The immunostimulatory effects and chemical characteristics of heated honey

Ota, Misato; Ishiuchi, Kan’ichiro; Xu, Xin; Minami, Masaaki; Nagachi, Yasutaka; Yagi-Utsumi, Maho; Tabuchi, Yoshiaki; Cai, Shao‐Qing; Makino, Toshiaki

doi:10.1016/j.jep.2018.09.019

Cited by 13 publications

(13 citation statements)

References 30 publications

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“…We found the amount of turanose in honey was significantly correlated with the activity of honey after heating at 180 °C for 30 min. However, turanose did not exhibit the activity in this heating condition but was heated for more than 2 h. For isomaltose, the activity appeared after it was heated to 180 °C for 20 min, and the time-and temperature-dependent manners of isomaltose exhibited were similar to those of honey in our previous study 4 .…”

Section: Discussionsupporting

confidence: 67%

“…We screened the suitable temperature and heating duration required for isomaltose to induce G-CSF secretion, and the greatest effect appeared when isomaltose was heated at 150 °C for 240 min, at 180 °C for 60 min, or at 200 °C for 15-30 min, which was similar to the results of honey and licorice roasted with honey from our previous study 4,13 . We surmise that certain novel active compounds were produced when honey was heated but degraded upon excess heating.…”

Section: Discussionmentioning

confidence: 53%

“…The measurement of the sugar contents was conducted by an external standard method, and the quantitative ranges for each sugar were as 17.5-70.0 µg/ml for fructose and glucose, and 0.11-3.57 µg/ml for the other sugars. Size-exclusion HPLC was performed according to the method described in our previous study 4 .…”

Section: Analysis Of the Sugar Contents And Molecular Weights By Highmentioning

confidence: 99%

“…In order to assess immunostimulatory activity, experiments were conducted similarly to our previous investigation 4 . Briefly, murine colonic epithelial MCE301 cells 46 were cultured in Dulbecco's modified Eagle medium/Ham's F-12 (1:1) (DMEM/F-12) medium (Sigma Aldrich, St. Louis, MO, USA) containing 5% fetal bovine serum (FBS; Sigma), 100 units/ml penicillin and 100 µg/ml streptomycin (both from Nacalai).…”

Section: Assessment Of Immunostimulatory Activity and The Signaling Pmentioning

confidence: 99%

“…Previously, we discovered that heat-processed honey but not unheated honey could induce the secretion of granulocyte-colony stimulating factor (G-CSF) in the MCE301 intestinal epithelial cells 4 . G-CSF is a cytokine that promotes the proliferation, differentiation, and maturation of granulocytes 5 , 6 and regulates hematopoiesis and innate/adaptive immunity 7 , 8 .…”

Section: Introductionmentioning

confidence: 99%

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Honey isomaltose contributes to the induction of granulocyte-colony stimulating factor (G-CSF) secretion in the intestinal epithelial cells following honey heating

Asai

Kato

et al. 2020

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We have previously discovered that heated honey but not unheated honey could induce the secretion of granulocyte-colony stimulating factor (G-CSF) in the MCE301 intestinal epithelial cells. The objective of this study was to identify compounds in honey that could contribute to this activity. We bought several kinds of commercial honey samples derived from different flowers, as well as corn syrup samples, in the markets of China and Japan, and heated them at 180 °C for 30 min. MCE301 cells were treated with the medium containing the samples, and G-CSF levels in the medium were measured by ELISA. By comparing their activities and sugar contents, we discovered that isomaltose was primarily implicated. The optimum heating conditions for isomaltose were at 180 °C for 60 min or at 200 °C for 15–30 min, and these time- and temperature-dependencies were similar to those of honey in our previous study. When heated isomaltose was partitioned by dialysis, the active ingredients were transferred into a high-molecular-weight fraction. By size-exclusion HPLC analysis, the average molecular weight of heated isomaltose was 790 kDa. When heated isomaltose was hydrolyzed by acids, glucose was subsequently produced. Maltose, sucrose, turanose, and trehalose did not exhibited any activity when heated at 180 °C for 60 min, indicating that the glucose groups with α(1 → 6)-binding in the isomaltose molecule play important roles in its activity when oxidatively polymerized by heat. The stimulating activity of heated isomaltose was inhibited by toll-like receptor 4 (TLR4) inhibitor, suggesting that heated isomaltose activates TLR4 to induce G-CSF. Since G-CSF is clinically used for cancer patients to accelerate their recovery from neutropenia following chemotherapy or accompanied with aplastic anemia, these findings indicate that honey which contains high level of isomaltose could improve immunosuppressive conditions when honey is heated, and that heated isomaltose might be of potential therapeutic use in patients with compromised immunity caused by chemotherapeutic agents.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 53%

Section: Analysis Of the Sugar Contents And Molecular Weights By Highmentioning

confidence: 99%

Section: Assessment Of Immunostimulatory Activity and The Signaling Pmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Honey isomaltose contributes to the induction of granulocyte-colony stimulating factor (G-CSF) secretion in the intestinal epithelial cells following honey heating

Asai

Kato

et al. 2020

Sci Rep

Self Cite

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Evaluation of physical, biochemical properties and cell viability of gamma irradiated honey

et al. 2021

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Antimicrobial activity of commercial organic honeys against clinical isolates of human pathogenic bacteria

et al. 2022

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Extracts of five organic and one conventional honey sources, available in Finnish markets, were tested for antimicrobial activity and inhibitory concentrations against Escherichia coli, Salmonella Typhi, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus cereus, and Staphylococcus epidermidis, obtained from human specimens. Five (honeys A, B, D, E, F) of the six studied honeys were organic. All the studied honeys had inhibitory activity (zone of inhibition (ZI) > 9.4 ± 0.5 mm) compared to control artificial honey (ZI < 8 mm) against all the studied pathogens. Four organic honeys (B, D, E, F) showed inhibitory activity with ZI > 13.3 mm against all the studied bacteria with minimal inhibitory concentration (MIC) values of 12.5–50%. Against E. coli, the organic honeys E and F had activity index (AI) of 0.64 and 0.73, respectively, compared to the antibiotic AI of 1.0. Against S. Typhi, the organic honeys D and F had AI of 0.59 and 0.64, respectively. Against P. aeruginosa, the organic honeys D, E, and F had the highest AI of 0.71–0.80, and against S. epidermidis the honeys B, D, E, and F possessed relatively high AI of 0.60, 0.67, 0.73, and 0.78, respectively. Against K. pneumoniae and B. cereus, the detected AI of the organic honeys B, D, E, and F varied between AI of 0.48 and 0.58. The organic honey A and conventionally produced honey C possessed only minor activity with MIC values of 80%. Here, we show that commercially available culinary organic honeys possess remarkable antimicrobial activity against several important human bacterial pathogens.

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The immunostimulatory effects and chemical characteristics of heated honey

Cited by 13 publications

References 30 publications

Honey isomaltose contributes to the induction of granulocyte-colony stimulating factor (G-CSF) secretion in the intestinal epithelial cells following honey heating

Honey isomaltose contributes to the induction of granulocyte-colony stimulating factor (G-CSF) secretion in the intestinal epithelial cells following honey heating

Evaluation of physical, biochemical properties and cell viability of gamma irradiated honey

Antimicrobial activity of commercial organic honeys against clinical isolates of human pathogenic bacteria

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