The identification of a MYB transcription factor controlling anthocyanin biosynthesis regulation in Chrysanthemum flowers

Liu, Xincheng; Xiang, Lili; X, Yin; Grierson, Donald; Li, Fang; Chen, Kunsong

doi:10.1016/j.scienta.2015.08.018

“…Those MYB proteins with high similarities to EsMYB90 belong to the class of R2R3-MYB which have a conserved DNA-binding domain (R2 and R3 repeats) in the N-terminal and a variable C-terminal region [41,53,54]. The ANDV motif (marked by red A box in Fig.1A), a characteristic identifier for anthocyanin-promoting MYBs in dicots [10], existed in EsMYB90, AtMYB90, AtMYB75, AtMYB113, AtMYB114, AmROSEA1, StMYB113, EsMYBA1(AGT39060), VvMYBA1, MrMYB1, and FaMYB10, while the C-terminal-conserved motif KPRPR [S/T]F for Arabidopsis anthocyanin-promoting MYBs [25,36] (marked by blue B box in Fig.1A), which is required for interaction with R/B-like bHLH proteins [10,16].…”

Section: Database Mining Identifies Esmyb90 a Candidate Regulator Fomentioning

confidence: 99%

“…Chrysanthemum [10], grape hyacinth (Muscari armeniacum) [36], grapevine (Vitis vinifera) [37,38], chinese bayberry(Myrica rubra) [6,39], Epimedium sagittatum [40,41], poplar (Populus spp) [42] and potato (Solanum tuberosum L) [43]. In addition, some MYB genes are up-regulated under various stress conditions [15,17].…”

mentioning

confidence: 99%

Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

Qi

¹

,

Gu

²

,

Wang

³

et al. 2020

Preprint

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Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum . Result: Here we report the identification of an important anthocyanin biosynthesis regulator Es MYB90 from Eutrema salsugineum , which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that Es MYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by Es MYB90 in 35S : EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that Es MYB90 promoted expression of early ( PAL , CHS , and CHI ) and late ( DFR , ANS , and UFGT ) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S : EsMYB90 tobacco transgenic plants. Conclusions: Our results indicated that Es MYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants. Keywords : Anthocyanin, flavonoid, Eutrema salsugineum , R2R3 MYB transcription factor, Es MYB90, transcriptional regulation, anthocyanin biosynthesis genes.

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“…Those MYB proteins with high similarities to EsMYB90 belong to the class of R2R3-MYB which have a conserved DNA-binding domain (R2 and R3 repeats) in the N-terminal and a variable C-terminal region [41,53,54]. The ANDV motif (marked by red A box in Fig.1A), a characteristic identifier for anthocyanin-promoting MYBs in dicots [10], existed in EsMYB90, AtMYB90, AtMYB75, AtMYB113, AtMYB114, AmROSEA1, StMYB113, EsMYBA1(AGT39060), VvMYBA1, MrMYB1, and FaMYB10, while the C-terminal-conserved motif KPRPR [S/T]F for Arabidopsis anthocyanin-promoting MYBs [25,36] (marked by blue B box in Fig.1A), which is required for interaction with R/B-like bHLH proteins [10,16].…”

Section: Database Mining Identifies Esmyb90 a Candidate Regulator Fomentioning

confidence: 99%

“…Chrysanthemum [10], grape hyacinth (Muscari armeniacum) [36], grapevine (Vitis vinifera) [37,38], chinese bayberry(Myrica rubra) [6,39], Epimedium sagittatum [40,41], poplar (Populus spp) [42] and potato (Solanum tuberosum L) [43]. In addition, some MYB genes are up-regulated under various stress conditions [15,17].…”

