We study Σ 1 (ω 1 )-definable sets (i.e. sets that are equal to the collection of all sets satisfying a certain Σ 1 -formula with parameter ω 1 ) in the presence of large cardinals. Our results show that the existence of a Woodin cardinal and a measurable cardinal above it imply that no well-ordering of the reals is Σ 1 (ω 1 )-definable, the set of all stationary subsets of ω 1 is not Σ 1 (ω 1 )definable and the complement of every Σ 1 (ω 1 )-definable Bernstein subset of ω 1 ω 1 is not Σ 1 (ω 1 )-definable. In contrast, we show that the existence of a Woodin cardinal is compatible with the existence of a Σ 1 (ω 1 )-definable wellordering of H(ω 2 ) and the existence of a ∆ 1 (ω 1 )-definable Bernstein subset of ω 1 ω 1 . We also show that, if there are infinitely many Woodin cardinals and a measurable cardinal above them, then there is no Σ 1 (ω 1 )-definable uniformization of the club filter on ω 1 . Moreover, we prove a perfect set theorem for Σ 1 (ω 1 )-definable subsets of ω 1 ω 1 , assuming that there is a measurable cardinal and the non-stationary ideal on ω 1 is saturated. The proofs of these results use iterated generic ultrapowers and Woodin's Pmax-forcing. Finally, we also prove variants of some of these results for Σ 1 (κ)-definable subsets of κ κ, in the case where κ itself has certain large cardinal properties.2010 Mathematics Subject Classification. 03E45, 03E47, 03E55.