The Immunomodulatory Effect of Adipose-Derived Stem Cells in Xenograft Transplantation Model

Jeon, Sungmi; Ha, Jaehyun; Kim, Iljin; Bae, Jiyoon; Kim, Sang Wha

doi:10.1016/j.transproceed.2022.06.007

Cited by 6 publications

(5 citation statements)

References 22 publications

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“…UC-MSCs + hydrogel have significant advantages over existing non-hydrogel encapsulated stem cell approaches in diabetes treatment. The above experimental results show that UC-MSCs + hydrogel can reduce oxidative stress and inflammation, promote MIN-6 cell proliferation, and restore the biological functions of MIN-6 [10][11][12]. In addition, current research in the field of non-hydrogelencapsulated stem cells for the treatment of diabetes shows that direct injection of stem cells can reduce inflammation, modulate immune responses and promote cell function.…”

Section: Uc-mscs + Hydrogel Enhanced Long-term Survival Of Injured Mi...mentioning

confidence: 80%

“…Existing methods often involve direct injection of stem cells for the treatment of diabetes. However, this approach may not provide the same protection and support for the cells as embedding in hydrogel, nor the same sustained therapeutic effect as hydrogel co-culture [11,12].…”

Section: Uc-mscs + Hydrogel Enhanced Long-term Survival Of Injured Mi...mentioning

confidence: 99%

“…Therefore, UC-MSCs are widely used in diabetes treatment. Previous studies have shown that UC-MSCs can stimulate the proliferation and regeneration of islet β-cells, improve insulin secretion and control blood glucose levels in diabetes [10,11]. UC-MSCs therapy can promote the 2 regeneration and functional recovery of β-cells, attenuate the dedifferentiation of β-cells caused by the inflammatory microenvironment in diabetes, and improve the ability to secrete insulin [12].…”

Section: Introductionmentioning

confidence: 99%

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Repair Effect of UC‐MSCs Embedded in Hydrogel on MIN‐6 Cells Injured by STZ

Yang,

Liu,

Wang

et al. 2024

Preprint

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To investigate the repair mechanism of umbilical cord mesenchymal stem cells (UC-MSCs) in hydrogel on pancreatic β-cells in diabetes, MIN-6 damaged by streptozotocin (STZ) in vitro was used in co-culture with UC-MSCs in hydrogel (UC-MSCs + hydrogel). It was found that UC-MSCs + hydrogel had a significant repair effect on injured MIN-6 cells, which was better than the use of UC-MSCs alone (without hydrogel). After repair, the expression of superoxide dismutase (SOD) and catalase (CAT) as well as the total antioxidant capacity (T-AOC) of the repaired MIN-6 cells were increased, effectively reducing the oxidative stress caused by STZ. In addition, UC-MSCs + hydrogel were able to curb the inflammatory response by promoting the expression of anti-inflammatory factor IL-10 and reducing inflammatory factor IL-1β. In addition, the expression of both nuclear antigen Ki67 for cell proliferation and insulin-related genes such as Pdx1 and MafA was increased in the repaired MIN-6 cells by UC-MSCs + hydrogel, suggesting that the repair effect promotes the proliferation of the injured MIN-6 cells. Compared with the use of UC-MSCs alone, UC-MSCs + hydrogel exhibited longer survival time of UC-MSCs, because the porous structure and hydrophilic properties of the hydrogel could affect the growth of cells and slow down their metabolic activities, resulting in a better repair effect on the injured MIN-6 cells.

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Section: Uc-mscs + Hydrogel Enhanced Long-term Survival Of Injured Mi...mentioning

confidence: 80%

Section: Uc-mscs + Hydrogel Enhanced Long-term Survival Of Injured Mi...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Repair Effect of UC‐MSCs Embedded in Hydrogel on MIN‐6 Cells Injured by STZ

Yang,

Liu,

Wang

et al. 2024

Preprint

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“…The preliminary transplantation of hair follicle-specific dermal cells allows for the reconstruction of dissected hair follicles that are grafted afterward [ 30 ]. Injecting adipose-derived stem cells into full-thickness grafts decreased the inflammatory response, promoted angiogenesis, and prevented scarring after transplantation [ 37 , 61 ]. Dermal progenitor cell transplantation beneath a split-thickness graft restores the dermis structures and improves the mechanical properties of the skin [ 43 ].…”

