The in vitro elution of gentamicin sulfate from a commercially available gentamicin‐loaded acrylic bone cement, VersaBond™ AB

Abstract

The present study was designed to yield results that would be used to contribute to the ongoing debate about the mechanism of the in vitro elution of an antibiotic from an antibiotic-loaded acrylic bone cement. To this end, the elution rates (R) of gentamicin sulfate (expressed as a weight percentage of the initial mass of the antibiotic in the specimen, normalized with respect to the duration of the test) from statically loaded (STATIC) and dynamically loaded (+/-10 MPa; 2 Hz; until fracture; DYNAMIC) specimens fabricated from a commercially available acrylic bone cement (VersaBond AB), in phosphate-buffered saline solution at 37 degrees C, were obtained with the use of a spectrophotometric method. There was evidence of microcracking in the fracture surfaces of DYNAMIC specimens, but no such evidence in the case of STATIC specimens. The surface area of the DYNAMIC specimens, during the tensile phase of the cyclical loading, was estimated to be about 3% larger than for the STATIC specimens (1742 mm(2) versus 1696 mm(2)). The bulk porosities P of the specimens in both sets were also determined and found to not be statistically different, with P for the STATIC and DYNAMIC specimens being 8.55 +/- 0.10 and 8.88 +/- 0.18%, respectively. At the end of the test period, R was found to be 0.36 +/- 0.20 and 1.28 +/- 0.14 wt %/day for the STATIC and DYNAMIC specimens, respectively. It is suggested that the present results provide support for the postulate that the elution mechanism of gentamicin in this cement is a surface phenomenon.

Nanostructured material formulated acrylic bone cements with enhanced drug release

“…This result is consistent with reported investigation of commercially available GTMC-loaded acrylic bone cement and it was postulate that the elution mechanism of GTMC in this type of cement is a surface phenomenon [30].…”

Preparation and properties of electrospun PAN/Fe3O4 magnetic nanofibers

“…All the specimens were made by the same person (Cai). With the procedure described by Lewis and Janna,16 the specimens were screened, polished, cleaned, and sterilized before elution test.…”

Dependence of in vitro fatigue properties of PMMA bone cement on the polydispersity index of its powder

“…For each specimen, three discs and four randomly selected discrete fields on each disc were examined. Further details of this porosity determination method are given in a previous report (Lewis and Janna, 2004). …”