TheKLF6Super Enhancer Modulates Cell ProliferationviaMiR-1301 in Human Hepatoma Cells

Abstract

Background: Recent studies have attempted to elucidate the function of super enhancers by means of microRNAs. Although the functional outcomes of miR-1301 have become clearer, the pathways that regulate the expressions of miR-1301 remain unclear. Objective: The objective of this paper was to consider the pathway regulating expression of miR- 1301 and miR-1301 signaling pathways with the inhibition of cell proliferation. Methods: In this study, we prepared the cell clones that the KLF6 super enhancer was deleted by means of the CRISPR/Cas9 system-mediated genetic engineering. Changes in miR-1301 expression after the deletion of the KLF6 super enhancer were evaluated by RT-PCR analysis, and the signal pathway of miR-1301 with inhibition of the cell proliferation was examined using RNA interference technology. Results: The results showed that miR-1301 expression was significantly increased after the deletion of the KLF6 super enhancer. Over-expression of miR-1301 induced by deletion of the KLF6 super enhancer also regulated the expression of p21 and p53 in human hepatoma cells. functional modeling of findings using siRNA specific to miR-1301 showed that expression level changes had direct biological effects on cellular proliferation in Human hepatoma cells. Furthermore, cellular proliferation assay was shown to be directly associated with miR-1301 levels. Conclusion: As a result, it was demonstrated that the over-expression of miR-1301 induced by the disruption of the KLF6 super enhancer leads to a significant inhibition of proliferation in HepG2 cells. Moreover, it was demonstrated that the KLF6 super enhancer regulates the cell-proliferative effects which are mediated, at least in part, by the induction of p21and p53 in a p53-dependent manner. Our results provide the functional significance of miR-1301 in understanding the transcriptional regulation mechanism of the KLF6 super enhancer.

Super-enhancers: a new frontier for epigenetic modifiers in cancer chemoresistance

“…found that heparinase eRNA enhances chromatin looping between the SE and promoter in several cancer cell lines, promoting tumorigenesis in vitro and in vivo [ 103 ]. Klf6 is a gene responsible for tumorigenesis, and the loss of the Klf6 SE was found to inhibit the proliferation of liver cancer cells by upregulating miR-1301 [ 104 ]. lncRNA HCCL5 is an SE-driven gene that confers the malignant phenotypes of liver cancer cells [ 105 ].…”

The emerging role of super enhancer-derived noncoding RNAs in human cancer

“…In recent years, SE-derived ncRNAs have been found to play an important role in promoting the malignant proliferation of tumors ( Figure 3 ). Some SE-derived ncRNAs affect proliferation-related signaling pathways, thus leading to the unlimited proliferation of tumor cells 62 . Xie et al 63 reported a squamous cell carcinoma (SCC)-specific SE-derived lncRNA LINC01503.…”

mir‐22‐3p/KLF6/MMP14 axis in fibro‐adipogenic progenitors regulates fatty infiltration in muscle degeneration

“…miRNAs are critical in preadipocyte adipogenesis, and KLF6 is also regulated by several miRNAs 13,[21][22][23][24] ; thus, we investigated whether the expression of KLF6 in FAPs was regulated by miRNAs. We analyzed several miRNAs that were reported to regulate adipogenesis.…”