The Immunohistochemical Evaluation of Estrogen Receptor-α and Progesterone Receptors of Normal, Hyperplastic, and Neoplastic Endometrium in 88 Pet Rabbits

Asakawa, Makoto; Goldschmidt, Michael H.; Une, Yumi; Nomura, Yasuo

doi:10.1354/vp.45-2-217

Cited by 45 publications

(30 citation statements)

References 18 publications

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“…Increased ER-a and PR expression were found to be correlated with a better prognosis in canine mammary tumours [25,26]. In rabbits with uterine adenocarcinoma, however, expression of ER-a and PR did not reflect prognosis, despite varying hormone receptor expression playing a role in the development of two different types of tumours, papillary or tubular [27]. The discrepancies identified suggest that the usefulness of these receptors as markers may be species and/or tumour type dependent.…”

Section: Aetiology Of Urogenital Carcinoma In the California Sea Lionmentioning

confidence: 94%

Common cancer in a wild animal: the California sea lion ( Zalophus californianus ) as an emerging model for carcinogenesis

Browning

Gulland

Hammond

et al. 2015

Phil. Trans. R. Soc. B

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Naturally occurring cancers in non-laboratory species have great potential in helping to decipher the often complex causes of neoplasia. Wild animal models could add substantially to our understanding of carcinogenesis, particularly of genetic and environmental interactions, but they are currently underutilized. Studying neoplasia in wild animals is difficult and especially challenging in marine mammals owing to their inaccessibility, lack of exposure history, and ethical, logistical and legal limits on experimentation. Despite this, California sea lions ( Zalophus californianus ) offer an opportunity to investigate risk factors for neoplasia development that have implications for terrestrial mammals and humans who share much of their environment and diet. A relatively accessible California sea lion population on the west coast of the USA has a high prevalence of urogenital carcinoma and is regularly sampled during veterinary care in wildlife rehabilitation centres. Collaborative studies have revealed that genotype, persistent organic pollutants and a herpesvirus are all associated with this cancer. This paper reviews research to date on the epidemiology and pathogenesis of urogenital carcinoma in this species, and presents the California sea lion as an important and currently underexploited wild animal model of carcinogenesis.

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Section: Aetiology Of Urogenital Carcinoma In the California Sea Lionmentioning

confidence: 94%

Common cancer in a wild animal: the California sea lion ( Zalophus californianus ) as an emerging model for carcinogenesis

Browning

Gulland

Hammond

et al. 2015

Phil. Trans. R. Soc. B

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“…Ten slides from each animal group were evaluated for p16 and ERα expression by two blinded independent evaluators. The area labeled with specific antibody and the percentage of cells positive for the antigen were calculated using published procedures [22]. The evaluation of immunostaining of ERα and p16 was performed on both the stromal and epithelial components, but only statistical analysis of the stromal regions were reported.…”

Section: Methodsmentioning

confidence: 99%

Exercise Training Prevents Endometrial Hyperplasia and Biomarkers for Endometrial Cancer in Rat Model of Type 1 Diabetes

et al. 2010

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BackgroundEndometrial cancer is one of the most common types of gynecologic cancers. The ability of exercise to reduce the risk of endometrial cancer in women with type 2 diabetes has been established, but no studies have examined this link in type 1 diabetes.A randomized, controlled animal study was designed using a standard rat model of type 1 diabetes. The goal of this study was to investigate the ability of exercise to prevent increased levels of endometrial cancer biomarkers, estrogen receptor (ERα) and p16, and endometrial hyperplasia associated with diabetes.MethodsForty female rats were randomized into four groups: sedentary control, exercise control, sedentary or exercised diabetic. Diabetes was induced by alloxan injection. A 4-week treadmill training program was initiated with the development of diabetes. Endometrial tissues were evaluated for hyperplasia and ERα and p16 levels and subcellular localization using microscopy.ResultsSevere diabetes lead to hyperplasia in the endometrial tissue in 70% of sedentary diabetic rats. Exercise-trained diabetic rats and the non-diabetic rats displayed no hyperplasia. The expression of ERα increased significantly (p < 0.02) while the expression level of p16 decreased significantly (p < 0.04) in the diabetic sedentary group compared to the non-diabetic groups. Exercise training led to a reversal in the percentage of p16 and ERα positive cells in diabetic rats.ConclusionsSevere diabetes leads to hyperplasia of the endometrial tissue and increased ERα levels and decreased p16 levels in rats, which can be prevented with aerobic exercise.KeywordsDiabetes; Estrogen receptor alpha; P16; Endometrial hyperplasia; Endometrial cancer; Exercise

