise, multiple CD8+ expansions (two or more) were more common in MGUS/MM I patients than in MM II/III and controls (p < 0.01). The T cell expansions were stable over time in patients with a stable disease. A high degree of clonality of the expansions was detected by TCR CDR3 fragment length analysis, determination of J beta gene usage and nucleotide sequencing. The frequent finding of oligoclonal CD8+ T cell expansions in patients with a low tumor mass, but not in patients with advanced disease justifies further work in order to identify the relevance of expanded CD8+ T cells. In one patient with T cell reactivity against the autologous myeloma idiotype, two expansions within the CD8 population (V beta 3 and V beta 5.2 respectively) displayed no reactivity against the idiotype. Instead, idiotype recognition was confined to a CD8 non-expanded V beta 22+ T cell population, with a highly restricted TCR usage (CDR3 fragment length analysis).

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Idiosyncratic Alterations of TCR Size Distributions Affecting Both CD4 and CD8 T Cell Subsets in Aging Mice

Mosley

Koker

Miller

1998

Cellular Immunology

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The human T cell antigen receptor repertoire: skewed use of Vβ gene families by CD8+ cells

Cited by 31 publications

References 25 publications

Flow Cytometry Evaluation of the T-Cell ReceptorVβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy

Flow Cytometry Evaluation of the T-Cell ReceptorVβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy

T cell repertoire in patients with multiple myeloma and monoclonal gammopathy of undetermined significance: Clonal CD8⁺ T cell expansions are found preferentially in patients with a low tumor burden

Idiosyncratic Alterations of TCR Size Distributions Affecting Both CD4 and CD8 T Cell Subsets in Aging Mice

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The human T cell antigen receptor repertoire: skewed use of Vβ gene families by CD8+ cells

Cited by 31 publications

References 25 publications

Flow Cytometry Evaluation of the T-Cell ReceptorVβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy

Flow Cytometry Evaluation of the T-Cell ReceptorVβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy

T cell repertoire in patients with multiple myeloma and monoclonal gammopathy of undetermined significance: Clonal CD8+ T cell expansions are found preferentially in patients with a low tumor burden

Idiosyncratic Alterations of TCR Size Distributions Affecting Both CD4 and CD8 T Cell Subsets in Aging Mice

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T cell repertoire in patients with multiple myeloma and monoclonal gammopathy of undetermined significance: Clonal CD8⁺ T cell expansions are found preferentially in patients with a low tumor burden