The immune response of the brown trout Salmo trutta to lipopolysaccharide

Abstract

Brown trout produced high molecular weight, thermostable, dithiothreitol sensitive, nonprecipitating, complement-fixing antibodies and agglutinins to lipopolysaccharides after intramuscular injection with adjuvant. Antibodies were first detected on Day 14 and reached maximum titres after 56 to 63 days when a single injection was given. When either a second or a third injection was administered maximum titres occurred 34 to 40 days after the injection. After each injection the titres increased significantly, and the protein concentration of the sera was significantly decreased. In cellulose acetate electrophoresis experiments those bands which migrated in thep-to y-globulin regions were increased.Antibody-secreting and antigen-binding cells were detected on Days 8 and 4 respectively and maxima were reached between Day 16 and Day 18. The number of cells per lo6 lymphoid cells was higher in the spleen than in the kidney.

Distribution of intravenously and perorally administeredAeromonas salmonicidalipopolysaccharide in Atlantic salmon,Salmo salarL.

“…Also, the so-called O-antigens of LPS molecules are important surface antigens, (Dooley et al, 1985;Rockey et al, 1991). The immune response to this molecule has been investigated in fish (Ingram et al, 1980;Bøgwald et al, 1991Bøgwald et al, , 1992. In addition, LPS may stimulate leukocytes in vitro (Clem et al, 1985;Zeliko# et al, 1991;Secombes, 1994;Solem et al, 1995).…”

Bacterial lipopolysaccharide (LPS) and interleukin 1 (IL-1) exert multiple physiological effects in the tilapia Oreochromis mossambicus (Teleostei)

“…The pituitary is unlikely to be the sole target of neuroimmunological regulations in fish, in view of the complexity of the effects exerted by LPS and cytokines as IL-1 in higher vertebrates. In fish, bacterial infections, as well as administration of LPS, have manifold immunological (Ingram and Alexander 1980), physio logical (Wakabayashi and Iwado 1985;Speare et al 1991), endocrinological (Wedemeyer 1969;White and Fletcher 1985), and histopathological (Walters and Plumb 1980) consequences. To substantiate the funda mental impact of immuno-endocrine interactions, we therefore investigated the effects of IL-1 and LPS ad ministration on the pituitary-interrenal axis and sev eral of its targets in fish.…”

Immune response of channel catfish to ipopolysaccharide and whole cell Edward-siella ictaluri vaccines

“…Studies on the immune response of fish have employed many antigens including particulate (virus), cellular (bacteria) and molecular (proteins, carbohy-MATERIALS AND METHODS drates, and haptens) substances. In a few cases, lipopolysaccharides (LPS) or endotoxins from the bacEdwardsieLla i c t a l w (Hawke et al 1981) (ATCC terial cell wall have been used to stimulate an immune 33202) was reconstituted from lyophilized pellets and response in fish (Cisar & Fryer 1974, Paterson & Fryer grown on brain-heart infusion (BHI) agar plates at 1974, Busch 1978, Ingram & Alexander 1980. Enteric 30°C.…”