The immunological effects against invasive aspergillosis disease on inbred mice after the dietary intake of honey varieties with the determination of diastase and invertase enzyme activities

Burn wounds need enhanced healing by using some workable agent for better treatments. The current work aimed to make a comparison between four types of honey and vaseline in ointment forms in-vivo using experimental rats. Several parameters have been evaluated such as wound epithelialization and vascular endothelial growth factor expression (VEGF) rates for skins. Some biochemical parameters such as nitrites (NO2) and fluorescence recovery after photobleaching (FRAP) were detected for serum. TNF-α immune response and hydroxyproline contents were also determined colorimetrically. Microbial infection of the burns was examined against Pseudomonas aeruginosa and Staphylococcus aureus strains. The wound epithelialization rate among animal groups after 15 days of treatment ranged from 52.13% to 89.58%. The highest VEGF expression rate was achieved by using the ointment formed by side honey (SIH) at 9.21% followed by pumpkin honey (PUH) at 8.54%, moringa honey (MOH) at 8.11%, and nigellasativa honey (NSH) as 7.02%, while vaseline group reported 5.22% VEGF expression. The ointment formed by the NSH rat group detected the highest NO2 at the end of the experimental work to reach (0.07 μmol/L) followed by SIH (0.06 μmol/L), while MOH and PUH groups reported equal values (0.05 μmol/L). The FRAP values varied in rat groups from 735.19 μmol TE/g (SIH) to 877.89 μmol TE/g (NSH). NSH reported the highest value for TNF-α immune response at 589.47 U/mL followed by SIH at 560.89 U/mL, while MOH reported the lowest value at 489.58 U/mL. For the microbial infections, the MOH group had the lowest zone of inhibition 5.18 mm, while the vaseline group reported the highest 19.88 mm against Pseudomonas aeruginosa. It was observed that the highest zone of inhibition (19.22 mm) against Staphylococcus aureus was for the vaseline group, while the lowest zone of inhibition (5.33 mm) against Staphylococcus aureus was for SIH. All honey varieties, especially MOH had better results for NO2, TNF-α immune response, and successfully inhibited microbial infections in-vivo.

Healing processes of burn wounds with honey and vaseline as ointment forms: An in-vivo study in Wistar rats

Baakdah,

Ashi,

Almaghrabi

et al. 2023

Examining the pancreatic regenerative potential of Nigella sativa in diabetic rats: Evaluation of histological changes and transcription factors pdx-1 and neurog-3 levels

Alaryani

2024

Pancreatic β cell neogenesis and proliferation represent promising avenues for diabetes management. Nigella sativa, known for its potent anti-inflammatory and antioxidant properties, has demonstrated efficacy in promoting regeneration across various organ systems. This study investigates the potential of Nigella sativa in pancreatic regeneration by examining pancreatic histology in addition, the expression levels of the key transcription factors, comprising NEUROG-3, PDX-1, INS-1, and INS-2. Twenty-four rats were categorized in 4sets that is negative control, positive control, normal, and treatment. Diabetes was prompted through intraperitoneal administration of alloxan hydrate. After 21 days of diabetes induction, the treatment set was applied with N. sativa extract, the positive control set was administered Glibenclamide, while the negative control group received no treatment. Parameters such as biochemical markers, oxidative stress markers, pancreatic islet architecture changes, and the expression levels of the NEUROG-3, PDX-1, INS-1, and INS-2 were assessed. Data analysis, conducted through DMR Test and ANOVA with a 5% threshold of significance, revealed significant insights into the consequence of N. sativa on the parameters under investigation. The results propose a possible part for N. sativa in promoting pancreatic regeneration and warrant further exploration for its application in diabetes management.

Innovative antibacterial and antifungal coating films of chitosan/nano zinc oxide/essential oil on grape preservation

Al-Meshal,

Baakdah,

Helal

et al. 2024

The effects of some innovative antibacterial and antifungal coating films of chitosan/nano zinc oxide/essential oil (C-ZNO-EO) on grape preservation for human health were studied during the storage periods on (0, 4, 7, 14, and 16) days. Several evaluation approaches were evaluated such as the bacterial evaluations against the presence of the mesophilic aerobic bacteria. The polyamines evaluation was studied by thin-layer chromatography (TLC) for putrescine and spermidine. Soluble sugars were detected by high-performance liquid chromatography (HPLC) for fructose, glucose, and sucrose. Iron, zinc, and manganese were absorbed by the spectrum spectrophotometer. The antifungal properties have been examined against Botryis cineraa, Aspergillus niger, and Tubingensis. The results reported that the highest mesophilic aerobic bacteria (4.73 Log CFU g−1) was recorded in the control grapes on the 16th day, whereas the lowest level (2.05 Log CFU g−1) was recorded for C-ZNO-EO. The polyamines ranged from 12.18 nmol g−1 FW to 10.1 nmol g−1 FW, and from 21.35 nmol g−1 FW to 16 nmol g−1 FW for putrescine and spermidine during the storage periods, respectively. The grapes treated with C-ZNO-EO achieved the maximum increase in glucose and fructose contents (40.61 and 30.05 μmol/g FW), while grapes treated with chitosan/nano zinc oxide (C-ZNO) achieved the maximum increase in sucrose (0.982 μmol/g FW) compared with chitosan (C) and control treatments at the end of the storage periods, respectively. Using C-NZO-EO treatment had a positive effect on the mineral compositions and the antifungal against Botryis cineraa, Aspergillus niger, and Tubingensis. The innovative antibacterial and antifungal coating film by C-NZO-EO was the best treatment for grape preservation.