Section: Basic Principles In Xenotransplantationmentioning

confidence: 99%

Blank Spots in the Map of Human Skin: The Challenge for Xenotransplantation

Cherkashina,

Morgun,

Rippa

et al. 2023

IJMS

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Most of the knowledge about human skin homeostasis, development, wound healing, and diseases has been accumulated from human skin biopsy analysis by transferring from animal models and using different culture systems. Human-to-mouse xenografting is one of the fundamental approaches that allows the skin to be studied in vivo and evaluate the ongoing physiological processes in real time. Humanized animals permit the actual techniques for tracing cell fate, clonal analysis, genetic modifications, and drug discovery that could never be employed in humans. This review recapitulates the novel facts about mouse skin self-renewing, regeneration, and pathology, raises issues regarding the gaps in our understanding of the same options in human skin, and postulates the challenges for human skin xenografting.

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“…hMSCs are multipotent stem/progenitor cells found in several tissues, including bone marrow, adipose tissue, umbilical cord, placenta, and chorion [4][5][6][7]. hMSCs have been recognized as prospective cell sources for several clinical applications due to their ability to release many beneficial bioactive compounds that reduce inflammation, increase cell viability, and enhance neovascularization [8][9][10]. Additionally, some sources of hMSC, such as bone marrow and adipose tissues, can be harvested from patients and used for autologous transplantation, thus circumventing the need for immunosuppression [10,11].…”

Section: Introductionmentioning

confidence: 99%

Human Mesenchymal Stem Cells Derived from the Placenta and Chorion Suppress the Proliferation while Enhancing the Migration of Human Breast Cancer Cells

Sirithammajak

Manochantr

Tantrawatpan

et al. 2022

Stem Cells International

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Background. Breast cancer is the most frequently diagnosed malignancy among women, resulting from abnormal proliferation of mammary epithelial cells. The highly vascularized nature of breast tissue leads to a high incidence of breast cancer metastases, resulting in a poor survival rate. Previous studies suggest that human mesenchymal stem cells (hMSCs) play essential roles in the growth, metastasis, and drug responses of many cancers, including breast cancer. However, hMSCs from different sources may release different combinations of cytokines that affect breast cancer differently. Methods. In this study, we have isolated hMSCs from the placenta (PL-hMSCs) and the chorion (CH-hMSCs) and determined how these hMSCs affect the proliferation, migration, invasion, and gene expression of two human breast cancer cells, MCF-7 and MDA-MB-231, as well as the possible mechanisms underlying those effects. Results. The results showed that the soluble factors derived from PL-hMSCs and CH-hMSCs inhibited the proliferation of MCF-7 and MDA-MB-231 cells but increased the migration of MDA-MB-231 cells. The study of gene expression showed that PL-hMSCs and CH-hMSCs downregulated the expression levels of the protooncogene CyclinD1 while upregulating the expression levels of tumor suppressor genes, P16 and P21 in MCF-7 and MDA-MB-231 cells. Furthermore, hMSCs from both sources also increased the expression levels of MYC, SNAI1, and TWIST, which promote the epithelial-mesenchymal transition and migration of breast cancer cells in both cell lines. The functional study suggests that the suppressive effect of CH-hMSCs and PL-hMSCs on MCF-7 and MDA-MB231 cell proliferation was mediated, at least in part, through IFN-γ. Conclusions. Our study suggests that CH-hMSCs and PL-hMSCs inhibited breast cancer cell proliferation by negatively regulating CYCLIND1 expression and upregulating the expression of the P16 and P21 genes. In contrast, hMSCs from both sources enhanced breast cancer cell migration, possibly by increasing the expression of MYC, SNAI1, and TWIST genes in those cells.

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The Immunomodulatory Effect of Adipose-Derived Stem Cells in Xenograft Transplantation Model

Cited by 6 publications

References 22 publications

Repair Effect of UC‐MSCs Embedded in Hydrogel on MIN‐6 Cells Injured by STZ

Repair Effect of UC‐MSCs Embedded in Hydrogel on MIN‐6 Cells Injured by STZ

Blank Spots in the Map of Human Skin: The Challenge for Xenotransplantation

Human Mesenchymal Stem Cells Derived from the Placenta and Chorion Suppress the Proliferation while Enhancing the Migration of Human Breast Cancer Cells

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