The Immune Response of Normal, Irradiated and Thymectomized Mice to Fowl Immunoglobulin G as Detected by a Hemolytic Plaque Technique

Miller, J. F. A. P.; Warner, N. L.

doi:10.1159/000230395

Cited by 63 publications

(36 citation statements)

References 14 publications

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“…The peak 19S and 7S anti-F3,G PFC response in F~G-tolerant mice was of the same order of magnitude as that occurring after challenging (TxBM) mice with F3'G AP (Table IV). The low response of these thynlectomized animals is in accordance with previous studies indicating thymus-dependency of the humoral antibody respons e of mice to FTG (13). <2-4 (7) 32-1024 (11) 32-1024 (12) * No.…”

Section: Induction Of Tolerance To F'yg In Adultsupporting

confidence: 90%

“…, and horse erythrocytes (HRBC) were obtained as described elsewhere (11)(12)(13). Human gamma globulin (HGG) was supplied from the Commonwealth Serum Laboratories, Melbourne, Australia, in 10-ml vials containing 160 mg protein/ml.…”

Section: Antigens--fowl Immunoglobulin G (F3'g) Sheep Erythrocytes mentioning

confidence: 99%

“…The mice were used 2 wk later. Assays for tolerance were performed in some mice after challenging with 500 #g of F3'G AP either by measuring the serum-binding capacity using radioiodinated F')'G (18) or by determining splenic anti-F3'G plaque-forming cells (PFC) (13).…”

Section: Antigens--fowl Immunoglobulin G (F3'g) Sheep Erythrocytes mentioning

confidence: 99%

“…The immunoglobulin G-containing fraction was separated from the serum by the technique described previously (13) and the fowl anti-mouse lymphocyte globulin (FALG) fraction titrated for its lymphoagglutinating activity and stored in small aliquots at --20°C; (e) rabbit anti-mouse lymphocyte globulin (RALG) and normal rabbit globulin were prepared as described previously (19) ; (f) anti-0 C3H serum (20).…”

Section: Antigens--fowl Immunoglobulin G (F3'g) Sheep Erythrocytes mentioning

confidence: 99%

“…The titer of mouse anti-FTG serum was measured either by its hemagglutination activity against SRBC coated with FTG in the form of fowl anti-SRBC imrnunoglobulin G, as described elsewhere (13) or by microprecipitation assay with F3,G-125I, in a manner analogous to the previously described method of testing for anti-immunoglobulin sara (18). The antibody activity of the FALG and RALG preparations were titrated by the techniques of lymphocytotoxicity and lymphoagglutination (22).…”

Section: Antigens--fowl Immunoglobulin G (F3'g) Sheep Erythrocytes mentioning

confidence: 99%

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Cell-to-Cell Interaction in the Immune Response

Miller

Sprent

Basten

et al. 1971

The Journal of Experimental Medicine

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Experiments were designed to test the possibility that thymus-derived (T) cells cooperate with nonthymus derived (B) cells in antibody responses by acting as passive carriers of antigen. Thoracic duct lymphocytes (TDL) from fowl γG-tolerant mice were incubated in vitro with fowl anti-mouse lymphocyte globulin (FALG), which was shown not to be immunosuppressive in mice. On transfer into adult thymectomized, irradiated, and marrow protected (TxBM) hosts together with a control antigen, horse RBC, a response to horse RBC but not to fowl γG was obtained. By contrast, TxBM recipients of nontolerant, FALG-coated TDL responded to both antigens and the antibody-forming cells were shown to be derived from the host, not from the injected TDL. These findings suggested that, under the conditions of the experiment, triggering of unprimed B cells in the spleens of TxBM hosts was not achieved with antigen-coated tolerant lymphocytes. Another model utilized the ability of B cells to bind antibody-antigen complexes. Spleen cells from TxBM mice, incubated in vitro with anti-fowl γG-fowl γG·NIP, were injected with or without normal TDL (a source of T cells) into irradiated hosts. Only mice given both cell types could produce an anti-NIP antibody response. In a further experiment, spleen cells from HGG·NIP-primed mice were injected together with NIP-coated B cells (prepared as above) into irradiated hosts. A substantial anti-NIP antibody response occurred. If, however, the T cells in the spleens of HGG·NIP-primed mice were eliminated by treatment with anti-θ serum and complement, the NIP response was abolished. It was concluded that antigen-coated B cells could not substitute for T cells either in the primary or secondary response. Treatment of T cells from unprimed or primed mice with mitomycin C impaired their capacity to collaborate with B cells on transfer into irradiated hosts. Taken together these findings suggest that before collaboration can take place T cells must be activated by antigen to differentiate and in so doing may produce some factor essential for triggering of B cells.

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Section: Induction Of Tolerance To F'yg In Adultsupporting

confidence: 90%

Section: Antigens--fowl Immunoglobulin G (F3'g) Sheep Erythrocytes mentioning

confidence: 99%

Section: Antigens--fowl Immunoglobulin G (F3'g) Sheep Erythrocytes mentioning

confidence: 99%

Section: Antigens--fowl Immunoglobulin G (F3'g) Sheep Erythrocytes mentioning

confidence: 99%

Section: Antigens--fowl Immunoglobulin G (F3'g) Sheep Erythrocytes mentioning

confidence: 99%

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Cell-to-Cell Interaction in the Immune Response

Miller

Sprent

Basten

et al. 1971

The Journal of Experimental Medicine

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Induction of immunity and tolerance in vitro by hapten protein conjugates. II. Carrier independence of the response to dinitrophenylated polymerized flagellin

Feldmann

1972

Eur J Immunol

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Cell interactions in the immune response in vitro. I. Metabolic activities of T cells in a collaborative antibody response

Feldmann

Basten

1972

Eur J Immunol

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Cell collaboration between thymus-derived (T) and non-thymus-derived (B) lymphocytes in induction of the response to SRC was investigated in an in vitro test system. Different populations of T cells were found t o vary in helper activity depending on their degree of differentiation following exposure to antigen. Activated thymus cells obtained from the spleen of irradiated mice injected with syngeneic thymocytes and SRC proved the most effective, followed by primed T cells and then unprimed T cells. The interaction between T cells and B cells as measured here was specific for both classes of lymphocytes.The mechanism of T cell action in the collaborative response was analyzed by treating these populations of T cells with agents which became firmly cell bound and could selectively inhibit different aspects of cell metabolism.

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The Immune Response of Normal, Irradiated and Thymectomized Mice to Fowl Immunoglobulin G as Detected by a Hemolytic Plaque Technique

Cited by 63 publications

References 14 publications

Cell-to-Cell Interaction in the Immune Response

Cell-to-Cell Interaction in the Immune Response

Induction of immunity and tolerance in vitro by hapten protein conjugates. II. Carrier independence of the response to dinitrophenylated polymerized flagellin

Cell interactions in the immune response in vitro. I. Metabolic activities of T cells in a collaborative antibody response

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