The impact of ABCB1 gene polymorphism and its expression on non-small-cell lung cancer development, progression and therapy – preliminary report

Abstract

the ABCB1 gene belongs to Atp binding cassette (ABc) transporter genes that has been previously implicated in cancer progression and drug response. this study aimed to evaluate the association between the SNP 3435 and the expression of the ABCB1 gene in lung cancer patients in the polish population in comparison to clinicopathological parameters and treatment. 150 RNA and 47 DNA samples were isolated from 49 lung cancer cases including both tissue samples and blood taken from the same patients at three time points: diagnosis, 100 days and one year after the surgical intervention. Qualitative and real-time pcR analysis of expression were done, also genotyping by PCR-RFLP. Mutant homozygous TT and allele T are present statistically significantly more frequently in the group of patients with lung cancer. There is no difference with expression level in lung cancer tissue and blood sample taken from the same patients before surgical treatment. on the basis of blood samples analysis it was observed that the expression level of ABCB1 mRnA was growing in time. Higher levels were marked after 100 days and one year after the surgical intervention. The complementary pharmacological treatment induced higher expression levels of ABCB1. the presented data suggest an important role of ABCB1 in lung cancer, the increasing level of ABCB1 mRnA which can be connected with induction of multidrug resistance mechanism is also significant, that observation must be confirmed in further analysis.

Advanced bioinformatic analysis and pathway prediction of NSCLC cells upon cisplatin resistance

“…Wnt/β-catenin pathway is an important regulator of many cellular processes, including cell proliferation, development, tumorigenesis, and homeostasis 53 , 54 . This analysis correlates with several prior studies, which have associated the Wnt/β-catenin pathway activation to chemoresistance through the upregulation of the ABCB1 gene in several cancer types 55 , 56 , including LC 57 , 58 . Our RNA-seq data present differentially increased Wnt genes, including WNT5A, WNT7B, and a 120-fold increase of the ABCB1 gene.…”

The role of ABCB1 gene polymorphisms in steroid‐resistant nephrotic syndrome: Evidence from a meta‐analysis of steroid‐receiving patients

“…The ABCB1 gene is highly polymorphic in nature, and it has been reported that multiple polymorphisms of this gene may be associated with P-gp, which is linked with various processes such as apoptosis, cellular proliferation, differentiation, and immune system mechanisms. 38,39 Genetic polymorphisms are correlated with the alterations in the quantity or activity of different membrane transporters or proteins, leading to a change in the physiological activities, which further results in disease progression. 40,41 As a result of the characteristic polymorphisms, the ABCB1 gene has been widely investigated, and approximately 66 SNPs of this gene have been identified, among which 22 SNPs are synonymous and others are non-synonymous.…”

Increased expressions of cellular ATP-binding cassette transporters may be a promising diagnostic marker for colorectal cancer

“…However, the ABCB1 mRNA level grew over time in blood samples taken from lung cancer patients, suggesting an important contribution of ABCB1 in lung cancer MDR. 37 In the ABCC1 and ABCG2 genes, the alteration in the expression and the effectiveness of the corresponding gene and its product were located in the single nucleotide polymorphisms (SNPs). Thus, they might elevate the risk and impact the response of the cancer cells to the treatment and increase the capability of other types of cancer.…”