The Immunological Regulation Roles of Porcine β-1, 4 Galactosyltransferase V (B4GALT5) in PRRSV Infection

Abstract

B4GALT5, also known as β-1, 4 galactosyltransferase V, is one of the members of β-1, 4 galactosyltransferase gene (B4GALT) family, which was concerned with embryonic development, tumor generation, other malignant diseases. In this study, we firstly cloned porcine B4GALT (pB4GALT5) from porcine alveolar macrophages, and predicted the structural domain and function of seven porcine β-1, 4 galactosyltransferase (I–VII) based on transcriptome analysis of PRRSV infected cells. Additionally, the upregulated porcine B4GALT5 expression was detected from PRRSV infected porcine alveolar macrophage (PAM) cells. The PRRSV proliferation were slightly inhibited in overexpression of pB4GALT5 transfected cells, the interaction of B4GALT5 and GP5 of PRRSV was firstly be detected by Co-IP, and the co-location between B4GALT5 and GP5 were also observed in golgi membranes by confocal microscopy. A significant increasing mRNA transcription, including inflammatory cytokines (IFN-α, IL-6, IL-18, IL-1β, TNF-α) and some cell surface glycosylated protein involved in antigen present (MHC-I/II), cell adhesion and migration (chemokine MCP-1 and receptor CCR2; LFA-1, ICAM-1) were upregulated in B4GALT5 overexpressed PRRSV infected cells. Our results demonstrated that the regulation of pB4GALT5 plays an important roles in PRRSV proliferation and modification function in viral infection cells. And these results will make achievements by supporting the research of latent mechanisms of β-1, 4 galactosyltransferase V in antiviral immunity.

Differentially Expressed MiRNAs and tRNA Genes Affect Host Homeostasis During Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infections in Young Pigs

“…Additionally, at 3 dpi is the predicted interaction of the trna202_LeuTAG with the Beta 4-glycosyltransferase gene family that also contains genes involved in the functioning of chondroitin, a major proteoglycan pathway shown to be highly targeted by the miRNAs at each dpi. This can be seen in the results at 3 dpi where miRNAs ssc-miR-27b-3p and ssc-miR-23a-3p targeting B4GALT5 (Zhang et al, 2018) are being downregulated in the mucin type-o pathway in a possible attempt to bolster mucosal immunity in the host airway.…”

An integrative network analysis framework for identifying molecular functions in complex disorders examining major depressive disorder as a test case

“…Significant gap in enrichment analysis databases were identified for a number of glycogenes in the blood sample DEGs. These glycogenes, which are reported to be critical regulators of inflammatory responses, involve OGA ( O -GlcNAcase) and B4GALT5 (β-1,4-galactosyltransferase-5) 95 – 99 . Other few important glycogenes identified in this category are lectins involved in the plasma protein clearance, immune cell adhesion and anti-coagulant properties such as C-type lectin receptors ASGR1 (asialoglycoprotein receptor-1), DGCR2 (DiGeorge syndrome critical region gene-2) and thrombomodulin (THBD) 100 , 101 .…”

CD163 Antibodies Inhibit PRRSV Infection via Receptor Blocking and Transcription Suppression

“…Specificity was confirmed by sequencing of the PCR products and melting-curve analysis for qPCR. Relative mRNA expressions of N, CD163, NF-KB, IL-1β, and IL-8 were detected using qRT-PCR and calculated using the 2-ΔΔ Ct method [ 36 ]. Three replicates were included for each treatment.…”