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“…Variations in the immunoreactivity of smooth muscle tumors to SMA and Desmin might be related to the degree of differentiation of each tumor (Cooper and Valentine, 2002). A variety of exogenous and endogenous hormones can influence the mitotic index and the development of tumor cell necrosis (Asakawa et al, 2008).…”

Section: Immunohistochemical Evaluationmentioning

confidence: 99%

Protecting Role of Selenium on the Cytotoxic Effect of Profenofos on Rabbit Fertility

Abdel-Rahman¹,

El-Metwally²,

El-Hallawany³

et al. 2017

AJVS

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Key words:Profenofos -Histopathology-Caspase-3 -Biochemical-HormonesAntioxidants-Reproductive toxicity -Selenium-Liverkidneys -Rabbits.Profenofos is an Organophosphorous compound and acaricide used in Egypt with cumulative toxic effects causing reproductive dysfunction and increase in reactive oxygen species production. Protective and anti-oxidant effects of selenium can improve the activity of radical scavenger. So, the aim of this study was to elucidate the protective effects of Selenium against reproductive toxicity induced by Profenofos in male and female New Zealand rabbits (n=18). Three experimental groups (3 males and 3 females per each group) receiving a combination of Profenofos (70 mg/ kg b. w.) and/or selenium in drinking water (25 mg/ kg b. w.) for 90-day was divided as follows: non treated (control), Profenofos alone and Profenofos + selenium group. Profenofos caused a decrease in body and organs weights compared to control group. It had a fetotoxic effect resulting in a significant reduction in pregnancy rate and the number of viable feti in treated animals. Also, Profenofos caused a significant elevation in ALT, AST, MDA, urea and creatinine, while a significant decrease was noticed in SOD, GSH, testosterone, estrogen and progesterone in all treated rabbits compared to control group. Concomitant administration of selenium with Profenofos alleviated the induced alterations. In addition to, histopathological results revealed that testes, epididymis, ovary and uterus alterations represented by degenerative and inflammatory changes as a result of Profenofos exposure. Profenofos had a carcinogenic effect on uterus as leiomyoma with rhabdomyosarcoma which gave positive immunoreactivity with Desmin and Smooth muscle actin. Also, Profenofos caused histopathological and histochemical effects in liver and kidney tissues. There was intensive positive immunoreactivity for caspase-3 (dark brown granules) was observed in the cytoplasm of apoptotic testicular, ovarian and uterine cells that indicating the severity of Profenofos toxicity. On the other hand, examined tissues of Profenofos in combination with selenium treated group showed apparent reduction of caspase-3 immunoreactivity to be more or less similar to the control group. In conclusion, selenium is a valuable cytoprotective which reduced the oxidative stress toxicity induced by Profenofos in the reproductive system of male and female as well as liver and kidney albino rabbits.

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The Immunohistochemical Evaluation of Estrogen Receptor-α and Progesterone Receptors of Normal, Hyperplastic, and Neoplastic Endometrium in 88 Pet Rabbits

Cited by 45 publications

References 18 publications

Common cancer in a wild animal: the California sea lion ( Zalophus californianus ) as an emerging model for carcinogenesis

Common cancer in a wild animal: the California sea lion ( Zalophus californianus ) as an emerging model for carcinogenesis

Exercise Training Prevents Endometrial Hyperplasia and Biomarkers for Endometrial Cancer in Rat Model of Type 1 Diabetes

Protecting Role of Selenium on the Cytotoxic Effect of Profenofos on Rabbit Fertility

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