2005
DOI: 10.1104/pp.105.059550
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TheLPB1Gene Is Important for Acclimation ofChlamydomonas reinhardtiito Phosphorus and Sulfur Deprivation

Abstract: Organisms exhibit a diverse set of responses when exposed to low-phosphate conditions. Some of these responses are specific for phosphorus limitation, including responses that enable cells to efficiently scavenge phosphate from internal and external stores via the production of high-affinity phosphate transporters and the synthesis of intracellular and extracellular phosphatases. Other responses are general and occur under a number of different environmental stresses, helping coordinate cellular metabolism and… Show more

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Cited by 64 publications
(41 citation statements)
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“…Glutaraldehyde-treated or heat-treated cells were used as negative controls and showed no signal after CMFDA staining. The viability data we obtained with CMFDA staining was consistent with what was described previously using a different method (Chang et al, 2005).…”
Section: Cell Viability Analysissupporting
confidence: 88%
“…Glutaraldehyde-treated or heat-treated cells were used as negative controls and showed no signal after CMFDA staining. The viability data we obtained with CMFDA staining was consistent with what was described previously using a different method (Chang et al, 2005).…”
Section: Cell Viability Analysissupporting
confidence: 88%
“…Quantitative real-time PCR (qRT-PCR) on CrLIP1 was performed using the primers qRT-F and qRT-R on the Applied Biosystems 7500 Fast real-time PCR (RT-PCR) system. The data were normalized to the commonly used RACK1 gene using the primers RACK1-F (receptor for activated C kinase 1 forward) and RACK1 reverse (RACK1-R) (9). The 2 Ϫ⌬⌬CT method (28) was employed for data analysis.…”
Section: Methodsmentioning
confidence: 99%
“…at 258C, on a 12 h L : 12 h D cycle. Phosphorus-and sulphur-deprived cultures were grown in TAP lacking phosphorus and sulphur, respectively (Chang et al 2005); light-deprived cultures were grown in regular TAP, but flasks were covered in aluminium foil. To inhibit the photosynthetic electron flow, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU; 50 mM stock in 95% ethanol) was added to a final concentration of 10 mM (Wykoff et al 1998).…”
Section: Methodsmentioning
confidence: 99%