The immunohistochemical expression of p21^WAF1/Cip1 and Proliferating cell nuclear antigen in laryngeal squamous cell carcinomas

Abstract

The purpose of this study was to assess the significance of the expression of p21WAF1/Cip1 and proliferating cell nuclear antigen (PCNA) in human laryngeal squamous cell carcinomas. Forty-eight patients (25 supraglottic, 23 glottic) who had undergone operations between 1998 and 2003 were included in this study. The Envision immunohistochemistry method was utilized to stain these tissue specimens. The results showed that the immunostaining of p21WAF1/Cip1 do not show any correlation with differentiation, N stage, metastasis, survival, recurrence or the laryngeal site of involvement. A significant inverse correlation was detected between p21WAF1/Cip1 and the T stage. In contrast, the percentage of PCNA labelled cells showed a significant correlation with the T stage, but not with other clinicopathological parameters. There were differences in the expression of p21WAF1/Cip1 and PCNA between the supraglottic and the glottic carcinomas. In conclusion, our findings suggest that p21WAF1/Cip1 and PCNA may play an important role in the progression of carcinoma of the supraglottic larynx.

Distinct Biomarker Profiles and Clinical Characteristics in T1‐T2 Glottic and Supraglottic Carcinomas

“…From an epidemiological perspective, male gender was significantly more dominant in the glottic than in the supraglottic LSCC patients. This finding is in concordance with other studies, 28–29 whereas in several studies gender was not related with tumor sublocalization 30–31 . Geographical differences in the use of alcohol between both sexes are known confounders 32 .…”

“…This finding is in concordance with other studies, 28,29 whereas in several studies gender was not related with tumor sublocalization. 30,31 Geographical differences in the use of alcohol between both sexes are known confounders. 32 Moreover, compared to the (sub)glottis, the supraglottis is exposed to a relatively higher degree to ingested agents and to a lesser degree to inhaled agents, 33 suggesting that etiological factors, like the use of alcohol and tobacco, might have distinguishable epidemiological effects.…”

Detection and significance of human papillomavirus, CDKN2A(p16) and CDKN1A(p21) expression in squamous cell carcinoma of the larynx

“…However, previous studies of CDKN1A(p21) in laryngeal carcinomas lack corresponding data on CDKN2A(p16) or HPV status (33–36). Ours is the first study to compare CDKN1A(p21) with CDKN2A(p16) and HPV status in laryngeal squamous cell carcinoma.…”

Transcriptional activities of histone H3, cyclin D1 and claudin 7 encoding genes in laryngeal cancer

“…Due to the occurrence of genetic changes prior to phenotypic consequences, new techniques of molecular biology such as real time RT-PCR or DNA microarray are reasonable to apply as diagnostic approaches in cancer. Several factors like p53, Ki67, PCNA [14][15][16][17][18][19][20] were previously discussed as potential laryngeal cancer markers. In the current work, real-time QRT-PCR was used to evaluate the expression level of histone H3, cyclin D1 and claudin 7 genes in LSCC.…”