The immune adjuvant response of polysaccharides from Atractylodis macrocephalae Koidz in chickens vaccinated against Newcastle disease (ND)

Zhao, Xiaona; Sun, Wenjing; Zhang, Shijie; Meng, Guangju; Qi, Chenze; Fan, Wentao; Wang, Yuge; Liu, Jianzhu

doi:10.1016/j.carbpol.2016.01.013

Cited by 42 publications

(15 citation statements)

References 26 publications

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“…Wang et al [27] have showed that the polysaccharide from Paulownia fortunei flowers can promote cellular and humoral immunity in chickens vaccinated with ND vaccine through increasing the levels of IL-2 and IFN-γ, duodenal sIgA content as well. Additionally, Zhao et al [28] have reported that polysaccharides from the rhizome of Atractylodis macrocephalae Koidz (RAMPS) could be an immunopotentiator in chickens vaccinated with ND vaccine, which enhances serum HI antibody titer and improves the percentages of CD4 + and CD8 + T cells. In our previous study, we also reported that Ganoderma lucidum polysaccharide (GLP) could be an immunomodulator in chickens vaccinated with ND vaccine through promoting lymphocyte proliferation and elevating serum antibody titer [6].…”

Section: Discussionmentioning

confidence: 99%

The enhanced immunological activity of Paulownia tomentosa flower polysaccharide on Newcastle disease vaccine in chicken

Yang

Zhang

Xu³

et al. 2019

Bioscience Reports

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The extracts of Paulownia tomentosa (P. tomentosa) exhibit multiple pharmacological activities. In the present study, P. tomentosa flower polysaccharides (PTFP) were extracted by water decoction and ethanol precipitation, and the immunologic modulations of PTFP against Newcastle disease (ND) vaccine was investigated in chickens. The results showed that in a certain range of concentrations, PTFP treatment can dose-dependently enhance lymphocyte proliferation. Then, 280 14-days-old chickens were randomly divided into seven groups, and vaccinated with ND vaccine except blank control (BC) group. At the first vaccination, chickens were orally administrated with PTFP at concentration ranging from 0 to 50 mg/kg once a day for 3 successive days, and the BC group was treated with physiological saline. The lymphocyte proliferation rate, serum antibody titer, and levels of interferon-γ (IFN-γ) were respectively measured on 7, 14, 21, and 28 days after the first vaccination. The results showed that PTFP at the suitable doses could significantly promote lymphocyte proliferation, enhance serum antibody titer, and improve serum IFN-γ concentrations. Taken together, these data indicated that PTFP could improve the immune efficacy against ND vaccine in chickens, and could be as the candidate of a new-type immune adjuvant.

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Section: Discussionmentioning

confidence: 99%

The enhanced immunological activity of Paulownia tomentosa flower polysaccharide on Newcastle disease vaccine in chicken

Yang

Zhang

Xu³

et al. 2019

Bioscience Reports

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“…Many polysaccharides from plant, bacterial, yeast and synthetic sources can act as pathogen-associated molecular patterns (PAMPs) and recognize pattern recognition receptors (PRRs) on immune cells, followed by regulating immunity [6][7][8][9]. Polysaccharides are safe, and biodegradable, with no tissue deposition [10].…”

Section: Introductionmentioning

confidence: 99%

Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner

et al. 2020

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There is an urgent need for the development of new, improved vaccine adjuvants against T. spiralis infection. Polysaccharides are effective, safe, and biodegradable as adjuvant. In our study, we first observed the protective efficacy of lentinan as adjuvant against helminth T. spiralis infection. Recombinant T. spiralis Serpin (rTs-Serpin) immunoscreened from a cDNA library of T. spiralis, as a vaccine, protect host against Trichinella infection. The reduction rate of helminth burden of rTs-Serpin+lentinan-immunized mice was significantly increased compared with rTs-Serpin+FCA-immunized mice. rTs-Serpin+lentinan induced IgG1-dominant immune response and higher levels of IFN-γ and IL-4. rTs-Serpin+lentinan displayed a lower reduction rate of parasite burden in NLRP3-/mice than that in WT mice and lower level of IgG1 than that in WT mice. The level of IL-4, but not IFN-γ, from NLRP3-/mice immunized by rTs-Serpin+lentinan was significantly lower than that from WT mice, suggesting that NLRP3 is associated with rTs-Serpin+lentinan-triggering Th2 protective immunity against T. spiralis infection. In summary, we revealed that lentinan was a novel adjuvant against T. spiralis infection via NLRP3. NLRP3 therefore represents an important target for adjuvant discovery and the control of T. spiralis infection.

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“…Many Chinese herbal polysaccharides have obvious advantages in improving humoral immunity and cellular immunity, such as Cyrtomium macrophyllum [4], Cassia obtusifolia [5], Atractylodes macrocephala Koidz (RAMPS) [6], RAMPStp and RAMPS60c [7]. Numerous Chinese herbal polysaccharides can also enhance the mucosal immunity of animals.…”

Section: Introductionmentioning

confidence: 99%

Purification, characterization and in vitro and in vivo immune enhancement of polysaccharides from mulberry leaves

et al. 2019

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Mulberry leaf polysaccharide (MLP) was extracted and purified by DEAE-52 cellulose and Sephadex G-100 column chromatography to afford two major purified polysaccharides (MLP-1 and MLP-2). The purified polysaccharides were characterized, and their immune-enhancing properties were investigated. MLP-1 had a molecular weight of 9.31×104 Da and was composed of mannose, rhamnose, glucose, galactose, xylose, and arabinose in a molar ratio of 0.71:1.00:2.76:1.13:3.70:2.81. The molecular weight of MLP-2 was 2.22×106 Da, and its monosaccharide constituents were mannose, rhamnose, glucose, galactose, and arabinose in a molar ratio of 1.31:8.45:6.94:1.00:11.96. Infrared spectroscopy showed that each MLP had a typical absorption peak characteristic of sugars, and ultraviolet (UV) spectroscopy showed that neither MLP contained nucleic acid or protein components. Then, the abilities of these polysaccharides to stimulate spleen lymphocyte proliferation in mice in vitro were compared by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. MLP-2 was more effective than MLP-1; therefore, MLP-2 was chosen for the study of its immune-enhancing effects in vivo. For the in vivo experiments, 14-day-old chickens immunized with Newcastle disease (ND) vaccine were orally administered MLP-2, and Astragalus polysaccharide (APS) was used as the control. Each chicken was orally administered 4 mg or 8 mg of MLP-2 for seven consecutive days starting three days before ND vaccine immunization. MLP-2 significantly improved the ND serum antibody titer and interleukin-2 (IL-2), interferon-γ (IFN-γ) and immunoglobulin A (sIgA) concentrations in tracheal and jejunal wash fluids, and increasing numbers of immune globulin A-positive (IgA+) cells in cecal tonsils and increased body weight. These results indicated that MLP-2 could significantly enhance immune activity and could therefore be utilized as an immunopotentiator drug candidate.

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The immune adjuvant response of polysaccharides from Atractylodis macrocephalae Koidz in chickens vaccinated against Newcastle disease (ND)

Cited by 42 publications

References 26 publications

The enhanced immunological activity of Paulownia tomentosa flower polysaccharide on Newcastle disease vaccine in chicken

The enhanced immunological activity of Paulownia tomentosa flower polysaccharide on Newcastle disease vaccine in chicken

Lentinan improved the efficacy of vaccine against Trichinella spiralis in an NLRP3 dependent manner

Purification, characterization and in vitro and in vivo immune enhancement of polysaccharides from mulberry leaves

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