The ICESt1 element of Streptococcus thermophilus belongs to a large family of integrative and conjugative elements that exchange modules and change their specificity of integration

Burrus, Vincent; Pavlovic, Guillaume; Decaris, Bernard; Guédon, Gérard

doi:10.1016/s0147-619x(02)00102-6

Cited by 130 publications

(181 citation statements)

References 62 publications

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“…All 18 genes cluster around rapI and phrI and are in the 20-kb ICEBs1 element (Fig. 2) previously identified by comparative sequence analysis (16). ICEBs1 is flanked by 60-bp direct repeats, the likely att sites.…”

Section: Identification Of a Mobile Genetic Element Regulated By Peptidementioning

confidence: 83%

“…Donors and recipients were grown in LB (for matings with Bacillus) or brain heart infusion (BHI) medium (for matings with Listeria) when assaying transfer from cells overexpressing rapI or in defined minimal medium when assaying transfer from cells treated with MMC. ICEBs1 excision in donor (16). The numbers below the cartoon of ICEBs1 indicate the mean fold-increase in mRNA levels in cells overexpressing rapI.…”

Section: Methodsmentioning

confidence: 99%

“…One of the potential att sites is in the 3Ј end of a tRNA gene, a common integration site for mobile elements (41). ICEBs1 contains int (previously ydcL) (16), encoding a putative -like integrase, immR (previously ydcN), encoding a putative bacteriophage-like immunity repressor with 50% amino acid similarity to the repressor of B. subtilis phage 105 (42,43), and seven genes similar to genes from other ICEs (16 (5). We used a PCR-based assay to detect products formed upon ICEBs1 excision (Fig.…”

Section: Identification Of a Mobile Genetic Element Regulated By Peptidementioning

confidence: 99%

“…We characterized a 20-kb ICE, ICEBs1 (16), in Bacillus subtilis and found that ICEBs1 excision and transfer is regulated by a secreted peptide encoded by ICEBs1.…”

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confidence: 99%

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Regulation of a Bacillus subtilis mobile genetic element by intercellular signaling and the global DNA damage response

Auchtung

Lee

Monson

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

246

523

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Horizontal gene transfer contributes to the evolution of bacterial species. Mobile genetic elements play an important role in horizontal gene transfer, and characterization of the regulation of these elements should provide insight into conditions that influence bacterial evolution. We characterized a mobile genetic element, ICEBs1, in the Gram-positive bacterium Bacillus subtilis and found that it is a functional integrative and conjugative element (ICE) capable of transferring to Bacillus and Listeria species. We identified two conditions that promote ICEBs1 transfer: conditions that induce the global DNA damage response and crowding by potential recipients that lack ICEBs1. Transfer of ICEBs1 into cells that already contain the element is inhibited by an intercellular signaling peptide encoded by ICEBs1. The dual regulation of ICEBs1 allows for passive propagation in the host cell until either the potential mating partners lacking ICEBs1 are present or the host cell is in distress.conjugation ͉ horizontal gene transfer ͉ quorum sensing ͉ peptide signaling ͉ DNA microarrays

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Section: Identification Of a Mobile Genetic Element Regulated By Peptidementioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Identification Of a Mobile Genetic Element Regulated By Peptidementioning

confidence: 99%

“…We characterized a 20-kb ICE, ICEBs1 (16), in Bacillus subtilis and found that ICEBs1 excision and transfer is regulated by a secreted peptide encoded by ICEBs1.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Regulation of a Bacillus subtilis mobile genetic element by intercellular signaling and the global DNA damage response

Auchtung

Lee

Monson

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

246

523

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“…ICESt3 is able to transfer between S. thermophilus strains as well as towards other lactic acid bacteria such as Enterococcus faecalis (Bellanger et al, 2009). The gene content and organization of the conjugation modules of ICESt3, ICEBs1 and Tn916 is highly similar although their conjugation proteins are distantly or very distantly related (Burrus et al, 2002b). The members of the ICESt1/ICESt3 family carry closely related conjugation modules found in various streptococci and harbour a peculiar regulation module.…”

Section: Introductionmentioning

confidence: 99%

Plasmid-like replication of a minimal streptococcal integrative and conjugative element

et al. 2016

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Integrative and conjugative elements (ICEs) are mobile genetic elements encoding their own excision from a replicon of their bacterial host, transfer by conjugation to a recipient bacterium and reintegration for maintenance. The conjugation, recombination and regulation modules of ICEs of the ICESt3 family are grouped together in a region called the ICE 'core region'. In addition to this core region, elements belonging to this family carry a highly variable region including cargo genes that could be involved in bacterial adaptation or in the maintenance of the element. Although ICEs are a major class of mobile elements through bacterial genomes, the functionality of an element encoding only its excision, transfer, integration and regulation has never been demonstrated experimentally. We engineered MiniICESt3, an artificial ICE derived from ICESt3, devoid of its cargo genes and thus only harbouring the core region. The functionality of this minimal element was assessed. MiniICESt3 was found to be able to excise at a rate of 3.1 %, transfer with a frequency of 1.0610 25 transconjugants per donor cell and stably maintain by site-specific integration into the 39 end of the fda gene, the same as ICESt3. Furthermore, MiniICESt3 was found in ,10 copies per chromosome, this multicopy state likely contributing to its stability for .100 generations even in the absence of selection. Therefore, although ICEs were primarily assumed to only replicate along with the chromosome, our results uncovered extrachromosomal rolling-circle replicating plasmid-like forms of MiniICESt3.

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UV Stress‐Responsive Genes Associated with Enterobacterial Integrative Conjugative Elements of the ICE SXT/R391 Group

Armshaw

Pembroke

2016

Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria

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The ICESt1 element of Streptococcus thermophilus belongs to a large family of integrative and conjugative elements that exchange modules and change their specificity of integration

Cited by 130 publications

References 62 publications

Regulation of a Bacillus subtilis mobile genetic element by intercellular signaling and the global DNA damage response

Regulation of a Bacillus subtilis mobile genetic element by intercellular signaling and the global DNA damage response

Plasmid-like replication of a minimal streptococcal integrative and conjugative element

UV Stress‐Responsive Genes Associated with Enterobacterial Integrative Conjugative Elements of the ICE SXT/R391 Group

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