The Immunohistochemical Staining of Vimentin and E-Cadherin in Bladder Cancer Patients Infected with Hepatitis C Virus

Ismael, May K.; Qaddoori, Yasir Basim; Sha'ban, Munirah; Al-Rubaii, Bahaa Abdullah Laftaah

doi:10.22207/jpam.17.2.30

Cited by 2 publications

(2 citation statements)

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“…As is widely recognised, the Polymerase Chain Reaction (PCR) has been employed in various medical domains pertaining to the identification of clinical pathogenesis associated with infectious diseases and other perilous genetic conditions, such as cancer. [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38]…”

Section: Discussionmentioning

confidence: 99%

Detection of Cytomegalovirus in Patients with Inflammatory Bowel Diseases in Iraq

Ahmed Majeed

2023

JCD

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Inflammatory bowel diseases (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), are chronic inflammatory disorders of the gastrointestinal tract caused by interactions between genetic, environmental, immunological, and microbial factors.Objective: To study the association of Cytomegalovirus in a sample of Iraqi patients with inflammatory bowel diseases Methodology: The study included 160 patients, of which, 80 were diagnosed with IBD by a specialist in gastroenterology and 80 were apparently healthy subjects. Stool samples were collected from all participants and were prepared for detection of CMV PP65 by ELISA. The molecular method (real-time PCR) was used to detect the DNA of each pathogen.Results: Twenty-five patients with IBD had a significantly high level (8.44 pg/ml) of CMV as compared to the control group. The results showed that there was no significant correlation between CMV PP65 and the signs and symptoms of IBD patients (smoking, UC severity, bloody diarrhoea, abdominal pain, biological therapy, behaviour, or CD location). CMV real-time copy quantity was significantly correlated with smoking, bloody diarrhoea, abdominal discomfort, and biological therapy, but not with UC extent, behaviour, or CD location, as determined by molecular real-time PCR. Conclusion:There is a possible role of CMV in the pathogenesis of IBD in patients in Iraq. The biological treatment given to patients with IBD seems to reduce the CMV level.

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Section: Discussionmentioning

confidence: 99%

Detection of Cytomegalovirus in Patients with Inflammatory Bowel Diseases in Iraq

Ahmed Majeed

2023

JCD

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“…It is linked to the absence of the epithelial brow and the presence of mesenchymal facial hair [7] . Adherens junctions and desmosomes play critical roles in maintaining cell and tissue integrity by mediating strong adhesion between adjacent epithelial cells [8] .…”

Section: Introductionmentioning

confidence: 99%

Estimation of gene expression level of CDH1 as a predisposing factor for metastasis in localized and locally advanced and metastatic breast cancer Iraqi patients

Abdallah,

Aziz,

Alsammarraie

2023

Trends Immunother.

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Breast cancer (BC) is a prevalent malignancy among women, ranking as the second most commonly diagnosed cancer globally. Notably, a substantial proportion of breast cancer-related fatalities, up to 90 percent, are attributed to the development of distant organ metastases. While Cadherin1 (CDH1) has conventionally been considered a tumor-suppressor gene in cancer research, recent investigations have unequivocally revealed that both CDH1 and its encoded E-cadherin exhibit oncogenic characteristics. The primary focus of this case-control study is to ascertain the involvement of the CDH1 gene in a specific subset of Iraqi female patients. A total of ninety patients sought diagnosis and treatment at the Oncology Teaching Hospital in Medical City and the Oncology Unit at Al-Yarmouk Teaching Hospital in Baghdad. In addition, we included 30 apparently healthy individuals as blood control subjects and another 30 women with benign breast tumors as tissue control subjects for the study. In the initial phase of the study, we conducted a serological analysis using enzyme-linked immunosorbent assay (ELISA) to detect the concentration of E-cadherin in serum samples from two groups of BC patients. The group with locally advanced and metastatic BC exhibited a significantly higher E-cadherin concentration (963.4 ± 89.8 pg/mL) compared to the group with localized BC. In the second part of the research, qRT-PCR was performed to analyze the expression of the CDH1 gene across all sample types. CDH1 was shown to have the greatest fold expression (2.550 ± 0.164) in cases with locally progressed and metastatic BC. Compared to the seemingly healthy control group, the fold expression for localized BC was 1.456 ± 0.055, and for malignant tissue it was 1.886 ± 0.08621. In conclusion, this study provides compelling evidence supporting CDH1 as an oncogene in BC. The significance of CDH1 in BC tumorigenesis underscores its potential for the development of novel detection biomarkers and targeted therapeutic approaches for BC treatment. Notably, CDH1 exhibited elevated expression levels in BC tissues and demonstrated an association with an unfavorable distant metastasis-free survival outcome.

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The Immunohistochemical Staining of Vimentin and E-Cadherin in Bladder Cancer Patients Infected with Hepatitis C Virus

Cited by 2 publications

References 61 publications

Detection of Cytomegalovirus in Patients with Inflammatory Bowel Diseases in Iraq

Detection of Cytomegalovirus in Patients with Inflammatory Bowel Diseases in Iraq

Estimation of gene expression level of CDH1 as a predisposing factor for metastasis in localized and locally advanced and metastatic breast cancer Iraqi patients

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