The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy

Navrátilová, Iva; Skládal, Petr

doi:10.1016/j.bioelechem.2003.10.004

Cited by 100 publications

(51 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[306][307][308][309][310][311][312][313] Another option is based on an amine-terminated self-assembled monolayer, which reacts with glutaraldehyde. 314,315 Biomolecules can then be immobilized on the surface of the electrode through the reaction of terminal -NH 2 group. A vast number of strategies, protocols and methods exist for the controlled immobilization of biomolecules onto surfaces.…”

Section: Accumulation Sensorsmentioning

confidence: 99%

Electrode Materials (Bulk Materials and Modification)

Walcarius

Etienne

Herzog

et al. 2014

Environmental Analysis by Electrochemical Sensors and Biosensors

View full text Add to dashboard Cite

Section: Accumulation Sensorsmentioning

confidence: 99%

Electrode Materials (Bulk Materials and Modification)

Walcarius

Etienne

Herzog

et al. 2014

Environmental Analysis by Electrochemical Sensors and Biosensors

View full text Add to dashboard Cite

“…For practical use, direct monitoring of impedance value at a fixed frequency seems to be satisfactory and so far is preferable [12]. The impedance data obtained for each sensor were analyzed at a frequency 1 Hz (Fig.…”

Section: Calibration Curvesmentioning

confidence: 99%

“…As a biosensitive element it is theoretically possible to use antibodies (Abs), aptamers, receptors, enzymes etc. And if high-affinity aptamers [10], membrane-anchored receptors [11] or monoclonal Abs [12] against small molecules of interest have to be obtained using rather complicated procedures, some mammalian enzymes proven to be natural analyte targets are commercially available.…”

Section: Introductionmentioning

confidence: 99%

Nonfaradaic Impedance Probing of Potato Glycoalkaloids Interaction with Butyrylcholinesterase Immobilized onto Gold Electrode

2006

View full text Add to dashboard Cite

In this paper, we report the development of enzyme-based nonfaradaic impedimetric biosensor for direct detection of potato glycoalkaloids a-chaconine and a-solanine. The sensors were prepared by cross-linking of horse butyrylcholinesterase (BuChE) onto gold electrodes. Affinity interaction of both glycoalkaloids with immobilized BuChE caused an essential decrease of the total impedance and of the interfacial polarization resistance as well. No effect was observed after a-chaconine contact with bovine serum albumin and bacterial carboxylesterase immobilized onto gold. BuChE-modified electrodes were more sensitive to a-chaconine, and found I 50 values were in quite good agreement with those measured by biocatalytic ISFET-based sensors.

show abstract

“…Electrochemical Impedance Spectroscopy (EIS) is an effective tool for probing the feature surface-modified electrode while controlling its electrical properties [33,34]. In a first time, this technique was used for the characterization of the impedance behavior of SAM modified gold electrode and for subsequent extraction of useful information from this.…”

Section: Impedance Measurementmentioning

confidence: 99%

Sensitivity Improvement of an Impedimetric Immunosensor Using Functionalized Iron Oxide Nanoparticles

et al. 2009

View full text Add to dashboard Cite

This work has explored the development of impedimetric immunosensors based on magnetic iron nanoparticles (IrNP) functionalized with streptavidin to which a biotinylated FAB part of the antibody has been bound using a biotin-streptavidin interaction. SPR analysis shows a deviation on the measured (angle) during antigen-antibody recognition whereas label free detection using by EIS allows us to monitor variation of polarization resistance. Before detection, layers were analyzed by FTIR and AFM. Compared to immobilization of antibody on bare gold surface using aminodecanethiol SAM, antibody immobilization on nanoparticles permitted to reach lower detection limit: 500 pg/ml instead of 1 ng/ml to in the case of EIS and 300 ng/ml instead of 4.5 μg/ml in the case of SPR. Thus, it permitted to improve the sensitivity: from 257.3 Ω · cm 2 · μg −1 · ml to 1871 Ω · cm 2 · μg −1 · ml in the case of EIS and from 0.003• μg −1 · ml to 0.094 • μg −1 · ml in the case of SPR.

show abstract

The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy

Cited by 100 publications

References 23 publications

Electrode Materials (Bulk Materials and Modification)

Electrode Materials (Bulk Materials and Modification)

Nonfaradaic Impedance Probing of Potato Glycoalkaloids Interaction with Butyrylcholinesterase Immobilized onto Gold Electrode

Sensitivity Improvement of an Impedimetric Immunosensor Using Functionalized Iron Oxide Nanoparticles

Contact Info

Product

Resources

About