The <i>R1</i> gene for potato resistance to late blight (<i>Phytophthora infestans</i>) belongs to the leucine zipper/NBS/LRR class of plant resistance genes

Ballvora, Agim; Ercolano, Maria Raffaella; Weiß, Julia; Meksem, Khalid; Bormann, Christina Angelika; Oberhagemann, Petra; Salamini, F.; Gebhardt, Christiane

doi:10.1046/j.1365-313x.2001.01292.x

Cited by 367 publications

(283 citation statements)

References 55 publications

Supporting

Mentioning

277

Contrasting

Unclassified

Order By: Relevance

“…To date, four late blight R-genes have been cloned: R1 (Ballvora et al 2002), R3a (Huang et al 2005), RB or Rpiblb1 (Song et al 2003;van der Vossen et al 2003) and Rpiblb2 (van der Vossen et al 2005). The latter two genes were cloned from S. bulbocastanum and confer resistance to all P. infestans isolates tested so far.…”

Section: Introductionmentioning

confidence: 99%

Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum

et al. 2008

View full text Add to dashboard Cite

Allele mining facilitates the discovery of novel resistance (R) genes that can be used in breeding programs and sheds light on the evolution of R genes. Here we focus on two R genes, Rpi-blb1 and Rpi-blb2, originally derived from Solanum bulbocastanum. The Rpi-blb1 gene is part of a cluster of four paralogues and is Xanked by RGA1-blb and RGA3-blb. Highly conserved RGA1-blb homologues were discovered in all the tested tuber-bearing (TB) and non-tuber-bearing (NTB) Solanum species, suggesting RGA1-blb was present before the divergence of TB and NTB Solanum species. The frequency of the RGA3-blb gene was much lower. Interestingly, highly conserved Rpi-blb1 homologues were discovered not only in S. bulbocastanum but also in Solanum stoloniferum that is part of the series Longipedicellata. Resistance assays and genetic analyses in several F1 populations derived from the relevant late blight resistant parental genotypes harbouring the conserved Rpi-blb1 homologues, indicated the presence of four dominant R genes, designated as Rpi-sto1, Rpi-plt1, Rpi-pta1 and Rpi-pta2. Furthermore, Rpi-sto1 and Rpi-plt1 resided at the same position on chromosome VIII as Rpi-blb1 in S. bulbocastanum. Segregation data also indicated that an additional unknown late blight resistance gene was present in three populations. In contrast to Rpi-blb1, no homologues of Rpi-blb2 were detected in any material examined. Hypotheses are proposed to explain the presence of conserved Rpi-blb1 homologues in S. stoloniferum. The discovery of conserved homologues of Rpi-blb1 in EBN 2 tetraploid species oVers the possibility to more easily transfer the late blight resistance genes to potato varieties by classical breeding.

show abstract

Section: Introductionmentioning

confidence: 99%

Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Soon afterwards, some of them have been introduced into potato cultivars (Niederhauser et al 1954;Stewart and Bradshaw 2001). The Wrst gene for late blight resistance that was mapped and subsequently cloned in potato is R1 on chromosome V ( Leonards-Schippers et al 1994;Ballvora et al 2002). Other R genes originating from S. demissum were mapped to diVerent positions.…”

Section: Introductionmentioning

confidence: 99%

The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period

et al. 2006

View full text Add to dashboard Cite

Despite the long history of breeding potatoes resistant to Phytophthora infestans, this oomycete is still economically the most important pathogen of potato worldwide. The correlation of high levels of resistance to late blight with a long vegetation period is one of the bottlenecks for progress in breeding resistant cultivars of various maturity types. Solanum phureja was identiWed as a source of eVective late blight resistance, which was transferred to the cultivated gene pool by interspeciWc crosses with dihaploids of Solanum tuberosum. A novel major resistance locus, Rpiphu1, derived most likely from S. phureja and conferring broad-spectrum resistance to late blight, was mapped to potato chromosome IX, 6.4 cM proximal to the marker GP94. Rpi-phu1 was highly eVective in detached leaXet, tuber slice and whole tuber tests during 5 years of quantitative phenotypic assessment. The resistance did not show signiWcant correlation with vegetation period length. Our Wndings provide a well-characterized new source of resistance for breeding early and resistant-to-P. infestans potatoes.

show abstract

“…In addition to Rpi-blb1/RB and Rpi-blb2, the S. demissum derived R1 (Ballvora et al 2002) and R3a (Huang 2005) have also been isolated using the map-based cloning approach. The first essential step for map-based gene cloning is to determine the accurate position of the target gene.…”

Section: Introductionmentioning

confidence: 99%

High-resolution Mapping and Analysis of the Resistance Locus Rpi-abpt Against Phytophthora infestans in Potato

et al. 2005

View full text Add to dashboard Cite

Introduction of more durable resistance against Phytophthora infestans causing late blight into the cultivated potato is of importance for sustainable agriculture. We identified a new monogenically inherited resistance locus that is localized on chromosome 4. The resistance is derived from an ABPT clone, which is originally a complex quadruple hybrid in which Solanum acaule, S. bulbocastanum, S. phureja and S. tuberosum were involved. Resistance data of the original resistant accessions of the wild species and analysis of mobility of AFLP markers linked to the resistance locus suggest that the resistance locus is originating from S. bulbocastanum. A population of 1383 genotypes was screened with two AFLP markers flanking the Rpi-abpt locus and 98 recombinants were identified. An accurate high-resolution map was constructed and the Rpi-abpt locus was localized in a 0.5 cM interval. One AFLP marker was found to cosegregate with the Rpi-abpt locus. Its DNA sequence was highly similar with sequences found on a tomato BAC containing several resistance gene analogues on chromosome 4 and its translated protein sequence appeared to be homologous to several disease resistance related proteins. The results indicated that the Rpi-abpt gene is a member of an R gene cluster.

show abstract

The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes

Cited by 367 publications

References 55 publications

Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum

Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum

The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period

High-resolution Mapping and Analysis of the Resistance Locus Rpi-abpt Against Phytophthora infestans in Potato

Contact Info

Product

Resources

About