mentioning

confidence: 99%

Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

Zhang

¹

,

Qi

²

,

Gu

³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum.Result: Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum, which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that EsMYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by EsMYB90 in 35S:EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants. Conclusions:Our results indicated that EsMYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants. BackgroundFlavonoids which are derivatives of the phenylpropanoid/flavonoid pathway mainly contain proanthocyanidins (PAs), anthocyanins and flavonols [1][2][3]. As important pigments, anthocyanins are responsible for red, purple, violet and blue colors in flowers, fruits, and leaves, which determine economic traits of crops and ornamental plants [4][5][6][7]. Anthocyanins are the end products of a specific branch in the phenylpropanoid/flavonoid biosynthesis pathway. Enzymes involved in anthocyanin biosynthesis have been extensively studied in many plant species [8]. Catalyzed by phenylalanine ammonia-lyase (PAL), the initial step of the flavonoid pathway is the conversion of phenylalanine into trans-cinnamic acid [9], while chalcone synthase (CHS) catalyzes the first committed step in the flavonoid biosynthesis to form naringenin chalcone. Chalcone isomerase (CHI) cyclizes chalcone to 4 form naringenin [8]. The naringenin is then converted into dihydrokaempferol (DHK) by flavanone 3 βhydroxylase (F3H). DHK is further hydroxylated to dihydroquercetin (DHQ) by flavonoid 3 'hydroxylase (F3'H), or to dihydromyricetin (DHM) by flavonoid 3',5'-hydroxylase (F3'5'H). Dihydroflavonol 4-reductase (DFR) converts DHQ into leucocyanidin, which is further converted into anthocyanidins by anthocyanidin synthase (ANS). Finally, UDP-glucose: flavonoid 3-Oglucosyltranferase (UFGT) catalyzes glycosylation of anthocyanidins to form anthocyanins [8,10-12]. MYB transcription factors play a central role in regulating expression of genes encoding major enzymes for anthocyanin biosynthesis via forming the transcriptional complex containing MYB-bHLH-WD40 (MBW) [1,13-15]. Expression...

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“…). In Chrysanthemum , the CmDFR promoter was activated more than 8‐fold by CmMYB6 alone and approximately 34‐fold by a combination of CmMYB6 and MrbHLH1 (from Myrica rubra ), as revealed by a dual luciferase assay (Liu et al, ). Functional studies showed that ectopic expression of GMYB10 in Gerbera hybrid leads to strongly enhanced accumulation of anthocyanin pigments (Laitinen et al, ), while mutations in GtMYB3 of Gentiana resulted in paler flower color (Nakatsuka et al, ).…”

Section: Introductionmentioning

confidence: 99%

Evolution and developmental genetics of floral display—A review of progress

Ma

¹

,

Zhang

²

,

Xiang

³

2017

J of Sytematics Evolution

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Angiosperms evolved a great diversity of ways to display their flowers for reproductive success by variation in floral color, size, shape, scent, arrangements, and flowering time. The various innovations in floral forms and the aggregation of flowers into different kinds of inflorescences can drive new ecological adaptations, speciation, and angiosperm diversification. Evolutionary developmental biology (evo-devo) seeks to uncover the developmental and genetic basis underlying morphological diversification. Advances in the developmental genetics of floral display have provided a foundation for insights into the genetic basis of floral and inflorescence evolution. A number of regulatory genes controlling floral and inflorescence development have been identified in model plants (e.g., Arabidopsis thaliana, Antirrhinum majus) using forward genetics and conserved functions of many of these genes across diverse non-model species have been revealed by reverse genetics. Gene-regulatory networks that mediated the developmental progresses of floral and inflorescence development have also been established in some plant species. Meanwhile, phylogeny-based comparative analysis of morphological and genetic character has enabled the identification of key evolutionary events that lead to morphological complexity and diversification. Here we review the recent progress on evo-devo studies of floral display including floral symmetry, petal fusion, floral color, floral scent, and inflorescences. We also review the molecular genetic approaches applied to plant evo-devo studies and highlight the future directions of evo-devo.

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The identification of a MYB transcription factor controlling anthocyanin biosynthesis regulation in Chrysanthemum flowers

Cited by 50 publications

References 41 publications

Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

Evolution and developmental genetics of floral display—A review of progress

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