Background: Recent studies have attempted to elucidate the function of super enhancers by means of microRNAs. Although the functional outcomes of miR-1301 have become clearer, the pathways that regulate the expressions of miR-1301 remain unclear. Objective: The objective of this paper was to consider the pathway regulating expression of miR- 1301 and miR-1301 signaling pathways with the inhibition of cell proliferation. Methods: In this study, we prepared the cell clones that the KLF6 super enhancer was deleted by means of the CRISPR/Cas9 system-mediated genetic engineering. Changes in miR-1301 expression after the deletion of the KLF6 super enhancer were evaluated by RT-PCR analysis, and the signal pathway of miR-1301 with inhibition of the cell proliferation was examined using RNA interference technology. Results: The results showed that miR-1301 expression was significantly increased after the deletion of the KLF6 super enhancer. Over-expression of miR-1301 induced by deletion of the KLF6 super enhancer also regulated the expression of p21 and p53 in human hepatoma cells. functional modeling of findings using siRNA specific to miR-1301 showed that expression level changes had direct biological effects on cellular proliferation in Human hepatoma cells. Furthermore, cellular proliferation assay was shown to be directly associated with miR-1301 levels. Conclusion: As a result, it was demonstrated that the over-expression of miR-1301 induced by the disruption of the KLF6 super enhancer leads to a significant inhibition of proliferation in HepG2 cells. Moreover, it was demonstrated that the KLF6 super enhancer regulates the cell-proliferative effects which are mediated, at least in part, by the induction of p21and p53 in a p53-dependent manner. Our results provide the functional significance of miR-1301 in understanding the transcriptional regulation mechanism of the KLF6 super enhancer.

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In this paper, the Haar wavelet discretization method (HWDM) is proposed for the calculation of natural frequency of the laminated composite conical-cylindrical coupled shells. The displacement components of the system are set by the first order shear deformation shell theory (FSDST), and all the displacement functions of conical and cylindrical shells including boundary conditions are obtained by the Haar wavelet and its integrals. The Hamilton’s principle is applied to the constitution equation and the artificial spring technique is introduced to generalize the boundary and continuity conditions of the coupled structure. The convergence and accuracy of the proposed method are validated by comparing with the results obtained from the previous works and finite element analysis, and the proposed method shows high accuracy, reliability and good convergence in both individual structure and coupled structure. Using the proposed method, in the laminated composite conical–cylindrical coupled shell with any boundary condition, new numerical results on the natural frequency are obtained along with the parameter studies, and these results can provide the referential data for the following research in this field.

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