The<i>Shigella flexneri</i>serotype Y vaccine candidate SFL124 originated from a serotype 2a background

Roberts, Fleur; Jennison, Amy V.; Verma, Naresh K.

doi:10.1016/j.femsim.2005.05.002

Cited by 9 publications

(11 citation statements)

References 22 publications

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“…Serotype Y strains carrying a defective gtrII gene have been reported previously. One case was an insertion sequence disruption of gtrII, while another was a single amino acid residual change leading to an inactive GtrII (5,14). The higher number of gtrII mutations found in serotype 2a and 2b S. flexneri strains may be due to a higher proportion of these serotypes in nature.…”

Section: Vol 49 2011 New Multiplex Pcr Assay For Serotyping S Flexmentioning

confidence: 99%

Development of a Multiplex PCR Assay Targeting O-Antigen Modification Genes for Molecular Serotyping of Shigella flexneri

et al. 2011

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Shigella flexneri is the major Shigella species that causes diarrheal disease in developing countries. It is further subdivided into 15 serotypes based on O-antigen structure. Serotyping of S. flexneri is important for epidemiological purposes. In this study, we developed a multiplex PCR assay targeting the O-antigen synthesis gene wzx and the O-antigen modification genes gtrI, gtrIC, gtrII, oac, gtrIV, gtrV, and gtrX for molecular serotyping of S. flexneri. The multiplex PCR assay contained eight sets of specific PCRs in a single tube and can identify 14 of the 15 serotypes (the exception being serotype Xv) of S. flexneri recognized thus far. A nearly perfect concordance (97.8%) between multiplex PCR assay and slide agglutination was observed when 358 S. flexneri strains of various serotypes were analyzed, except that 8 strains were carrying additional cryptic and/or defective serotype-specific genes. The multiplex PCR assay provides a rapid and specific method for the serotype identification of S. flexneri.

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Section: Vol 49 2011 New Multiplex Pcr Assay For Serotyping S Flexmentioning

confidence: 99%

Development of a Multiplex PCR Assay Targeting O-Antigen Modification Genes for Molecular Serotyping of Shigella flexneri

et al. 2011

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“…Roborts et al [27] found that the serotype Y vaccine candidate SFL124 contained an insertion sequence (IS1) in the gtrII gene, resulting in a nonfunctional GtrII. Chen et al [28] also observed the conversion of serotype 2a to serotype Y due to a single base mutation in gtrII which led to a Cys (437) to Tyr substitution.…”

Section: Resultsmentioning

confidence: 99%

Identification and Characterization of a Novel Shigella flexneri Serotype Yv in China

et al. 2013

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Shigella flexneri is the major cause of bacterial shigellosis in developing countries. S. flexneri is divided into at least 19 serotypes, the majority of which are modifications of the same basic O-antigen by glucosylation and/or O-acetylation of its sugar residues by phage encoded serotype-converting genes. Recently, a plasmid encoded phosphoethanolamine (PEtN) modification of the O-antigen has been reported, which is responsible for the presence of the MASF IV-1 determinant and results in conversion of traditional serotypes X, 4a and Y to novel serotypes Xv, 4av and Yv, respectively. In this study, we characterized 19 serotype Yv strains isolated in China. A variant of the O-antigen phosphoethanolamine transferase gene opt (formerly called lpt-O) carried by a pSFxv_2-like plasmid was found in serotype Yv strains, which specifies the phosphorylation pattern on the O-antigen of this serotype. For the majority of the O-antigen units, the PEtN modification occurs on RhaIII, while for a minority, modifications occur on both RhaII and RhaIII. Serotype-specific gene detection and PFGE analysis suggested that these serotype Yv isolates were originated from serotypes Y, Xv and 2a by acquisition of an opt-carrying plasmid and/or inactivation of serotype-specific gene gtrII or gtrX. These data, combined with those of serotypes Xv and 4av reported earlier, demonstrate that the plasmid-encoded PEtN modification is an important serotype conversion mechanism in S. flexneri, in addition to glucosylation and O-acetylation.

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“…In addition, the related 1 J C-1,1-H heteronuclear coupling constant of 169.8 Hz also proved the α-linkage. [68] (9). First, coupling of the latter with 5-[(benzyloxycarbonyl)amino]pentanol by means of N-iodosuccinimide (NIS) in the presence of a catalytic amount of trifluoromethanesulfonic acid (TfOH) in dichloromethane afforded the rhamnoside 10 in 80 % yield, which was then debenzoylated with sodium methoxide in methanol to provide the rhamnosyl acceptor 11 in a virtually quantitative yield.…”

Section: Resultsmentioning

confidence: 99%

“…The victims are mostly young children of the developing world but often also humans in industrialized countries. [1][2][3][4][5][6][7][8][9] Shigella flexneri, [10][11][12][13] a gramnegative enteropathogenic bacterium, is responsible for the endemic form of shigellosis, a highly infectious dysenteric syndrome in humans that causes diarrheal fever, violent cramps, and discharge of mucous membranes and bloody stools. [14][15][16][17][18][19] Shigellosis is characterized by a high morbidity and mortality.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Pentasaccharide Fragments Related to the O‐Specific Polysaccharide of Shigella flexneri Serotype 1a

Lemanski

Ziegler

2006

Eur J Org Chem

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The synthesis of the pentasaccharide 5‐aminopentylglycosides α‐L‐Rhap‐(1→3)‐[α‐D‐Glcp‐(1→4)]‐β‐D‐GlcpNAc‐(1→2)‐α‐L‐Rhap‐(1→2)‐α‐L‐Rhap‐1‐O‐(CH2)5NH2 (29) andα‐L‐Rhap‐(1→3)‐α‐L‐Rhap‐(1→3)‐[α‐D‐Glcp‐(1→4)]‐β‐D‐GlcpNAc‐(1→2)‐α‐L‐Rhap‐1‐O‐(CH2)5NH2 (28), related to the O‐specific polysaccharide of Shigella flexneri serotype 1a by coupling of the suitably protected trisaccharides α‐D‐Glcp‐(1→4)‐β‐D‐Glcp(1→2)‐α‐L‐Rhap‐1‐O‐(CH2)5NH2 (23) and α‐L‐Rhap‐(1→3)‐[α‐D‐Glcp‐(1→4)]‐β‐D‐Glcp‐1‐SPh (26) with the corresponding rhamnosyl glycosides α‐L‐Rhap‐(1→3)‐α‐L‐Rhap‐1‐SEt (17) and α‐L‐Rhap‐(1→3)‐α‐L‐Rhap‐1‐O‐(CH2)5NH2 (13), is described. Building blocks 23 and 26 were prepared by intramolecular glycosylation of an unsymmetrically tethered cellobiosamine derivative. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)

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TheShigella flexneriserotype Y vaccine candidate SFL124 originated from a serotype 2a background

Cited by 9 publications

References 22 publications

Development of a Multiplex PCR Assay Targeting O-Antigen Modification Genes for Molecular Serotyping of Shigella flexneri

Development of a Multiplex PCR Assay Targeting O-Antigen Modification Genes for Molecular Serotyping of Shigella flexneri

Identification and Characterization of a Novel Shigella flexneri Serotype Yv in China

Synthesis of Pentasaccharide Fragments Related to the O‐Specific Polysaccharide of Shigella flexneri Serotype 1a

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